##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI AspEI AviII
BbrPI BbsI BclI BfrI BglII
BpuAI BsaAI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2788 GGTACC G^GTACC
Aor51HI 1180 AGCGCT AGC^GCT
Asp718I 2788 GGTACC G^GTACC
AvrII 4616 CCTAGG C^CTAGG
BbeI 4657 GGCGCC GGCGC^C
BciVI 3085 GTATCC GTATCC(6/5)
BlnI 4616 CCTAGG C^CTAGG
BsaBI 3701 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1022 CGGCCG CG[GA][CT]^CG
BsmI 3220 GAATGC GAATGC(1/-1)
BsrDI 248 CATTGC GCAATG(2/0)
BstEII 575 GGTGACC G^GT[ACGT]ACC
BstPI 575 GGTGACC G^GT[ACGT]ACC
BstXI 2341 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 3937 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1796 GACACAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1022 CGGCCG C^GGCCG
EclXI 1022 CGGCCG C^GGCCG
Eco47III 1180 AGCGCT AGC^GCT
Eco52I 1022 CGGCCG C^GGCCG
EcoO65I 575 GGTGACC G^GT[ACGT]ACC
FseI 1019 GGCCGGCC GGCCGG^CC
KasI 4657 GGCGCC G^GCGCC
KpnI 2788 GGTACC GGTAC^C
MaeII 4774 ACGT A^CGT
MamI 3701 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 4845 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 4657 GGCGCC GG^CGCC
NspI 3576 ACATGC [GA]CATG^[CT]
SexAI 4681 ACCTGGT A^CC[AT]GGT
SfoI 4657 GGCGCC GGC^GCC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1882 AAATTC [GA]^AATT[CT]
2754 GAATTT
ApoI 1882 AAATTC [GA]^AATT[CT]
2754 GAATTT
Asp700I 194 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
453 GAAGGCGTTC
AspI 677 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4467 GACAGGGTC
BamHI 1347 GGATCC G^GATCC
5081 GGATCC
BlpI 837 GCTCAGC GC^T[ACGT]AGC
2817 GCTGAGC
Bpu1102I 837 GCTCAGC GC^T[ACGT]AGC
2817 GCTGAGC
BsaWI 3402 ACCGGA [AT]^CCGG[AT]
4765 ACCGGA
BstAPI 1499 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3475 GCAGGCCCTGC
CelII 837 GCTCAGC GC^T[ACGT]AGC
2817 GCTGAGC
KspI 2920 CCGCGG CCGC^GG
3463 CCGCGG
MseI 4214 TTAA T^TAA
5195 TTAA
NcoI 1741 CCATGG C^CATGG
4040 CCATGG
PflFI 677 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4467 GACAGGGTC
SacII 2920 CCGCGG CCGC^GG
3463 CCGCGG
SapI 171 GAAGAGC GCTCTTC(1/4)
1155 GAAGAGC
SfiI 968 GGCCAAGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3634 GGCCCAGCGGGCC
SmaI 38 CCCGGG CCC^GGG
3121 CCCGGG
Tru9I 4214 TTAA T^TAA
5195 TTAA
Tsp509I 1883 AATT ^AATT
2755 AATT
Tth111I 677 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4467 GACAGGGTC
XmaCI 38 CCCGGG C^CCGGG
3121 CCCGGG
XmaI 38 CCCGGG C^CCGGG
3121 CCCGGG
XmnI 194 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
453 GAAGGCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoI XhoII