##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI BamHI BbeI BcgI
BfrI BglII BsiWI BspDI BspLU11I
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EagI
EclXI Eco52I EcoO65I EcoT22I FseI
HindIII HpaI KasI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I RsrII SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 4697 GTCTAC GT^[AC][GT]AC
AcsI 2478 GAATTC [GA]^AATT[CT]
ApoI 2478 GAATTC [GA]^AATT[CT]
AspI 2670 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5007 TGCGCA TGC^GCA
AvrII 872 CCTAGG C^CTAGG
BciVI 1394 GGATAC GTATCC(6/5)
BlnI 872 CCTAGG C^CTAGG
BsaBI 1395 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2087 CGTCTC CGTCTC(1/5)
BspHI 2853 TCATGA T^CATGA
BsrDI 264 CATTGC GCAATG(2/0)
BssHII 3373 GCGCGC G^CGCGC
BssSI 749 CTCGTG CACGAG(-5/-1)
EcoRI 2478 GAATTC G^AATTC
EcoRV 3244 GATATC GAT^ATC
FspI 5007 TGCGCA TGC^GCA
KspI 4986 CCGCGG CCGC^GG
MamI 1395 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3987 CAATTG C^AATTG
MluI 4885 ACGCGT A^CGCGT
MunI 3987 CAATTG C^AATTG
PflFI 2670 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3736 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4567 CGATCG CGAT^CG
RcaI 2853 TCATGA T^CATGA
SacII 4986 CCGCGG CCGC^GG
SfiI 4447 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 4174 GCATGC GCATG^C
Tth111I 2670 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1748 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3864 GGTACC G^GTACC
4788 GGTACC
AccIII 3553 TCCGGA T^CCGGA
4617 TCCGGA
AflIII 638 ACGTGT A^C[GA][CT]GT
4885 ACGCGT
AhdI 3211 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4991 GACCCTCTGTC
Alw44I 3382 GTGCAC G^TGCAC
3974 GTGCAC
ApaLI 3382 GTGCAC G^TGCAC
3974 GTGCAC
Asp700I 2177 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2478 GAATTCCTTC
Asp718I 3864 GGTACC G^GTACC
4788 GGTACC
AspEI 3211 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4991 GACCCTCTGTC
BalI 1126 TGGCCA TGG^CCA
4473 TGGCCA
BclI 1400 TGATCA T^GATCA
1507 TGATCA
BseAI 3553 TCCGGA T^CCGGA
4617 TCCGGA
BsiEI 2563 CGACCG CG[GA][CT]^CG
4567 CGATCG
BsmI 3701 GAATGC GAATGC(1/-1)
2881 GCATTC
Bsp1407I 2797 TGTACA T^GTACA
3134 TGTACA
BspEI 3553 TCCGGA T^CCGGA
4617 TCCGGA
BsrGI 2797 TGTACA T^GTACA
3134 TGTACA
Eam1105I 3211 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4991 GACCCTCTGTC
FbaI 1400 TGATCA T^GATCA
1507 TGATCA
HincII 3896 GTCAAC GT[CT]^[GA]AC
4340 GTCAAC
HindII 3896 GTCAAC GT[CT]^[GA]AC
4340 GTCAAC
KpnI 3864 GGTACC GGTAC^C
4788 GGTACC
MluNI 1126 TGGCCA TGG^CCA
4473 TGGCCA
MroI 3553 TCCGGA T^CCGGA
4617 TCCGGA
MscI 1126 TGGCCA TGG^CCA
4473 TGGCCA
NaeI 2604 GCCGGC GCC^GGC
2647 GCCGGC
NgoMIV 2604 GCCGGC G^CCGGC
2647 GCCGGC
NspI 715 GCATGT [GA]CATG^[CT]
4174 GCATGC
SexAI 3098 ACCAGGT A^CC[AT]GGT
4425 ACCTGGT
SmlI 3388 CTCAAG C^T[CT][GA]AG
3430 CTCAAG
SspBI 2797 TGTACA T^GTACA
3134 TGTACA
XmnI 2177 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2478 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI