##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AseI AsnI Asp700I AspI AvrII
BamHI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BspEI CpoI DraI
EcoT22I FseI HpaI MamI MluI
MroI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RsrII SalI SgrAI
SnaBI SpeI SspI SwaI TfiI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 1792 GGTACC G^GTACC
AscI 5350 GGCGCGCC GG^CGCGCC
Asp718I 1792 GGTACC G^GTACC
BclI 7749 TGATCA T^GATCA
BglII 3739 AGATCT A^GATCT
BspDI 5996 ATCGAT AT^CGAT
BspHI 1191 TCATGA T^CATGA
BspLU11I 3150 ACATGT A^CATGT
Bst1107I 5855 GTATAC GTA^TAC
BstBI 5192 TTCGAA TT^CGAA
BstZ17I 5855 GTATAC GTA^TAC
ClaI 5996 ATCGAT AT^CGAT
DrdI 3344 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 6235 GATATC GAT^ATC
FbaI 7749 TGATCA T^GATCA
HincII 349 GTTGAC GT[CT]^[GA]AC
HindII 349 GTTGAC GT[CT]^[GA]AC
HindIII 6445 AAGCTT A^AGCTT
KpnI 1792 GGTACC GGTAC^C
MfeI 1009 CAATTG C^AATTG
MunI 1009 CAATTG C^AATTG
NspV 5192 TTCGAA TT^CGAA
PvuI 3547 CGATCG CGAT^CG
RcaI 1191 TCATGA T^CATGA
ScaI 6249 AGTACT AGT^ACT
SfiI 8328 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 5192 TTCGAA TT^CGAA
XbaI 769 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 554 GAATTC [GA]^AATT[CT]
6671 GAATTC
Alw44I 1987 GTGCAC G^TGCAC
2482 GTGCAC
ApaLI 1987 GTGCAC G^TGCAC
2482 GTGCAC
ApoI 554 GAATTC [GA]^AATT[CT]
6671 GAATTC
BciVI 1861 GGATAC GTATCC(6/5)
2201 GGATAC
Bsp1407I 1801 TGTACA T^GTACA
1911 TGTACA
BsrGI 1801 TGTACA T^GTACA
1911 TGTACA
EcoRI 554 GAATTC G^AATTC
6671 GAATTC
KspI 4202 CCGCGG CCGC^GG
7768 CCGCGG
MseI 870 TTAA T^TAA
1224 TTAA
NheI 1534 GCTAGC G^CTAGC
7180 GCTAGC
PshAI 3794 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6043 GACCCCAGTC
SacII 4202 CCGCGG CCGC^GG
7768 CCGCGG
SexAI 2100 ACCTGGT A^CC[AT]GGT
6117 ACCAGGT
SspBI 1801 TGTACA T^GTACA
1911 TGTACA
Tru9I 870 TTAA T^TAA
1224 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 166 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI Aor51HI ApaI AspEI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfoI SmaI
SmlI SphI Sse8387I StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI