##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BciVI BglI BglII BlnI
BseAI BsiWI BspEI BssHII Bst1107I
BstBI BstZ17I Bsu36I CpoI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI FseI FspI HindIII HpaI
KpnI KspI MluI MroI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RsrII SacII SalI
ScaI SfiI SfuI SphI SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2868 GACGTC GACGT^C
AccII 4126 CGCG CG^CG
AflII 2312 CTTAAG C^TTAAG
AflIII 4768 ACATGT A^C[GA][CT]GT
BamHI 1420 GGATCC G^GATCC
BbeI 3010 GGCGCC GGCGC^C
BbrPI 5317 CACGTG CAC^GTG
BbvCI 5228 GCTGAGG CCTCAGC(-5/-2)
BfrI 2312 CTTAAG C^TTAAG
BsaBI 479 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4651 ACCGGA [AT]^CCGG[AT]
BsiEI 2606 CGATCG CG[GA][CT]^CG
BsmBI 3003 GAGACG CGTCTC(1/5)
BspDI 2580 ATCGAT AT^CGAT
BspHI 3164 TCATGA T^CATGA
BspLU11I 4768 ACATGT A^CATGT
BstEII 2535 GGTGACC G^GT[ACGT]ACC
BstPI 2535 GGTGACC G^GT[ACGT]ACC
BstUI 4126 CGCG CG^CG
ClaI 2580 ATCGAT AT^CGAT
EcoO65I 2535 GGTGACC G^GT[ACGT]ACC
EcoT22I 1146 ATGCAT ATGCA^T
KasI 3010 GGCGCC G^GCGCC
MamI 479 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1543 CAATTG C^AATTG
MunI 1543 CAATTG C^AATTG
MvnI 4126 CGCG CG^CG
NarI 3010 GGCGCC GG^CGCC
NcoI 2977 CCATGG C^CATGG
NheI 2181 GCTAGC G^CTAGC
NsiI 1146 ATGCAT ATGCA^T
PmaCI 5317 CACGTG CAC^GTG
PmlI 5317 CACGTG CAC^GTG
PshAI 3827 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2606 CGATCG CGAT^CG
RcaI 3164 TCATGA T^CATGA
SbfI 890 CCTGCAGG CCTGCA^GG
SexAI 2712 ACCTGGT A^CC[AT]GGT
SfoI 3010 GGCGCC GGC^GCC
SgrAI 3636 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 2326 TACGTA TAC^GTA
SpeI 5492 ACTAGT A^CTAGT
Sse8387I 890 CCTGCAGG CCTGCA^GG
XbaI 110 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AccI 4504 GTAGAC GT^[AC][GT]AC
4953 GTAGAC
ApaI 1013 GGGCCC GGGCC^C
3914 GGGCCC
BlpI 2796 GCTGAGC GC^T[ACGT]AGC
4517 GCTGAGC
Bpu1102I 2796 GCTGAGC GC^T[ACGT]AGC
4517 GCTGAGC
BsaAI 2326 TACGTA [CT]AC^GT[GA]
5317 CACGTG
BstAPI 221 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
914 GCATGATGTGC
CelII 2796 GCTGAGC GC^T[ACGT]AGC
4517 GCTGAGC
EcoRI 1623 GAATTC G^AATTC
5554 GAATTC
EcoRV 4281 GATATC GAT^ATC
4359 GATATC
SapI 3542 GAAGAGC GCTCTTC(1/4)
5535 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcgI BclI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco47III EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI