##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AgeI AhdI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BlnI BsiWI BsmBI
Bsp1407I BspDI BspHI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI Eam1105I FseI FspI HindIII
HpaI KpnI KspI MfeI MunI
NdeI NheI NotI NspV PacI
PinAI PmeI PshBI RcaI RsrII
SacI SacII SalI SexAI SfiI
SfuI SnaBI SphI SspBI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1314 GTAGAC GT^[AC][GT]AC
AclI 1483 AACGTT AA^CGTT
AflII 2882 CTTAAG C^TTAAG
Alw44I 2832 GTGCAC G^TGCAC
Aor51HI 800 AGCGCT AGC^GCT
ApaLI 2832 GTGCAC G^TGCAC
AspI 822 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 2301 TGATCA T^GATCA
BfrI 2882 CTTAAG C^TTAAG
BglII 526 AGATCT A^GATCT
BsaBI 1079 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 715 CATTGC GCAATG(2/0)
DraIII 4070 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2091 CGGCCG C^GGCCG
EclXI 2091 CGGCCG C^GGCCG
Eco47III 800 AGCGCT AGC^GCT
Eco52I 2091 CGGCCG C^GGCCG
EcoRV 3487 GATATC GAT^ATC
EcoT22I 2948 ATGCAT ATGCA^T
FbaI 2301 TGATCA T^GATCA
MamI 1079 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 108 ACGCGT A^CGCGT
NaeI 2551 GCCGGC GCC^GGC
NgoMIV 2551 GCCGGC G^CCGGC
NruI 1210 TCGCGA TCG^CGA
NsiI 2948 ATGCAT ATGCA^T
PaeR7I 1591 CTCGAG C^TCGAG
PflFI 822 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 331 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 629 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1483 AACGTT AA^CGTT
PvuI 4143 CGATCG CGAT^CG
SbfI 1706 CCTGCAGG CCTGCA^GG
ScaI 654 AGTACT AGT^ACT
SgrAI 2102 CACCGGCG C[GA]^CCGG[CT]G
SmaI 61 CCCGGG CCC^GGG
SpeI 959 ACTAGT A^CTAGT
Sse8387I 1706 CCTGCAGG CCTGCA^GG
SspI 4780 AATATT AAT^ATT
StuI 2745 AGGCCT AGG^CCT
Tth111I 822 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 331 CCAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1591 CTCGAG C^TCGAG
XmaCI 61 CCCGGG C^CCGGG
XmaI 61 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 2157 TCCGGA T^CCGGA
2229 TCCGGA
AocI 1037 CCTGAGG CC^T[ACGT]AGG
1665 CCTGAGG
Asp700I 347 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
983 GAATTCTTTC
BcgI 4273 CGACGCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1583 GCATGGCCCTCG
BciVI 2267 GGATAC GTATCC(6/5)
2359 GTATCC
BlpI 171 GCTAAGC GC^T[ACGT]AGC
3034 GCTCAGC
Bpu1102I 171 GCTAAGC GC^T[ACGT]AGC
3034 GCTCAGC
BseAI 2157 TCCGGA T^CCGGA
2229 TCCGGA
BspEI 2157 TCCGGA T^CCGGA
2229 TCCGGA
BspLU11I 547 ACATGT A^CATGT
757 ACATGT
BsrBI 1922 CCGCTC CCGCTC(-3/-3)
3032 CCGCTC
BstAPI 4506 GCAGGGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4533 GCATTTTGTGC
BstEII 1892 GGTCACC G^GT[ACGT]ACC
4119 GGTAACC
BstPI 1892 GGTCACC G^GT[ACGT]ACC
4119 GGTAACC
BstYI 526 AGATCT [GA]^GATC[CT]
4478 GGATCT
Bsu36I 1037 CCTGAGG CC^T[ACGT]AGG
1665 CCTGAGG
CelII 171 GCTAAGC GC^T[ACGT]AGC
3034 GCTCAGC
DraI 1127 TTTAAA TTT^AAA
1142 TTTAAA
DrdI 474 GACCTCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4030 GACCGACCAGTC
Eco81I 1037 CCTGAGG CC^T[ACGT]AGG
1665 CCTGAGG
EcoNI 2449 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2491 CCTCTTCAAGG
EcoO65I 1892 GGTCACC G^GT[ACGT]ACC
4119 GGTAACC
EcoRI 983 GAATTC G^AATTC
1450 GAATTC
HincII 3990 GTCAAC GT[CT]^[GA]AC
4788 GTCAAC
HindII 3990 GTCAAC GT[CT]^[GA]AC
4788 GTCAAC
MflI 526 AGATCT [GA]^GATC[CT]
4478 GGATCT
MroI 2157 TCCGGA T^CCGGA
2229 TCCGGA
NcoI 128 CCATGG C^CATGG
1607 CCATGG
NspI 547 ACATGT [GA]CATG^[CT]
757 ACATGT
SapI 1029 GAAGAGC GCTCTTC(1/4)
3882 GCTCTTC
XhoII 526 AGATCT [GA]^GATC[CT]
4478 GGATCT
XmnI 347 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
983 GAATTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI