##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI AspI AviII AvrII BamHI
BclI BfrI BlnI BsaBI BspDI
BssHII Bst1107I BstZ17I ClaI CpoI
DraIII EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI MamI
MluI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI SfiI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 3179 GACGTC GACGT^C
Acc65I 825 GGTACC G^GTACC
AcsI 1346 GAATTT [GA]^AATT[CT]
AflIII 1584 ACATGT A^C[GA][CT]GT
AhdI 617 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 233 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4012 CCTGAGG CC^T[ACGT]AGG
ApoI 1346 GAATTT [GA]^AATT[CT]
Asp700I 415 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 825 GGTACC G^GTACC
AspEI 617 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 67 GGCGCC GGCGC^C
BbrPI 3230 CACGTG CAC^GTG
BcgI 4136 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2271 GGATAC GTATCC(6/5)
BsiWI 1528 CGTACG C^GTACG
Bsp1407I 1275 TGTACA T^GTACA
BspHI 1183 TCATGA T^CATGA
BspLU11I 1584 ACATGT A^CATGT
BsrGI 1275 TGTACA T^GTACA
BstAPI 2636 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2795 TTCGAA TT^CGAA
Bsu36I 4012 CCTGAGG CC^T[ACGT]AGG
EagI 1515 CGGCCG C^GGCCG
Eam1105I 617 GACCCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1515 CGGCCG C^GGCCG
Eco52I 1515 CGGCCG C^GGCCG
Eco81I 4012 CCTGAGG CC^T[ACGT]AGG
KasI 67 GGCGCC G^GCGCC
KpnI 825 GGTACC GGTAC^C
MfeI 3862 CAATTG C^AATTG
MunI 3862 CAATTG C^AATTG
NaeI 1517 GCCGGC GCC^GGC
NarI 67 GGCGCC GG^CGCC
NdeI 147 CATATG CA^TATG
NgoMIV 1517 GCCGGC G^CCGGC
NspV 2795 TTCGAA TT^CGAA
PmaCI 3230 CACGTG CAC^GTG
PmlI 3230 CACGTG CAC^GTG
RcaI 1183 TCATGA T^CATGA
SacI 1226 GAGCTC GAGCT^C
SexAI 2841 ACCAGGT A^CC[AT]GGT
SfoI 67 GGCGCC GGC^GCC
SfuI 2795 TTCGAA TT^CGAA
SphI 3857 GCATGC GCATG^C
SspBI 1275 TGTACA T^GTACA
XcmI 346 CCACAGCGAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 415 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 272 GTCTAC GT^[AC][GT]AC
1634 GTCTAC
AccIII 15 TCCGGA T^CCGGA
198 TCCGGA
Aor51HI 3168 AGCGCT AGC^GCT
3572 AGCGCT
BlpI 1642 GCTGAGC GC^T[ACGT]AGC
3075 GCTGAGC
Bpu1102I 1642 GCTGAGC GC^T[ACGT]AGC
3075 GCTGAGC
BsaAI 1496 TACGTG [CT]AC^GT[GA]
3230 CACGTG
BsaI 1439 GAGACC GGTCTC(1/5)
3689 GGTCTC
BsaWI 15 TCCGGA [AT]^CCGG[AT]
198 TCCGGA
BseAI 15 TCCGGA T^CCGGA
198 TCCGGA
BsmI 141 GAATGC GAATGC(1/-1)
1250 GAATGC
BspEI 15 TCCGGA T^CCGGA
198 TCCGGA
BspMI 1869 ACCTGC ACCTGC(4/8)
1969 ACCTGC
BsrDI 3003 CATTGC GCAATG(2/0)
4191 CATTGC
BssSI 1 CTCGTG CACGAG(-5/-1)
2640 CTCGTG
BstEII 1159 GGTCACC G^GT[ACGT]ACC
3130 GGTCACC
BstPI 1159 GGTCACC G^GT[ACGT]ACC
3130 GGTCACC
CelII 1642 GCTGAGC GC^T[ACGT]AGC
3075 GCTGAGC
Eco47III 3168 AGCGCT AGC^GCT
3572 AGCGCT
EcoO65I 1159 GGTCACC G^GT[ACGT]ACC
3130 GGTCACC
HgaI 727 GACGC GACGC(5/10)
3613 GACGC
HincII 59 GTCAAC GT[CT]^[GA]AC
1944 GTCAAC
HindII 59 GTCAAC GT[CT]^[GA]AC
1944 GTCAAC
KspI 3506 CCGCGG CCGC^GG
3539 CCGCGG
MroI 15 TCCGGA T^CCGGA
198 TCCGGA
NheI 1102 GCTAGC G^CTAGC
1691 GCTAGC
NspI 1584 ACATGT [GA]CATG^[CT]
3857 GCATGC
PstI 2855 CTGCAG CTGCA^G
3497 CTGCAG
SacII 3506 CCGCGG CCGC^GG
3539 CCGCGG
ScaI 42 AGTACT AGT^ACT
2709 AGTACT
SmlI 722 CTCAAG C^T[CT][GA]AG
1922 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmaCI XmaI