##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI AviII BamHI BbeI
BbrPI BbvCI BcgI BfrI BglI
BglII BlpI Bpu1102I BsaAI BsaHI
BseAI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstZ17I CelII CfoI Cfr10I CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoT22I FseI FspI HaeII HhaI
Hin1I HinP1I KasI KspI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3094 GGTACC G^GTACC
AccI 1843 GTCTAC GT^[AC][GT]AC
ApaI 1629 GGGCCC GGGCC^C
Asp718I 3094 GGTACC G^GTACC
AspI 170 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2445 CCTAGG C^CTAGG
BalI 3806 TGGCCA TGG^CCA
BanI 3094 GGTACC G^G[CT][GA]CC
BlnI 2445 CCTAGG C^CTAGG
BsaWI 4180 ACCGGA [AT]^CCGG[AT]
BsmI 4255 GAATGC GAATGC(1/-1)
Bsp1407I 5405 TGTACA T^GTACA
BspDI 4072 ATCGAT AT^CGAT
BsrGI 5405 TGTACA T^GTACA
BstBI 2254 TTCGAA TT^CGAA
BstEII 1275 GGTCACC G^GT[ACGT]ACC
BstPI 1275 GGTCACC G^GT[ACGT]ACC
ClaI 4072 ATCGAT AT^CGAT
DrdI 4191 GACCGACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1275 GGTCACC G^GT[ACGT]ACC
EcoRV 2914 GATATC GAT^ATC
HgaI 4865 GCGTC GACGC(5/10)
HincII 815 GTTAAC GT[CT]^[GA]AC
HindII 815 GTTAAC GT[CT]^[GA]AC
HpaI 815 GTTAAC GTT^AAC
KpnI 3094 GGTACC GGTAC^C
MfeI 3451 CAATTG C^AATTG
MluNI 3806 TGGCCA TGG^CCA
MscI 3806 TGGCCA TGG^CCA
MunI 3451 CAATTG C^AATTG
NspV 2254 TTCGAA TT^CGAA
PaeR7I 2320 CTCGAG C^TCGAG
PflFI 170 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
SexAI 3443 ACCAGGT A^CC[AT]GGT
SfuI 2254 TTCGAA TT^CGAA
SmaI 1318 CCCGGG CCC^GGG
SspBI 5405 TGTACA T^GTACA
SspI 4791 AATATT AAT^ATT
Tth111I 170 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4868 TCTAGA T^CTAGA
XhoI 2320 CTCGAG C^TCGAG
XmaCI 1318 CCCGGG C^CCGGG
XmaI 1318 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 2001 CGCG CG^CG
5169 CGCG
AflIII 418 ACATGT A^C[GA][CT]GT
4424 ACATGT
AhdI 3312 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4179 GACCGGAAGTC
AlwNI 3670 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
4537 CAGATTCTG
AocI 3351 CCTCAGG CC^T[ACGT]AGG
4245 CCTGAGG
AspEI 3312 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4179 GACCGGAAGTC
BclI 1028 TGATCA T^GATCA
4832 TGATCA
BsaBI 2745 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2910 GATAGATATC
BspHI 2645 TCATGA T^CATGA
5512 TCATGA
BspLU11I 418 ACATGT A^CATGT
4424 ACATGT
BspMI 1736 ACCTGC ACCTGC(4/8)
4469 ACCTGC
BssSI 4303 CACGAG CACGAG(-5/-1)
5146 CTCGTG
BstUI 2001 CGCG CG^CG
5169 CGCG
Bsu36I 3351 CCTCAGG CC^T[ACGT]AGG
4245 CCTGAGG
Eam1105I 3312 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4179 GACCGGAAGTC
Eco81I 3351 CCTCAGG CC^T[ACGT]AGG
4245 CCTGAGG
EcoNI 765 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1452 CCTCTTTCAGG
EcoRI 974 GAATTC G^AATTC
3653 GAATTC
FauI 3029 GCGGG CCCGC(4/6)
5361 GCGGG
FbaI 1028 TGATCA T^GATCA
4832 TGATCA
HindIII 3182 AAGCTT A^AGCTT
4806 AAGCTT
MamI 2745 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2910 GATAGATATC
MvnI 2001 CGCG CG^CG
5169 CGCG
NdeI 342 CATATG CA^TATG
3135 CATATG
RcaI 2645 TCATGA T^CATGA
5512 TCATGA
SapI 2290 GAAGAGC GCTCTTC(1/4)
2469 GAAGAGC
SmlI 2320 CTCGAG C^T[CT][GA]AG
5057 CTTGAG
SphI 556 GCATGC GCATG^C
3914 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaI AvaII
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI