##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI AocI AspEI BbeI
BcgI BclI BfrI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspDI BspLU11I
BsrGI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII ClaI Eam1105I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
HincII HindII HpaI KasI MamI
MfeI MluI MunI NarI NdeI
NotI NruI NsiI PacI PinAI
PmeI Psp1406I PstI PvuI SacI
SalI SapI SbfI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 1978 GGTACC G^GTACC
AcyI 293 GGCGTC G[GA]^CG[CT]C
Alw44I 365 GTGCAC G^TGCAC
ApaLI 365 GTGCAC G^TGCAC
AscI 591 GGCGCGCC GG^CGCGCC
AseI 743 ATTAAT AT^TAAT
AsnI 743 ATTAAT AT^TAAT
Asp718I 1978 GGTACC G^GTACC
AviII 3512 TGCGCA TGC^GCA
AvrII 1573 CCTAGG C^CTAGG
BamHI 227 GGATCC G^GATCC
BbrPI 3632 CACGTG CAC^GTG
BciVI 806 GTATCC GTATCC(6/5)
BlnI 1573 CCTAGG C^CTAGG
BsaHI 293 GGCGTC G[GA]^CG[CT]C
BsaI 1945 GGTCTC GGTCTC(1/5)
BsiEI 157 CGGCCG CG[GA][CT]^CG
BsmI 1745 GCATTC GAATGC(1/-1)
BsrBI 322 CCGCTC CCGCTC(-3/-3)
BssHII 592 GCGCGC G^CGCGC
BssSI 443 CACGAG CACGAG(-5/-1)
BstBI 697 TTCGAA TT^CGAA
CpoI 4030 CGGACCG CG^G[AT]CCG
DraI 1303 TTTAAA TTT^AAA
DraIII 2160 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1830 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 157 CGGCCG C^GGCCG
EclXI 157 CGGCCG C^GGCCG
Eco52I 157 CGGCCG C^GGCCG
EcoNI 4462 CCTATCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2711 GATATC GAT^ATC
FspI 3512 TGCGCA TGC^GCA
Hin1I 293 GGCGTC G[GA]^CG[CT]C
HindIII 879 AAGCTT A^AGCTT
KpnI 1978 GGTACC GGTAC^C
KspI 272 CCGCGG CCGC^GG
MslI 368 CACTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 3727 CCATGG C^CATGG
NheI 937 GCTAGC G^CTAGC
NspV 697 TTCGAA TT^CGAA
PaeR7I 2042 CTCGAG C^TCGAG
PmaCI 3632 CACGTG CAC^GTG
PmlI 3632 CACGTG CAC^GTG
PshAI 4418 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 743 ATTAAT AT^TAAT
RsrII 4030 CGGACCG CG^G[AT]CCG
SacII 272 CCGCGG CCGC^GG
SexAI 2643 ACCAGGT A^CC[AT]GGT
SfcI 212 CTACAG C^T[GA][CT]AG
SfuI 697 TTCGAA TT^CGAA
SmaI 603 CCCGGG CCC^GGG
SphI 359 GCATGC GCATG^C
SspI 47 AATATT AAT^ATT
XhoI 2042 CTCGAG C^TCGAG
XmaCI 603 CCCGGG C^CCGGG
XmaI 603 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccIII 3414 TCCGGA T^CCGGA
4028 TCCGGA
AspI 1940 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACGGTGTC
BbvCI 2151 CCTCAGC CCTCAGC(-5/-2)
3757 GCTGAGG
BsaAI 372 TACGTG [CT]AC^GT[GA]
3632 CACGTG
BseAI 3414 TCCGGA T^CCGGA
4028 TCCGGA
BsmBI 4198 CGTCTC CGTCTC(1/5)
1508 GAGACG
BspEI 3414 TCCGGA T^CCGGA
4028 TCCGGA
BspHI 1757 TCATGA T^CATGA
1806 TCATGA
BspMI 4090 ACCTGC ACCTGC(4/8)
3139 GCAGGT
MroI 3414 TCCGGA T^CCGGA
4028 TCCGGA
NspI 359 GCATGC [GA]CATG^[CT]
2804 ACATGC
PflFI 1940 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACGGTGTC
PflMI 2802 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3727 CCATGGCCTGG
RcaI 1757 TCATGA T^CATGA
1806 TCATGA
ScaI 1116 AGTACT AGT^ACT
2975 AGTACT
Tth111I 1940 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACGGTGTC
Van91I 2802 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3727 CCATGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI Aor51HI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI