##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI BbeI BfrI BglII
BsaBI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BsrBI BsrFI
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FseI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfoI SfuI SgrAI SnaBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2029 GACGTC GACGT^C
AcsI 433 GAATTT [GA]^AATT[CT]
Aor51HI 518 AGCGCT AGC^GCT
ApoI 433 GAATTT [GA]^AATT[CT]
AspI 812 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4087 TGCGCA TGC^GCA
BamHI 2324 GGATCC G^GATCC
BciVI 756 GTATCC GTATCC(6/5)
BclI 945 TGATCA T^GATCA
BsaWI 3019 ACCGGA [AT]^CCGG[AT]
BsmI 2848 GCATTC GAATGC(1/-1)
BssSI 643 CACGAG CACGAG(-5/-1)
Eco47III 518 AGCGCT AGC^GCT
EcoT22I 3527 ATGCAT ATGCA^T
FbaI 945 TGATCA T^GATCA
FspI 4087 TGCGCA TGC^GCA
HincII 2184 GTTGAC GT[CT]^[GA]AC
HindII 2184 GTTGAC GT[CT]^[GA]AC
NdeI 2770 CATATG CA^TATG
NsiI 3527 ATGCAT ATGCA^T
PflFI 812 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 1169 GAAGAGC GCTCTTC(1/4)
SbfI 590 CCTGCAGG CCTGCA^GG
SmlI 3718 CTTGAG C^T[CT][GA]AG
SpeI 1151 ACTAGT A^CTAGT
SphI 3140 GCATGC GCATG^C
Sse8387I 590 CCTGCAGG CCTGCA^GG
Tth111I 812 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1580 GGCGTC G[GA]^CG[CT]C
2029 GACGTC
Alw44I 291 GTGCAC G^TGCAC
867 GTGCAC
ApaLI 291 GTGCAC G^TGCAC
867 GTGCAC
Asp700I 835 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1558 GAAGTTGTTC
AvrII 1055 CCTAGG C^CTAGG
2952 CCTAGG
BcgI 3954 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
915 GCAAGAGTGTCG
BlnI 1055 CCTAGG C^CTAGG
2952 CCTAGG
BsaHI 1580 GGCGTC G[GA]^CG[CT]C
2029 GACGTC
BsaI 8 GGTCTC GGTCTC(1/5)
46 GGTCTC
Bsp1407I 984 TGTACA T^GTACA
2057 TGTACA
BspLU11I 2054 ACATGT A^CATGT
4264 ACATGT
BsrDI 1430 GCAATG GCAATG(2/0)
2996 GCAATG
BsrGI 984 TGTACA T^GTACA
2057 TGTACA
BssHII 2567 GCGCGC G^CGCGC
2569 GCGCGC
Hin1I 1580 GGCGTC G[GA]^CG[CT]C
2029 GACGTC
PflMI 204 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3635 CCAGGCTCTGG
PshAI 177 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3912 GACCCAGGTC
SfiI 958 GGCCTTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2166 GGCCCAGCTGGCC
SmaI 1104 CCCGGG CCC^GGG
1514 CCCGGG
SspBI 984 TGTACA T^GTACA
2057 TGTACA
TaqI 509 TCGA T^CGA
1618 TCGA
TthHB8I 509 TCGA T^CGA
1618 TCGA
Van91I 204 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3635 CCAGGCTCTGG
XcmI 1203 CCATCTGTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3631 CCACCCAGGCTCTGG
XmaCI 1104 CCCGGG C^CCGGG
1514 CCCGGG
XmaI 1104 CCCGGG C^CCGGG
1514 CCCGGG
XmnI 835 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1558 GAAGTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XhoII