##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI Aor51HI AscI AspEI AviII
AvrII BamHI BbeI BfrI BlnI
BseAI BsiEI BsiWI BspEI BspHI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I FseI FspI
HpaI KasI MluI MroI NarI
NheI NotI NruI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SbfI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 4883 GGTACC G^GTACC
AseI 7405 ATTAAT AT^TAAT
AsnI 7405 ATTAAT AT^TAAT
Asp718I 4883 GGTACC G^GTACC
BclI 5835 TGATCA T^GATCA
Bsp1407I 685 TGTACA T^GTACA
BspDI 1887 ATCGAT AT^CGAT
BsrGI 685 TGTACA T^GTACA
ClaI 1887 ATCGAT AT^CGAT
DraI 7239 TTTAAA TTT^AAA
EcoRV 5029 GATATC GAT^ATC
FbaI 5835 TGATCA T^GATCA
HaeII 4226 AGCGCC [GA]GCGC^[CT]
HgaI 6454 GACGC GACGC(5/10)
KpnI 4883 GGTACC GGTAC^C
NaeI 3911 GCCGGC GCC^GGC
NdeI 3086 CATATG CA^TATG
NgoMIV 3911 GCCGGC G^CCGGC
PaeR7I 6920 CTCGAG C^TCGAG
PshBI 7405 ATTAAT AT^TAAT
SacI 5500 GAGCTC GAGCT^C
SalI 6466 GTCGAC G^TCGAC
SexAI 7159 ACCAGGT A^CC[AT]GGT
SfiI 206 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3549 GCATGC GCATG^C
SspBI 685 TGTACA T^GTACA
SspI 3062 AATATT AAT^ATT
XhoI 6920 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3291 GACGTC GACGT^C
3488 GACGTC
AccII 4690 CGCG CG^CG
6964 CGCG
Asp700I 1146 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3037 GAAAAGCTTC
BbrPI 4393 CACGTG CAC^GTG
6099 CACGTG
BbvCI 2379 CCTCAGC CCTCAGC(-5/-2)
7472 CCTCAGC
BlpI 6635 GCTCAGC GC^T[ACGT]AGC
6914 GCTCAGC
Bpu1102I 6635 GCTCAGC GC^T[ACGT]AGC
6914 GCTCAGC
BsmBI 4977 CGTCTC CGTCTC(1/5)
2343 GAGACG
BspLU11I 1256 ACATGT A^CATGT
3141 ACATGT
BsrFI 3911 GCCGGC [GA]^CCGG[CT]
5872 ACCGGC
BstEII 249 GGTCACC G^GT[ACGT]ACC
5455 GGTGACC
BstPI 249 GGTCACC G^GT[ACGT]ACC
5455 GGTGACC
BstUI 4690 CGCG CG^CG
6964 CGCG
BstXI 6690 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7304 CCATCATCCTGG
CelII 6635 GCTCAGC GC^T[ACGT]AGC
6914 GCTCAGC
CfoI 36 GCGC GCG^C
4227 GCGC
Cfr10I 3911 GCCGGC [GA]^CCGG[CT]
5872 ACCGGC
DrdI 1272 GACTGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6948 GACTGACCTGTC
EcoO65I 249 GGTCACC G^GT[ACGT]ACC
5455 GGTGACC
EcoRI 5120 GAATTC G^AATTC
7616 GAATTC
HhaI 36 GCGC GCG^C
4227 GCGC
HinP1I 36 GCGC G^CGC
4227 GCGC
HincII 6466 GTCGAC GT[CT]^[GA]AC
7944 GTCAAC
HindII 6466 GTCGAC GT[CT]^[GA]AC
7944 GTCAAC
KspI 4689 CCGCGG CCGC^GG
6963 CCGCGG
MfeI 3204 CAATTG C^AATTG
3393 CAATTG
MunI 3204 CAATTG C^AATTG
3393 CAATTG
MvnI 4690 CGCG CG^CG
6964 CGCG
PmaCI 4393 CACGTG CAC^GTG
6099 CACGTG
PmlI 4393 CACGTG CAC^GTG
6099 CACGTG
SacII 4689 CCGCGG CCGC^GG
6963 CCGCGG
ScaI 5038 AGTACT AGT^ACT
5840 AGTACT
SmaI 2696 CCCGGG CCC^GGG
6339 CCCGGG
XbaI 3478 TCTAGA T^CTAGA
6936 TCTAGA
XmaCI 2696 CCCGGG C^CCGGG
6339 CCCGGG
XmaI 2696 CCCGGG C^CCGGG
6339 CCCGGG
XmnI 1146 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3037 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcgI BciVI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
Hin1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII