##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccI AccIII AclI Aor51HI
AscI Asp700I AviII AvrII BamHI
BcgI BciVI BlnI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HpaI KspI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcyI 3600 GGCGCC G[GA]^CG[CT]C
AflII 5157 CTTAAG C^TTAAG
AflIII 5320 ACATGT A^C[GA][CT]GT
AgeI 894 ACCGGT A^CCGGT
AhdI 3025 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2706 GTGCAC G^TGCAC
ApaLI 2706 GTGCAC G^TGCAC
AspEI 3025 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 3600 GGCGCC GGCGC^C
BbrPI 2462 CACGTG CAC^GTG
BclI 2935 TGATCA T^GATCA
BfrI 5157 CTTAAG C^TTAAG
BglII 3268 AGATCT A^GATCT
BsaAI 2462 CACGTG [CT]AC^GT[GA]
BsaBI 343 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3600 GGCGCC G[GA]^CG[CT]C
BsaWI 894 ACCGGT [AT]^CCGG[AT]
BspLU11I 5320 ACATGT A^CATGT
BsrBI 235 GAGCGG CCGCTC(-3/-3)
BstEII 3047 GGTCACC G^GT[ACGT]ACC
BstPI 3047 GGTCACC G^GT[ACGT]ACC
DraIII 1484 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3025 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3047 GGTCACC G^GT[ACGT]ACC
EcoRI 4019 GAATTC G^AATTC
FbaI 2935 TGATCA T^GATCA
Hin1I 3600 GGCGCC G[GA]^CG[CT]C
HincII 169 GTCAAC GT[CT]^[GA]AC
HindII 169 GTCAAC GT[CT]^[GA]AC
HindIII 3207 AAGCTT A^AGCTT
KasI 3600 GGCGCC G^GCGCC
MamI 343 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3600 GGCGCC GG^CGCC
NcoI 4321 CCATGG C^CATGG
NheI 1815 GCTAGC G^CTAGC
PinAI 894 ACCGGT A^CCGGT
PmaCI 2462 CACGTG CAC^GTG
PmlI 2462 CACGTG CAC^GTG
SbfI 297 CCTGCAGG CCTGCA^GG
SexAI 1485 ACCTGGT A^CC[AT]GGT
SfoI 3600 GGCGCC GGC^GCC
Sse8387I 297 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1613 GGTACC G^GTACC
2692 GGTACC
AocI 784 CCTGAGG CC^T[ACGT]AGG
2491 CCTGAGG
AseI 1072 ATTAAT AT^TAAT
1079 ATTAAT
AsnI 1072 ATTAAT AT^TAAT
1079 ATTAAT
Asp718I 1613 GGTACC G^GTACC
2692 GGTACC
AspI 1044 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5083 GACTTCGTC
AvaI 2574 CCCGGG C^[CT]CG[GA]G
2743 CCCGGG
BsiEI 1707 CGACCG CG[GA][CT]^CG
2422 CGGTCG
BsoBI 2574 CCCGGG C^[CT]CG[GA]G
2743 CCCGGG
BssSI 2162 CACGAG CACGAG(-5/-1)
4874 CACGAG
BstAPI 2895 GCAGGCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3398 GCAAGTGGTGC
BstYI 2800 GGATCT [GA]^GATC[CT]
3268 AGATCT
Bsu36I 784 CCTGAGG CC^T[ACGT]AGG
2491 CCTGAGG
Eco81I 784 CCTGAGG CC^T[ACGT]AGG
2491 CCTGAGG
HaeII 3600 GGCGCC [GA]GCGC^[CT]
3813 AGCGCC
KpnI 1613 GGTACC GGTAC^C
2692 GGTACC
MflI 2800 GGATCT [GA]^GATC[CT]
3268 AGATCT
NaeI 2268 GCCGGC GCC^GGC
2479 GCCGGC
NgoMIV 2268 GCCGGC G^CCGGC
2479 GCCGGC
PflFI 1044 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5083 GACTTCGTC
PshBI 1072 ATTAAT AT^TAAT
1079 ATTAAT
SacI 1521 GAGCTC GAGCT^C
1554 GAGCTC
SmaI 2574 CCCGGG CCC^GGG
2743 CCCGGG
SmlI 5157 CTTAAG C^T[CT][GA]AG
5311 CTTGAG
SphI 2427 GCATGC GCATG^C
5144 GCATGC
Tth111I 1044 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
5083 GACTTCGTC
XhoII 2800 GGATCT [GA]^GATC[CT]
3268 AGATCT
XmaCI 2574 CCCGGG C^CCGGG
2743 CCCGGG
XmaI 2574 CCCGGG C^CCGGG
2743 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI