##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspI BcgI
BciVI BfrI BglII BspDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI Eco47III EcoRI HindIII
HpaI KpnI KspI MluI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 491 GACGTC GACGT^C
AccIII 1347 TCCGGA T^CCGGA
AhdI 6 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 6 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1310 TGCGCA TGC^GCA
AvrII 404 CCTAGG C^CTAGG
BamHI 1045 GGATCC G^GATCC
BclI 5119 TGATCA T^GATCA
BlnI 404 CCTAGG C^CTAGG
BlpI 4606 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4606 GCTCAGC GC^T[ACGT]AGC
BsaBI 4534 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1347 TCCGGA [AT]^CCGG[AT]
BseAI 1347 TCCGGA T^CCGGA
BsiEI 4646 CGGCCG CG[GA][CT]^CG
BsiWI 4778 CGTACG C^GTACG
Bsp1407I 4584 TGTACA T^GTACA
BspEI 1347 TCCGGA T^CCGGA
BsrGI 4584 TGTACA T^GTACA
BssSI 4490 CACGAG CACGAG(-5/-1)
CelII 4606 GCTCAGC GC^T[ACGT]AGC
DraIII 3378 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4646 CGGCCG C^GGCCG
Eam1105I 6 GACCCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4646 CGGCCG C^GGCCG
Eco52I 4646 CGGCCG C^GGCCG
EcoT22I 2466 ATGCAT ATGCA^T
FbaI 5119 TGATCA T^GATCA
FspI 1310 TGCGCA TGC^GCA
HincII 4739 GTCAAC GT[CT]^[GA]AC
HindII 4739 GTCAAC GT[CT]^[GA]AC
MamI 4534 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4270 CAATTG C^AATTG
MroI 1347 TCCGGA T^CCGGA
MunI 4270 CAATTG C^AATTG
NsiI 2466 ATGCAT ATGCA^T
PshAI 4717 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3144 GAGCTC GAGCT^C
SapI 4999 GCTCTTC GCTCTTC(1/4)
SfiI 412 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4584 TGTACA T^GTACA
XcmI 3047 CCAGTGGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbeI 1182 GGCGCC GGCGC^C
5283 GGCGCC
BsaI 4 GAGACC GGTCTC(1/5)
2235 GGTCTC
BspLU11I 485 ACATGT A^CATGT
2720 ACATGT
BsrBI 3771 CCGCTC CCGCTC(-3/-3)
4378 CCGCTC
BsrDI 789 GCAATG GCAATG(2/0)
1564 GCAATG
BstAPI 1891 GCATTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4446 GCATCGGCTGC
EcoRV 898 GATATC GAT^ATC
5429 GATATC
FseI 1720 GGCCGGCC GGCCGG^CC
2899 GGCCGGCC
KasI 1182 GGCGCC G^GCGCC
5283 GGCGCC
NarI 1182 GGCGCC GG^CGCC
5283 GGCGCC
PaeR7I 3391 CTCGAG C^TCGAG
4734 CTCGAG
PstI 127 CTGCAG CTGCA^G
3629 CTGCAG
SfoI 1182 GGCGCC GGC^GCC
5283 GGCGCC
SmaI 1136 CCCGGG CCC^GGG
2401 CCCGGG
SphI 3742 GCATGC GCATG^C
5171 GCATGC
XhoI 3391 CTCGAG C^TCGAG
4734 CTCGAG
XmaCI 1136 CCCGGG C^CCGGG
2401 CCCGGG
XmaI 1136 CCCGGG C^CCGGG
2401 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII