##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI BalI BbeI BbrPI BcgI
BclI BglI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I CelII Cfr10I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FbaI FseI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
Sse8387I StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 3190 GGTACC G^GTACC
AflII 3547 CTTAAG C^TTAAG
Asp718I 3190 GGTACC G^GTACC
AviII 2456 TGCGCA TGC^GCA
BamHI 53 GGATCC G^GATCC
BbvCI 3844 CCTCAGC CCTCAGC(-5/-2)
BciVI 1287 GGATAC GTATCC(6/5)
BfrI 3547 CTTAAG C^TTAAG
BglII 1492 AGATCT A^GATCT
Bsp1407I 4730 TGTACA T^GTACA
BspDI 749 ATCGAT AT^CGAT
BsrGI 4730 TGTACA T^GTACA
BstBI 1898 TTCGAA TT^CGAA
CfoI 2457 GCGC GCG^C
ClaI 749 ATCGAT AT^CGAT
EaeI 4056 CGGCCA [CT]^GGCC[GA]
EcoNI 1877 CCTCTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1219 GATATC GAT^ATC
FauI 59 CCCGC CCCGC(4/6)
FspI 2456 TGCGCA TGC^GCA
HgaI 2042 GCGTC GACGC(5/10)
HhaI 2457 GCGC GCG^C
HinP1I 2457 GCGC G^CGC
KpnI 3190 GGTACC GGTAC^C
NcoI 4475 CCATGG C^CATGG
NheI 2224 GCTAGC G^CTAGC
NspV 1898 TTCGAA TT^CGAA
PflMI 840 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 3230 GAAGAGC GCTCTTC(1/4)
ScaI 4723 AGTACT AGT^ACT
SexAI 1072 ACCTGGT A^CC[AT]GGT
SfuI 1898 TTCGAA TT^CGAA
SmaI 3821 CCCGGG CCC^GGG
SspBI 4730 TGTACA T^GTACA
Van91I 840 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 124 CCAAAGCTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3821 CCCGGG C^CCGGG
XmaI 3821 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 339 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1839 CAGATGCTG
AocI 1848 CCTGAGG CC^T[ACGT]AGG
3954 CCTCAGG
ApaI 674 GGGCCC GGGCC^C
1952 GGGCCC
Asp700I 240 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2126 GAAGAATTTC
AspHI 1707 GAGCAC G[AT]GC[AT]^C
3233 GAGCAC
AvaI 2014 CCCGAG C^[CT]CG[GA]G
3821 CCCGGG
BsiHKAI 1707 GAGCAC G[AT]GC[AT]^C
3233 GAGCAC
BsmBI 2043 CGTCTC CGTCTC(1/5)
3908 GAGACG
BsmI 2453 GAATGC GAATGC(1/-1)
2468 GCATTC
BsoBI 2014 CCCGAG C^[CT]CG[GA]G
3821 CCCGGG
BstXI 3854 CCAGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3982 CCAAAGTGCTGG
Bsu36I 1848 CCTGAGG CC^T[ACGT]AGG
3954 CCTCAGG
DrdI 1475 GACCAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4651 GACACTAGGGTC
Eco81I 1848 CCTGAGG CC^T[ACGT]AGG
3954 CCTCAGG
EcoRI 1869 GAATTC G^AATTC
2101 GAATTC
EcoT22I 3629 ATGCAT ATGCA^T
4368 ATGCAT
MspA1I 258 CAGCTG C[AC]G^C[GT]G
1338 CAGCTG
NsiI 3629 ATGCAT ATGCA^T
4368 ATGCAT
PvuII 258 CAGCTG CAG^CTG
1338 CAGCTG
SpeI 1389 ACTAGT A^CTAGT
2735 ACTAGT
SphI 3874 GCATGC GCATG^C
4370 GCATGC
XbaI 619 TCTAGA T^CTAGA
2642 TCTAGA
XmnI 240 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2126 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII AvrII BanI
BanII BbsI BbvI BcnI BfaI
BlnI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshAI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII