##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BciVI BsaBI
BsmI Bsp1407I BspDI BspHI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI Eam1105I
EcoO65I EcoRI EcoRV FspI HincII
HindII HpaI KpnI KspI MamI
NdeI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 3743 TCCGGA T^CCGGA
AcsI 805 AAATTC [GA]^AATT[CT]
AflII 177 CTTAAG C^TTAAG
ApoI 805 AAATTC [GA]^AATT[CT]
Asp700I 2783 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1662 CCTAGG C^CTAGG
BbrPI 2086 CACGTG CAC^GTG
BcgI 2261 GCAAGAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 177 CTTAAG C^TTAAG
BglII 1859 AGATCT A^GATCT
BlnI 1662 CCTAGG C^CTAGG
BsaAI 2086 CACGTG [CT]AC^GT[GA]
BsaWI 3743 TCCGGA [AT]^CCGG[AT]
BseAI 3743 TCCGGA T^CCGGA
BsiEI 2504 CGGCCG CG[GA][CT]^CG
BsiWI 1980 CGTACG C^GTACG
BspEI 3743 TCCGGA T^CCGGA
BspLU11I 2345 ACATGT A^CATGT
BsrDI 2028 CATTGC GCAATG(2/0)
BssSI 101 CTCGTG CACGAG(-5/-1)
EagI 2504 CGGCCG C^GGCCG
EclXI 2504 CGGCCG C^GGCCG
Eco52I 2504 CGGCCG C^GGCCG
EcoT22I 535 ATGCAT ATGCA^T
FseI 4173 GGCCGGCC GGCCGG^CC
HindIII 3738 AAGCTT A^AGCTT
MfeI 3668 CAATTG C^AATTG
MluI 3401 ACGCGT A^CGCGT
MroI 3743 TCCGGA T^CCGGA
MunI 3668 CAATTG C^AATTG
NaeI 4174 GCCGGC GCC^GGC
NgoMIV 4174 GCCGGC G^CCGGC
NheI 568 GCTAGC G^CTAGC
NsiI 535 ATGCAT ATGCA^T
PmaCI 2086 CACGTG CAC^GTG
PmlI 2086 CACGTG CAC^GTG
SacI 3820 GAGCTC GAGCT^C
SapI 2694 GCTCTTC GCTCTTC(1/4)
SbfI 1011 CCTGCAGG CCTGCA^GG
SexAI 4026 ACCTGGT A^CC[AT]GGT
SfiI 4177 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3104 CCCGGG CCC^GGG
SphI 753 GCATGC GCATG^C
Sse8387I 1011 CCTGCAGG CCTGCA^GG
XmaCI 3104 CCCGGG C^CCGGG
XmaI 3104 CCCGGG C^CCGGG
XmnI 2783 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2746 GTCTAC GT^[AC][GT]AC
3919 GTCTAC
Alw44I 345 GTGCAC G^TGCAC
1086 GTGCAC
Aor51HI 1970 AGCGCT AGC^GCT
2846 AGCGCT
ApaI 3472 GGGCCC GGGCC^C
3834 GGGCCC
ApaLI 345 GTGCAC G^TGCAC
1086 GTGCAC
BbeI 2005 GGCGCC GGCGC^C
2767 GGCGCC
BclI 420 TGATCA T^GATCA
1557 TGATCA
BsrBI 1730 CCGCTC CCGCTC(-3/-3)
694 GAGCGG
DrdI 2041 GACGGCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2449 GACGTGGCCGTC
Eco47III 1970 AGCGCT AGC^GCT
2846 AGCGCT
FbaI 420 TGATCA T^GATCA
1557 TGATCA
KasI 2005 GGCGCC G^GCGCC
2767 GGCGCC
NarI 2005 GGCGCC GG^CGCC
2767 GGCGCC
NcoI 3815 CCATGG C^CATGG
4245 CCATGG
PflMI 732 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4024 CCACCTGGTGG
SfoI 2005 GGCGCC GGC^GCC
2767 GGCGCC
StuI 3538 AGGCCT AGG^CCT
3891 AGGCCT
Van91I 732 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4024 CCACCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII