##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BclI BfrI BglII
BlnI BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI Eam1105I EcoO65I EcoRI EcoT22I
FbaI FspI HpaI KpnI MamI
MluI MroI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1168 GACGTC GACGT^C
AccI 2574 GTCTAC GT^[AC][GT]AC
AclI 2251 AACGTT AA^CGTT
AcsI 2288 GAATTT [GA]^AATT[CT]
AflIII 1035 ACATGT A^C[GA][CT]GT
Alw44I 3923 GTGCAC G^TGCAC
AocI 3816 CCTTAGG CC^T[ACGT]AGG
Aor51HI 782 AGCGCT AGC^GCT
ApaLI 3923 GTGCAC G^TGCAC
ApoI 2288 GAATTT [GA]^AATT[CT]
Asp700I 336 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1165 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3643 GGATCC G^GATCC
BbrPI 1853 CACGTG CAC^GTG
BcgI 295 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 289 GTATCC GTATCC(6/5)
BspLU11I 1035 ACATGT A^CATGT
Bsu36I 3816 CCTTAGG CC^T[ACGT]AGG
Eco47III 782 AGCGCT AGC^GCT
Eco81I 3816 CCTTAGG CC^T[ACGT]AGG
FseI 4423 GGCCGGCC GGCCGG^CC
HincII 1504 GTCAAC GT[CT]^[GA]AC
HindII 1504 GTCAAC GT[CT]^[GA]AC
HindIII 2478 AAGCTT A^AGCTT
MfeI 2318 CAATTG C^AATTG
MunI 2318 CAATTG C^AATTG
NcoI 1460 CCATGG C^CATGG
PflFI 1165 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1853 CACGTG CAC^GTG
PmlI 1853 CACGTG CAC^GTG
Psp1406I 2251 AACGTT AA^CGTT
SexAI 4287 ACCTGGT A^CC[AT]GGT
SspI 3502 AATATT AAT^ATT
Tth111I 1165 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2698 TCTAGA T^CTAGA
XmnI 336 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 121 GTAC GT^AC
3424 GTAC
BbeI 806 GGCGCC GGCGC^C
1474 GGCGCC
BglI 447 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
505 GCCTGGCGGGC
BsrBI 607 CCGCTC CCGCTC(-3/-3)
206 GAGCGG
BssHII 481 GCGCGC G^CGCGC
4385 GCGCGC
BssSI 2072 CACGAG CACGAG(-5/-1)
950 CTCGTG
DraIII 699 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2611 CACCCAGTG
EcoNI 2764 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3731 CCTTCACTAGG
EcoRV 1444 GATATC GAT^ATC
3487 GATATC
KasI 806 GGCGCC G^GCGCC
1474 GGCGCC
KspI 245 CCGCGG CCGC^GG
568 CCGCGG
NarI 806 GGCGCC GG^CGCC
1474 GGCGCC
PaeR7I 1509 CTCGAG C^TCGAG
3684 CTCGAG
PflMI 640 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1863 CCATCAGGTGG
PshAI 959 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3573 GACCTTTGTC
RsaI 121 GTAC GT^AC
3424 GTAC
SacI 850 GAGCTC GAGCT^C
4268 GAGCTC
SacII 245 CCGCGG CCGC^GG
568 CCGCGG
SapI 276 GCTCTTC GCTCTTC(1/4)
467 GCTCTTC
SfoI 806 GGCGCC GGC^GCC
1474 GGCGCC
SmaI 3896 CCCGGG CCC^GGG
4482 CCCGGG
StuI 2679 AGGCCT AGG^CCT
3882 AGGCCT
Van91I 640 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1863 CCATCAGGTGG
XhoI 1509 CTCGAG C^TCGAG
3684 CTCGAG
XmaCI 3896 CCCGGG C^CCGGG
4482 CCCGGG
XmaI 3896 CCCGGG C^CCGGG
4482 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII