##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI Aor51HI ApaI AscI Asp718I
AvrII BbeI BbrPI BbvCI BcgI
BciVI BfrI BlnI BsaHI BsiWI
BspDI BspLU11I BssHII Bst1107I BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRV FseI
Hin1I KasI KpnI MluI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I RsrII SalI SbfI SfoI
SgrAI SnaBI SpeI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflIII 1743 ACACGT A^C[GA][CT]GT
AhdI 4223 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 6021 ATTAAT AT^TAAT
AsnI 6021 ATTAAT AT^TAAT
AspEI 4223 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4253 TGCGCA TGC^GCA
BglI 4281 GCCTGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 4213 GGTCTC GGTCTC(1/5)
BsrDI 885 GCAATG GCAATG(2/0)
BstBI 3590 TTCGAA TT^CGAA
DraII 1605 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 4223 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1605 AGGACCT [GA]G^G[ACGT]CC[CT]
FspI 4253 TGCGCA TGC^GCA
HincII 4448 GTTAAC GT[CT]^[GA]AC
HindII 4448 GTTAAC GT[CT]^[GA]AC
HpaI 4448 GTTAAC GTT^AAC
KspI 4305 CCGCGG CCGC^GG
MfeI 658 CAATTG C^AATTG
MunI 658 CAATTG C^AATTG
NdeI 6274 CATATG CA^TATG
NheI 881 GCTAGC G^CTAGC
NspV 3590 TTCGAA TT^CGAA
PpuMI 1605 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3617 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 6021 ATTAAT AT^TAAT
PvuI 1885 CGATCG CGAT^CG
SacII 4305 CCGCGG CCGC^GG
ScaI 4263 AGTACT AGT^ACT
SfiI 4280 GGCCTGGTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 3590 TTCGAA TT^CGAA
SmaI 1794 CCCGGG CCC^GGG
SphI 4131 GCATGC GCATG^C
XbaI 425 TCTAGA T^CTAGA
XmaCI 1794 CCCGGG C^CCGGG
XmaI 1794 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 4395 TCCGGA T^CCGGA
4770 TCCGGA
Alw44I 1655 GTGCAC G^TGCAC
4097 GTGCAC
AocI 5269 CCTCAGG CC^T[ACGT]AGG
5776 CCTGAGG
ApaLI 1655 GTGCAC G^TGCAC
4097 GTGCAC
AspI 3140 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4736 GACAGGGTC
AvaI 1794 CCCGGG C^[CT]CG[GA]G
4815 CTCGGG
BanI 310 GGCACC G^G[CT][GA]CC
2858 GGTGCC
BlpI 1010 GCTAAGC GC^T[ACGT]AGC
1423 GCTCAGC
Bpu1102I 1010 GCTAAGC GC^T[ACGT]AGC
1423 GCTCAGC
BsaBI 579 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2242 GATGAACATC
BseAI 4395 TCCGGA T^CCGGA
4770 TCCGGA
BsiEI 1885 CGATCG CG[GA][CT]^CG
3259 CGACCG
BsmBI 5198 CGTCTC CGTCTC(1/5)
595 GAGACG
BsmI 2443 GAATGC GAATGC(1/-1)
1198 GCATTC
BsoBI 1794 CCCGGG C^[CT]CG[GA]G
4815 CTCGGG
BspEI 4395 TCCGGA T^CCGGA
4770 TCCGGA
BsrBI 1460 CCGCTC CCGCTC(-3/-3)
5285 GAGCGG
BsrFI 895 GCCGGT [GA]^CCGG[CT]
2855 GCCGGT
BssSI 747 CACGAG CACGAG(-5/-1)
1463 CTCGTG
BstAPI 1428 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1477 GCACCTGGTGC
BstEII 1357 GGTGACC G^GT[ACGT]ACC
2179 GGTCACC
BstPI 1357 GGTGACC G^GT[ACGT]ACC
2179 GGTCACC
Bsu36I 5269 CCTCAGG CC^T[ACGT]AGG
5776 CCTGAGG
CelII 1010 GCTAAGC GC^T[ACGT]AGC
1423 GCTCAGC
Cfr10I 895 GCCGGT [GA]^CCGG[CT]
2855 GCCGGT
Eco81I 5269 CCTCAGG CC^T[ACGT]AGG
5776 CCTGAGG
EcoO65I 1357 GGTGACC G^GT[ACGT]ACC
2179 GGTCACC
EcoT22I 1196 ATGCAT ATGCA^T
6032 ATGCAT
HaeII 1402 GGCGCT [GA]GCGC^[CT]
1706 GGCGCT
HgaI 4899 GCGTC GACGC(5/10)
5197 GCGTC
MamI 579 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2242 GATGAACATC
MroI 4395 TCCGGA T^CCGGA
4770 TCCGGA
NcoI 1518 CCATGG C^CATGG
5549 CCATGG
NsiI 1196 ATGCAT ATGCA^T
6032 ATGCAT
PflFI 3140 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4736 GACAGGGTC
SexAI 132 ACCTGGT A^CC[AT]GGT
1479 ACCTGGT
Tth111I 3140 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4736 GACAGGGTC
XcmI 859 CCAGTTTATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1368 CCATCCGCGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BamHI BanII
BbsI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraIII DsaI EaeI EarI
EcoNI EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI