##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AflII AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BbsI BcgI BclI
BfrI BpuAI BsaAI BsiEI BsiWI
BspDI BsrBI BssHII BssSI BstBI
BstEII BstPI ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FbaI FseI FspI
HaeII HpaI KasI KspI MluI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3985 GGTACC G^GTACC
AccII 1617 CGCG CG^CG
AccIII 1987 TCCGGA T^CCGGA
AcyI 1971 GGCGTC G[GA]^CG[CT]C
AgeI 1445 ACCGGT A^CCGGT
Asp718I 3985 GGTACC G^GTACC
AspI 4048 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2009 CCTCAGC CCTCAGC(-5/-2)
BciVI 541 GTATCC GTATCC(6/5)
BglI 2395 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3454 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1971 GGCGTC G[GA]^CG[CT]C
BseAI 1987 TCCGGA T^CCGGA
BsmBI 2036 CGTCTC CGTCTC(1/5)
BspEI 1987 TCCGGA T^CCGGA
BsrDI 3426 GCAATG GCAATG(2/0)
BsrFI 1445 ACCGGT [GA]^CCGG[CT]
Bst1107I 613 GTATAC GTA^TAC
BstAPI 1672 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1617 CGCG CG^CG
BstZ17I 613 GTATAC GTA^TAC
Cfr10I 1445 ACCGGT [GA]^CCGG[CT]
DraI 735 TTTAAA TTT^AAA
DraIII 1040 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 1971 GGCGTC G[GA]^CG[CT]C
HincII 1974 GTCAAC GT[CT]^[GA]AC
HindII 1974 GTCAAC GT[CT]^[GA]AC
HindIII 944 AAGCTT A^AGCTT
KpnI 3985 GGTACC GGTAC^C
MamI 3454 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2468 CAATTG C^AATTG
MroI 1987 TCCGGA T^CCGGA
MunI 2468 CAATTG C^AATTG
MvnI 1617 CGCG CG^CG
PaeR7I 1713 CTCGAG C^TCGAG
PflFI 4048 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1445 ACCGGT A^CCGGT
PshAI 1282 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 2325 AGTACT AGT^ACT
SmaI 1369 CCCGGG CCC^GGG
SphI 3111 GCATGC GCATG^C
TaqI 1714 TCGA T^CGA
Tth111I 4048 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1714 TCGA T^CGA
XhoI 1713 CTCGAG C^TCGAG
XmaCI 1369 CCCGGG C^CCGGG
XmaI 1369 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 2530 GTGCAC G^TGCAC
2868 GTGCAC
ApaLI 2530 GTGCAC G^TGCAC
2868 GTGCAC
Asp700I 2942 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2960 GAAGGGCTTC
BamHI 1990 GGATCC G^GATCC
3001 GGATCC
BanI 3936 GGCACC G^G[CT][GA]CC
3985 GGTACC
BlpI 923 GCTCAGC GC^T[ACGT]AGC
1662 GCTCAGC
Bpu1102I 923 GCTCAGC GC^T[ACGT]AGC
1662 GCTCAGC
BsaI 2551 GGTCTC GGTCTC(1/5)
3132 GGTCTC
BsmI 2177 GAATGC GAATGC(1/-1)
3777 GCATTC
BstXI 319 CCATGAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2662 CCAAGTAAGTGG
CelII 923 GCTCAGC GC^T[ACGT]AGC
1662 GCTCAGC
CfoI 1302 GCGC GCG^C
1578 GCGC
EcoNI 3666 CCTGAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3700 CCTTGCAGAGG
EcoRV 397 GATATC GAT^ATC
3454 GATATC
FauI 1747 CCCGC CCCGC(4/6)
1684 GCGGG
HhaI 1302 GCGC GCG^C
1578 GCGC
HinP1I 1302 GCGC G^CGC
1578 GCGC
MaeII 2035 ACGT A^CGT
3759 ACGT
MspA1I 2305 CAGCTG C[AC]G^C[GT]G
3243 CAGCTG
NcoI 3147 CCATGG C^CATGG
3574 CCATGG
NdeI 192 CATATG CA^TATG
4200 CATATG
PvuII 2305 CAGCTG CAG^CTG
3243 CAGCTG
SapI 1810 GAAGAGC GCTCTTC(1/4)
4173 GCTCTTC
SspI 198 AATATT AAT^ATT
301 AATATT
XmnI 2942 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2960 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII AvrII
BalI BanII BbvI BcnI BfaI
BglII BlnI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII