##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AclI        AflII       AgeI        AscI        AseI        
AsnI        AspI        AviII       AvrII       BamHI       
BclI        BfrI        BlnI        BsaBI       BspDI       
BssHII      Bst1107I    BstZ17I     ClaI        CpoI        
DraIII      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        MamI        
MluI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SalI        
SapI        SbfI        SfiI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII        3179  GACGTC               GACGT^C
Acc65I        825  GGTACC               G^GTACC
AcsI         1346  GAATTT               [GA]^AATT[CT]
AflIII       1584  ACATGT               A^C[GA][CT]GT
AhdI          617  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         233  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         4012  CCTGAGG              CC^T[ACGT]AGG
ApoI         1346  GAATTT               [GA]^AATT[CT]
Asp700I       415  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       825  GGTACC               G^GTACC
AspEI         617  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI           67  GGCGCC               GGCGC^C
BbrPI        3230  CACGTG               CAC^GTG
BcgI         4136  GCAGGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2271  GGATAC               GTATCC(6/5)
BsiWI        1528  CGTACG               C^GTACG
Bsp1407I     1275  TGTACA               T^GTACA
BspHI        1183  TCATGA               T^CATGA
BspLU11I     1584  ACATGT               A^CATGT
BsrGI        1275  TGTACA               T^GTACA
BstAPI       2636  GCATCTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2795  TTCGAA               TT^CGAA
Bsu36I       4012  CCTGAGG              CC^T[ACGT]AGG
EagI         1515  CGGCCG               C^GGCCG
Eam1105I      617  GACCCCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1515  CGGCCG               C^GGCCG
Eco52I       1515  CGGCCG               C^GGCCG
Eco81I       4012  CCTGAGG              CC^T[ACGT]AGG
KasI           67  GGCGCC               G^GCGCC
KpnI          825  GGTACC               GGTAC^C
MfeI         3862  CAATTG               C^AATTG
MunI         3862  CAATTG               C^AATTG
NaeI         1517  GCCGGC               GCC^GGC
NarI           67  GGCGCC               GG^CGCC
NdeI          147  CATATG               CA^TATG
NgoMIV       1517  GCCGGC               G^CCGGC
NspV         2795  TTCGAA               TT^CGAA
PmaCI        3230  CACGTG               CAC^GTG
PmlI         3230  CACGTG               CAC^GTG
RcaI         1183  TCATGA               T^CATGA
SacI         1226  GAGCTC               GAGCT^C
SexAI        2841  ACCAGGT              A^CC[AT]GGT
SfoI           67  GGCGCC               GGC^GCC
SfuI         2795  TTCGAA               TT^CGAA
SphI         3857  GCATGC               GCATG^C
SspBI        1275  TGTACA               T^GTACA
XcmI          346  CCACAGCGAGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          415  GAACGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI          272  GTCTAC               GT^[AC][GT]AC
             1634  GTCTAC              
AccIII         15  TCCGGA               T^CCGGA
              198  TCCGGA              
Aor51HI      3168  AGCGCT               AGC^GCT
             3572  AGCGCT              
BlpI         1642  GCTGAGC              GC^T[ACGT]AGC
             3075  GCTGAGC             
Bpu1102I     1642  GCTGAGC              GC^T[ACGT]AGC
             3075  GCTGAGC             
BsaAI        1496  TACGTG               [CT]AC^GT[GA]
             3230  CACGTG              
BsaI         1439  GAGACC               GGTCTC(1/5)
             3689  GGTCTC              
BsaWI          15  TCCGGA               [AT]^CCGG[AT]
              198  TCCGGA              
BseAI          15  TCCGGA               T^CCGGA
              198  TCCGGA              
BsmI          141  GAATGC               GAATGC(1/-1)
             1250  GAATGC              
BspEI          15  TCCGGA               T^CCGGA
              198  TCCGGA              
BspMI        1869  ACCTGC               ACCTGC(4/8)
             1969  ACCTGC              
BsrDI        3003  CATTGC               GCAATG(2/0)
             4191  CATTGC              
BssSI           1  CTCGTG               CACGAG(-5/-1)
             2640  CTCGTG              
BstEII       1159  GGTCACC              G^GT[ACGT]ACC
             3130  GGTCACC             
BstPI        1159  GGTCACC              G^GT[ACGT]ACC
             3130  GGTCACC             
CelII        1642  GCTGAGC              GC^T[ACGT]AGC
             3075  GCTGAGC             
Eco47III     3168  AGCGCT               AGC^GCT
             3572  AGCGCT              
EcoO65I      1159  GGTCACC              G^GT[ACGT]ACC
             3130  GGTCACC             
HgaI          727  GACGC                GACGC(5/10)
             3613  GACGC               
HincII         59  GTCAAC               GT[CT]^[GA]AC
             1944  GTCAAC              
HindII         59  GTCAAC               GT[CT]^[GA]AC
             1944  GTCAAC              
KspI         3506  CCGCGG               CCGC^GG
             3539  CCGCGG              
MroI           15  TCCGGA               T^CCGGA
              198  TCCGGA              
NheI         1102  GCTAGC               G^CTAGC
             1691  GCTAGC              
NspI         1584  ACATGT               [GA]CATG^[CT]
             3857  GCATGC              
PstI         2855  CTGCAG               CTGCA^G
             3497  CTGCAG              
SacII        3506  CCGCGG               CCGC^GG
             3539  CCGCGG              
ScaI           42  AGTACT               AGT^ACT
             2709  AGTACT              
SmlI          722  CTCAAG               C^T[CT][GA]AG
             1922  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        ApaI        ApaLI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BglII       BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BsrBI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        DraII       
DrdI        DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XhoII       
XmaCI       XmaI