##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AflII AgeI Alw44I
AlwI Aor51HI ApaLI AscI Asp718I
AspI BalI BamHI BciVI BfrI
BglI BglII BsiWI Bsp1407I BsrDI
BsrGI BssSI BstBI BstYI CpoI
DraIII DrdI Eco47III EcoRI EcoT22I
FseI HindIII HpaI KpnI MfeI
MflI MluI MluNI MscI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PflFI PinAI
PmeI PvuI RsrII SalI SapI
SfiI SfuI SgrAI SnaBI SphI
SspBI SwaI Tth111I XbaI XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccIII 4205 TCCGGA T^CCGGA
AclI 2284 AACGTT AA^CGTT
AcsI 2455 AAATTT [GA]^AATT[CT]
ApoI 2455 AAATTT [GA]^AATT[CT]
AseI 4057 ATTAAT AT^TAAT
AsnI 4057 ATTAAT AT^TAAT
Asp700I 3942 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1202 TGCGCA TGC^GCA
AvrII 1879 CCTAGG C^CTAGG
BbeI 3086 GGCGCC GGCGC^C
BbrPI 4196 CACGTG CAC^GTG
BcgI 2862 GCACCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1879 CCTAGG C^CTAGG
BsaAI 4196 CACGTG [CT]AC^GT[GA]
BsaBI 1740 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4205 TCCGGA T^CCGGA
BsiEI 3020 CGGCCG CG[GA][CT]^CG
BsmBI 3359 CGTCTC CGTCTC(1/5)
BspDI 2144 ATCGAT AT^CGAT
BspEI 4205 TCCGGA T^CCGGA
BsrBI 2523 GAGCGG CCGCTC(-3/-3)
BsrFI 2929 GCCGGT [GA]^CCGG[CT]
BssHII 2904 GCGCGC G^CGCGC
Bst1107I 1360 GTATAC GTA^TAC
BstAPI 4000 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 295 GGTGACC G^GT[ACGT]ACC
BstPI 295 GGTGACC G^GT[ACGT]ACC
BstZ17I 1360 GTATAC GTA^TAC
Cfr10I 2929 GCCGGT [GA]^CCGG[CT]
ClaI 2144 ATCGAT AT^CGAT
DraI 2138 TTTAAA TTT^AAA
EaeI 3020 CGGCCG [CT]^GGCC[GA]
EagI 3020 CGGCCG C^GGCCG
EclXI 3020 CGGCCG C^GGCCG
Eco52I 3020 CGGCCG C^GGCCG
EcoO65I 295 GGTGACC G^GT[ACGT]ACC
EcoRV 3014 GATATC GAT^ATC
FspI 1202 TGCGCA TGC^GCA
HincII 3300 GTTGAC GT[CT]^[GA]AC
HindII 3300 GTTGAC GT[CT]^[GA]AC
KasI 3086 GGCGCC G^GCGCC
MamI 1740 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4205 TCCGGA T^CCGGA
NarI 3086 GGCGCC GG^CGCC
PacI 163 TTAATTAA TTAAT^TAA
PflMI 286 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4196 CACGTG CAC^GTG
PmlI 4196 CACGTG CAC^GTG
PshAI 2912 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4057 ATTAAT AT^TAAT
Psp1406I 2284 AACGTT AA^CGTT
SbfI 3094 CCTGCAGG CCTGCA^GG
SexAI 2546 ACCTGGT A^CC[AT]GGT
SfoI 3086 GGCGCC GGC^GCC
SpeI 2176 ACTAGT A^CTAGT
Sse8387I 3094 CCTGCAGG CCTGCA^GG
StuI 4179 AGGCCT AGG^CCT
Van91I 286 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1125 CCAACTGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3942 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1360 GTATAC GT^[AC][GT]AC
4045 GTCTAC
AflIII 197 ACATGT A^C[GA][CT]GT
687 ACATGT
AhdI 872 GACATTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4142 GACACTGGGTC
AlwNI 287 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1811 CAGTATCTG
AocI 1014 CCTCAGG CC^T[ACGT]AGG
3849 CCTGAGG
ApaI 902 GGGCCC GGGCC^C
3048 GGGCCC
AspEI 872 GACATTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4142 GACACTGGGTC
BclI 1304 TGATCA T^GATCA
4078 TGATCA
BsaWI 2834 ACCGGA [AT]^CCGG[AT]
4205 TCCGGA
BspHI 2494 TCATGA T^CATGA
4174 TCATGA
BspLU11I 197 ACATGT A^CATGT
687 ACATGT
BspMI 4005 ACCTGC ACCTGC(4/8)
485 GCAGGT
BstXI 212 CCAGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
659 CCAGGATTGTGG
Bsu36I 1014 CCTCAGG CC^T[ACGT]AGG
3849 CCTGAGG
Eam1105I 872 GACATTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4142 GACACTGGGTC
EarI 774 GAAGAG CTCTTC(1/4)
4129 GAAGAG
Eco81I 1014 CCTCAGG CC^T[ACGT]AGG
3849 CCTGAGG
FbaI 1304 TGATCA T^GATCA
4078 TGATCA
HaeII 1448 GGCGCT [GA]GCGC^[CT]
3086 GGCGCC
Ksp632I 774 GAAGAG CTCTTC(1/4)
4129 GAAGAG
KspI 3070 CCGCGG CCGC^GG
3123 CCGCGG
NcoI 3283 CCATGG C^CATGG
4116 CCATGG
NdeI 182 CATATG CA^TATG
1405 CATATG
PvuII 3009 CAGCTG CAG^CTG
4167 CAGCTG
RcaI 2494 TCATGA T^CATGA
4174 TCATGA
SacII 3070 CCGCGG CCGC^GG
3123 CCGCGG
ScaI 2113 AGTACT AGT^ACT
2226 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI