##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AscI AseI AsnI AviII
AvrII BamHI BbeI BbrPI BclI
BfrI BlnI BsaAI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII EcoRV EcoT22I FbaI FspI
HpaI KasI KspI MamI MfeI
MluI MroI MslI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI TfiI
XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1066 GGTACC G^GTACC
AcsI 2398 GAATTC [GA]^AATT[CT]
Alw44I 1526 GTGCAC G^TGCAC
Aor51HI 2649 AGCGCT AGC^GCT
ApaLI 1526 GTGCAC G^TGCAC
ApoI 2398 GAATTC [GA]^AATT[CT]
Asp700I 1180 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1066 GGTACC G^GTACC
BcgI 675 GCAGCTATTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 286 AGATCT A^GATCT
BsmI 1454 GAATGC GAATGC(1/-1)
Bsp1407I 827 TGTACA T^GTACA
BsrGI 827 TGTACA T^GTACA
BstXI 833 CCAACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1161 GACCCTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2649 AGCGCT AGC^GCT
EcoNI 1003 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2398 GAATTC G^AATTC
EcoT14I 148 CCATGG C^C[AT][AT]GG
FseI 2301 GGCCGGCC GGCCGG^CC
HindIII 2851 AAGCTT A^AGCTT
KpnI 1066 GGTACC GGTAC^C
NcoI 148 CCATGG C^CATGG
NspI 1348 GCATGT [GA]CATG^[CT]
PshAI 1013 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2866 GAAGAGC GCTCTTC(1/4)
SbfI 1505 CCTGCAGG CCTGCA^GG
SmaI 1393 CCCGGG CCC^GGG
Sse8387I 1505 CCTGCAGG CCTGCA^GG
SspBI 827 TGTACA T^GTACA
StuI 2339 AGGCCT AGG^CCT
StyI 148 CCATGG C^C[AT][AT]GG
XmaCI 1393 CCCGGG C^CCGGG
XmaI 1393 CCCGGG C^CCGGG
XmnI 1180 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 2196 CGCG CG^CG
2640 CGCG
AhdI 377 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACACAAGGTC
AlwI 866 GATCC GGATC(4/5)
1585 GGATC
AspEI 377 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACACAAGGTC
AspI 703 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2464 GACTTAGTC
BbvCI 2342 CCTCAGC CCTCAGC(-5/-2)
2558 CCTCAGC
BstAPI 2519 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2787 GCATCTGCTGC
BstEII 1541 GGTCACC G^GT[ACGT]ACC
1886 GGTCACC
BstPI 1541 GGTCACC G^GT[ACGT]ACC
1886 GGTCACC
BstUI 2196 CGCG CG^CG
2640 CGCG
EagI 2300 CGGCCG C^GGCCG
2304 CGGCCG
Eam1105I 377 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1753 GACACAAGGTC
EarI 2552 CTCTTC CTCTTC(1/4)
2866 GAAGAG
EclXI 2300 CGGCCG C^GGCCG
2304 CGGCCG
Eco52I 2300 CGGCCG C^GGCCG
2304 CGGCCG
EcoO65I 1541 GGTCACC G^GT[ACGT]ACC
1886 GGTCACC
HincII 619 GTCAAC GT[CT]^[GA]AC
814 GTCAAC
HindII 619 GTCAAC GT[CT]^[GA]AC
814 GTCAAC
Ksp632I 2552 CTCTTC CTCTTC(1/4)
2866 GAAGAG
MseI 2506 TTAA T^TAA
2848 TTAA
MvnI 2196 CGCG CG^CG
2640 CGCG
PaeR7I 1165 CTCGAG C^TCGAG
1678 CTCGAG
PflFI 703 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2464 GACTTAGTC
PvuII 895 CAGCTG CAG^CTG
935 CAGCTG
SmlI 1165 CTCGAG C^T[CT][GA]AG
1678 CTCGAG
Tru9I 2506 TTAA T^TAA
2848 TTAA
Tsp509I 2399 AATT ^AATT
2846 AATT
Tth111I 703 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2464 GACTTAGTC
XcmI 464 CCAGGGCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1032 CCAGCCAGTGGCTGG
XhoI 1165 CTCGAG C^TCGAG
1678 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AatII AciI AcyI AfaI AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XhoII