##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AscI AseI AsnI AspEI AspI
AvrII BamHI BbrPI BbsI BglII
BlnI BpuAI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BstBI ClaI CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I HindIII
MamI MluI MroI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII ScaI
SfiI SfuI SnaBI SpeI SphI
SspBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2156 GGTACC G^GTACC
AcsI 4036 AAATTC [GA]^AATT[CT]
AflII 4046 CTTAAG C^TTAAG
AflIII 4098 ACACGT A^C[GA][CT]GT
Alw44I 3293 GTGCAC G^TGCAC
ApaLI 3293 GTGCAC G^TGCAC
ApoI 4036 AAATTC [GA]^AATT[CT]
Asp718I 2156 GGTACC G^GTACC
BbvCI 2872 CCTCAGC CCTCAGC(-5/-2)
BciVI 1848 GGATAC GTATCC(6/5)
BclI 1838 TGATCA T^GATCA
BfrI 4046 CTTAAG C^TTAAG
BsaAI 925 TACGTG [CT]AC^GT[GA]
BsaWI 4004 ACCGGA [AT]^CCGG[AT]
BsgI 643 CTGCAC GTGCAG(16/14)
BsmI 2029 GAATGC GAATGC(1/-1)
Bst1107I 4072 GTATAC GTA^TAC
BstEII 4000 GGTCACC G^GT[ACGT]ACC
BstPI 4000 GGTCACC G^GT[ACGT]ACC
BstXI 767 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4072 GTATAC GTA^TAC
DrdI 2909 GACTGCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1165 CGGCCG C^GGCCG
EclXI 1165 CGGCCG C^GGCCG
Eco52I 1165 CGGCCG C^GGCCG
EcoO65I 4000 GGTCACC G^GT[ACGT]ACC
FbaI 1838 TGATCA T^GATCA
FseI 1510 GGCCGGCC GGCCGG^CC
HpaI 2436 GTTAAC GTT^AAC
KpnI 2156 GGTACC GGTAC^C
MfeI 2259 CAATTG C^AATTG
MunI 2259 CAATTG C^AATTG
NdeI 2403 CATATG CA^TATG
NheI 3214 GCTAGC G^CTAGC
NspI 3370 ACATGC [GA]CATG^[CT]
SalI 2906 GTCGAC G^TCGAC
SapI 62 GCTCTTC GCTCTTC(1/4)
SbfI 936 CCTGCAGG CCTGCA^GG
SexAI 37 ACCAGGT A^CC[AT]GGT
SgrAI 868 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 936 CCTGCAGG CCTGCA^GG
SspI 3756 AATATT AAT^ATT
StuI 349 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 2786 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3185 CAGGAGCTG
AocI 249 CCTAAGG CC^T[ACGT]AGG
2294 CCTCAGG
Asp700I 1113 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2470 GAAGGTTTTC
BbeI 1499 GGCGCC GGCGC^C
3125 GGCGCC
BsmBI 4101 CGTCTC CGTCTC(1/5)
3047 GAGACG
BsrDI 3774 CATTGC GCAATG(2/0)
2189 GCAATG
BstYI 3000 AGATCC [GA]^GATC[CT]
3658 AGATCC
Bsu36I 249 CCTAAGG CC^T[ACGT]AGG
2294 CCTCAGG
Eco81I 249 CCTAAGG CC^T[ACGT]AGG
2294 CCTCAGG
HincII 2436 GTTAAC GT[CT]^[GA]AC
2906 GTCGAC
HindII 2436 GTTAAC GT[CT]^[GA]AC
2906 GTCGAC
KasI 1499 GGCGCC G^GCGCC
3125 GGCGCC
MflI 3000 AGATCC [GA]^GATC[CT]
3658 AGATCC
MslI 2229 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2441 CACAAGGATG
NaeI 225 GCCGGC GCC^GGC
1511 GCCGGC
NarI 1499 GGCGCC GG^CGCC
3125 GGCGCC
NcoI 614 CCATGG C^CATGG
3579 CCATGG
NgoMIV 225 GCCGGC G^CCGGC
1511 GCCGGC
PaeR7I 231 CTCGAG C^TCGAG
3525 CTCGAG
SacI 2675 GAGCTC GAGCT^C
2984 GAGCTC
SfoI 1499 GGCGCC GGC^GCC
3125 GGCGCC
XcmI 605 CCAAGGGCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2515 CCACATCTTGTGTGG
XhoI 231 CTCGAG C^TCGAG
3525 CTCGAG
XhoII 3000 AGATCC [GA]^GATC[CT]
3658 AGATCC
XmnI 1113 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2470 GAAGGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI Aor51HI ApaI AspHI
AvaI AvaII AviII BalI BanI
BanII BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmaCI
XmaI