##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII BamHI BsiWI
BspDI BspLU11I BsrBI BssSI BstBI
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRV FseI
FspI KpnI KspI MfeI MunI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmeI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 3336 TCCGGA T^CCGGA
BcgI 2291 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2822 GGATAC GTATCC(6/5)
BglII 1050 AGATCT A^GATCT
BsaBI 4567 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3336 TCCGGA [AT]^CCGG[AT]
BseAI 3336 TCCGGA T^CCGGA
BsiEI 4279 CGGTCG CG[GA][CT]^CG
BspEI 3336 TCCGGA T^CCGGA
BsrFI 1466 GCCGGT [GA]^CCGG[CT]
BssHII 2334 GCGCGC G^CGCGC
Bst1107I 1447 GTATAC GTA^TAC
BstZ17I 1447 GTATAC GTA^TAC
Cfr10I 1466 GCCGGT [GA]^CCGG[CT]
DrdI 706 GACAGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 136 CCTCCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 894 GAATTC G^AATTC
EcoT22I 2074 ATGCAT ATGCA^T
HindIII 925 AAGCTT A^AGCTT
HpaI 1983 GTTAAC GTT^AAC
MamI 4567 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 285 ACGCGT A^CGCGT
MroI 3336 TCCGGA T^CCGGA
NheI 5082 GCTAGC G^CTAGC
NsiI 2074 ATGCAT ATGCA^T
PaeR7I 3576 CTCGAG C^TCGAG
SapI 187 GCTCTTC GCTCTTC(1/4)
SphI 4216 GCATGC GCATG^C
SspI 2994 AATATT AAT^ATT
StuI 1194 AGGCCT AGG^CCT
XbaI 3080 TCTAGA T^CTAGA
XhoI 3576 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1447 GTATAC GT^[AC][GT]AC
3886 GTCTAC
AflII 613 CTTAAG C^TTAAG
2934 CTTAAG
AflIII 285 ACGCGT A^C[GA][CT]GT
365 ACGTGT
ApaI 782 GGGCCC GGGCC^C
2646 GGGCCC
Asp700I 2189 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2905 GAATTTTTTC
BalI 2393 TGGCCA TGG^CCA
3563 TGGCCA
BbeI 58 GGCGCC GGCGC^C
3417 GGCGCC
BbrPI 1717 CACGTG CAC^GTG
4961 CACGTG
BclI 2615 TGATCA T^GATCA
4111 TGATCA
BfrI 613 CTTAAG C^TTAAG
2934 CTTAAG
BglI 3420 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3556 GCCATGTTGGC
BlpI 453 GCTCAGC GC^T[ACGT]AGC
1232 GCTCAGC
Bpu1102I 453 GCTCAGC GC^T[ACGT]AGC
1232 GCTCAGC
BsaAI 1717 CACGTG [CT]AC^GT[GA]
4961 CACGTG
BsmI 1291 GAATGC GAATGC(1/-1)
4665 GAATGC
Bsp1407I 4488 TGTACA T^GTACA
5268 TGTACA
BspHI 1368 TCATGA T^CATGA
4834 TCATGA
BsrGI 4488 TGTACA T^GTACA
5268 TGTACA
BstAPI 1861 GCAATCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4814 GCAACTGCTGC
BstEII 343 GGTGACC G^GT[ACGT]ACC
3175 GGTGACC
BstPI 343 GGTGACC G^GT[ACGT]ACC
3175 GGTGACC
CelII 453 GCTCAGC GC^T[ACGT]AGC
1232 GCTCAGC
EcoO65I 343 GGTGACC G^GT[ACGT]ACC
3175 GGTGACC
FbaI 2615 TGATCA T^GATCA
4111 TGATCA
HgaI 287 GCGTC GACGC(5/10)
2213 GCGTC
KasI 58 GGCGCC G^GCGCC
3417 GGCGCC
MluNI 2393 TGGCCA TGG^CCA
3563 TGGCCA
MscI 2393 TGGCCA TGG^CCA
3563 TGGCCA
NarI 58 GGCGCC GG^CGCC
3417 GGCGCC
NcoI 72 CCATGG C^CATGG
3328 CCATGG
NspI 3882 GCATGT [GA]CATG^[CT]
4216 GCATGC
PflMI 905 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1238 CCAGGCGCTGG
PmaCI 1717 CACGTG CAC^GTG
4961 CACGTG
PmlI 1717 CACGTG CAC^GTG
4961 CACGTG
PvuII 45 CAGCTG CAG^CTG
938 CAGCTG
RcaI 1368 TCATGA T^CATGA
4834 TCATGA
SfoI 58 GGCGCC GGC^GCC
3417 GGCGCC
SspBI 4488 TGTACA T^GTACA
5268 TGTACA
Van91I 905 CCACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1238 CCAGGCGCTGG
XcmI 1772 CCACACCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1969 CCAGTTCTCTAGTGG
XmnI 2189 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2905 GAATTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaI AvaII
AvrII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PshBI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI