################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AclI AflII AgeI AscI AseI AsnI AspI AviII AvrII BamHI BclI BfrI BlnI BsaBI BspDI BssHII Bst1107I BstZ17I ClaI CpoI DraIII EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI MamI MluI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI SfiI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 3179 GACGTC GACGT^C Acc65I 825 GGTACC G^GTACC AcsI 1346 GAATTT [GA]^AATT[CT] AflIII 1584 ACATGT A^C[GA][CT]GT AhdI 617 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 233 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 4012 CCTGAGG CC^T[ACGT]AGG ApoI 1346 GAATTT [GA]^AATT[CT] Asp700I 415 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 825 GGTACC G^GTACC AspEI 617 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 67 GGCGCC GGCGC^C BbrPI 3230 CACGTG CAC^GTG BcgI 4136 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2271 GGATAC GTATCC(6/5) BsiWI 1528 CGTACG C^GTACG Bsp1407I 1275 TGTACA T^GTACA BspHI 1183 TCATGA T^CATGA BspLU11I 1584 ACATGT A^CATGT BsrGI 1275 TGTACA T^GTACA BstAPI 2636 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2795 TTCGAA TT^CGAA Bsu36I 4012 CCTGAGG CC^T[ACGT]AGG EagI 1515 CGGCCG C^GGCCG Eam1105I 617 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1515 CGGCCG C^GGCCG Eco52I 1515 CGGCCG C^GGCCG Eco81I 4012 CCTGAGG CC^T[ACGT]AGG KasI 67 GGCGCC G^GCGCC KpnI 825 GGTACC GGTAC^C MfeI 3862 CAATTG C^AATTG MunI 3862 CAATTG C^AATTG NaeI 1517 GCCGGC GCC^GGC NarI 67 GGCGCC GG^CGCC NdeI 147 CATATG CA^TATG NgoMIV 1517 GCCGGC G^CCGGC NspV 2795 TTCGAA TT^CGAA PmaCI 3230 CACGTG CAC^GTG PmlI 3230 CACGTG CAC^GTG RcaI 1183 TCATGA T^CATGA SacI 1226 GAGCTC GAGCT^C SexAI 2841 ACCAGGT A^CC[AT]GGT SfoI 67 GGCGCC GGC^GCC SfuI 2795 TTCGAA TT^CGAA SphI 3857 GCATGC GCATG^C SspBI 1275 TGTACA T^GTACA XcmI 346 CCACAGCGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 415 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 272 GTCTAC GT^[AC][GT]AC 1634 GTCTAC AccIII 15 TCCGGA T^CCGGA 198 TCCGGA Aor51HI 3168 AGCGCT AGC^GCT 3572 AGCGCT BlpI 1642 GCTGAGC GC^T[ACGT]AGC 3075 GCTGAGC Bpu1102I 1642 GCTGAGC GC^T[ACGT]AGC 3075 GCTGAGC BsaAI 1496 TACGTG [CT]AC^GT[GA] 3230 CACGTG BsaI 1439 GAGACC GGTCTC(1/5) 3689 GGTCTC BsaWI 15 TCCGGA [AT]^CCGG[AT] 198 TCCGGA BseAI 15 TCCGGA T^CCGGA 198 TCCGGA BsmI 141 GAATGC GAATGC(1/-1) 1250 GAATGC BspEI 15 TCCGGA T^CCGGA 198 TCCGGA BspMI 1869 ACCTGC ACCTGC(4/8) 1969 ACCTGC BsrDI 3003 CATTGC GCAATG(2/0) 4191 CATTGC BssSI 1 CTCGTG CACGAG(-5/-1) 2640 CTCGTG BstEII 1159 GGTCACC G^GT[ACGT]ACC 3130 GGTCACC BstPI 1159 GGTCACC G^GT[ACGT]ACC 3130 GGTCACC CelII 1642 GCTGAGC GC^T[ACGT]AGC 3075 GCTGAGC Eco47III 3168 AGCGCT AGC^GCT 3572 AGCGCT EcoO65I 1159 GGTCACC G^GT[ACGT]ACC 3130 GGTCACC HgaI 727 GACGC GACGC(5/10) 3613 GACGC HincII 59 GTCAAC GT[CT]^[GA]AC 1944 GTCAAC HindII 59 GTCAAC GT[CT]^[GA]AC 1944 GTCAAC KspI 3506 CCGCGG CCGC^GG 3539 CCGCGG MroI 15 TCCGGA T^CCGGA 198 TCCGGA NheI 1102 GCTAGC G^CTAGC 1691 GCTAGC NspI 1584 ACATGT [GA]CATG^[CT] 3857 GCATGC PstI 2855 CTGCAG CTGCA^G 3497 CTGCAG SacII 3506 CCGCGG CCGC^GG 3539 CCGCGG ScaI 42 AGTACT AGT^ACT 2709 AGTACT SmlI 722 CTCAAG C^T[CT][GA]AG 1922 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI