##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AviII BamHI BbrPI BcgI BciVI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HindIII KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 4551 GTCTAC GT^[AC][GT]AC
AccII 4679 CGCG CG^CG
AcyI 3423 GGCGCC G[GA]^CG[CT]C
AflII 952 CTTAAG C^TTAAG
AhdI 3452 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3452 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 426 CTCGGG C^[CT]CG[GA]G
AvrII 289 CCTAGG C^CTAGG
BbeI 3423 GGCGCC GGCGC^C
BclI 938 TGATCA T^GATCA
BfrI 952 CTTAAG C^TTAAG
BglII 4 AGATCT A^GATCT
BlnI 289 CCTAGG C^CTAGG
BsaAI 4351 CACGTA [CT]AC^GT[GA]
BsaHI 3423 GGCGCC G[GA]^CG[CT]C
BsmBI 1198 GAGACG CGTCTC(1/5)
BsmI 501 GAATGC GAATGC(1/-1)
BsoBI 426 CTCGGG C^[CT]CG[GA]G
Bsp1407I 471 TGTACA T^GTACA
BsrGI 471 TGTACA T^GTACA
BstUI 4679 CGCG CG^CG
CfoI 3424 GCGC GCG^C
DrdI 478 GACACCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3452 GACCTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 352 GAAGAG CTCTTC(1/4)
EcoRI 3311 GAATTC G^AATTC
FbaI 938 TGATCA T^GATCA
HaeII 3423 GGCGCC [GA]GCGC^[CT]
HhaI 3424 GCGC GCG^C
Hin1I 3423 GGCGCC G[GA]^CG[CT]C
HinP1I 3424 GCGC G^CGC
HpaI 4210 GTTAAC GTT^AAC
KasI 3423 GGCGCC G^GCGCC
Ksp632I 352 GAAGAG CTCTTC(1/4)
MvnI 4679 CGCG CG^CG
NaeI 2107 GCCGGC GCC^GGC
NarI 3423 GGCGCC GG^CGCC
NgoMIV 2107 GCCGGC G^CCGGC
SfoI 3423 GGCGCC GGC^GCC
SphI 3239 GCATGC GCATG^C
SspBI 471 TGTACA T^GTACA
XbaI 2199 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 3519 ACATGT A^C[GA][CT]GT
3552 ACATGT
AseI 3513 ATTAAT AT^TAAT
4045 ATTAAT
AsnI 3513 ATTAAT AT^TAAT
4045 ATTAAT
AspI 1107 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4714 GACTCTGTC
BglI 2480 GCCCCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4530 GCCAACATGGC
BspLU11I 3519 ACATGT A^CATGT
3552 ACATGT
BsrFI 1917 ACCGGC [GA]^CCGG[CT]
2107 GCCGGC
BssSI 407 CACGAG CACGAG(-5/-1)
4630 CACGAG
BstAPI 1768 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2417 GCACTGTCTGC
Cfr10I 1917 ACCGGC [GA]^CCGG[CT]
2107 GCCGGC
DraI 3229 TTTAAA TTT^AAA
4565 TTTAAA
HgaI 4056 GCGTC GACGC(5/10)
4680 GCGTC
HincII 521 GTTGAC GT[CT]^[GA]AC
4210 GTTAAC
HindII 521 GTTGAC GT[CT]^[GA]AC
4210 GTTAAC
MaeII 2212 ACGT A^CGT
4352 ACGT
NheI 187 GCTAGC G^CTAGC
602 GCTAGC
PflFI 1107 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4714 GACTCTGTC
PshBI 3513 ATTAAT AT^TAAT
4045 ATTAAT
SacI 1541 GAGCTC GAGCT^C
3613 GAGCTC
Tth111I 1107 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4714 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI