##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI Asp718I AspEI AviII AvrII
BciVI BglII BlnI BsiWI Bsp1407I
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DrdI Eam1105I
EcoRV FseI FspI KpnI KspI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 1245 CGCG CG^CG
AccIII 3444 TCCGGA T^CCGGA
AflII 3496 CTTAAG C^TTAAG
AflIII 1076 ACGTGT A^C[GA][CT]GT
Aor51HI 3554 AGCGCT AGC^GCT
BamHI 4974 GGATCC G^GATCC
BclI 3275 TGATCA T^GATCA
BfrI 3496 CTTAAG C^TTAAG
BglI 1856 GCCATATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3444 TCCGGA [AT]^CCGG[AT]
BseAI 3444 TCCGGA T^CCGGA
BsiEI 1952 CGGCCG CG[GA][CT]^CG
BsmBI 2867 CGTCTC CGTCTC(1/5)
BspDI 4859 ATCGAT AT^CGAT
BspEI 3444 TCCGGA T^CCGGA
BsrBI 613 GAGCGG CCGCTC(-3/-3)
BsrFI 1640 GCCGGT [GA]^CCGG[CT]
BstEII 872 GGTAACC G^GT[ACGT]ACC
BstPI 872 GGTAACC G^GT[ACGT]ACC
BstUI 1245 CGCG CG^CG
BstYI 4974 GGATCC [GA]^GATC[CT]
Cfr10I 1640 GCCGGT [GA]^CCGG[CT]
ClaI 4859 ATCGAT AT^CGAT
DraI 3097 TTTAAA TTT^AAA
DraIII 4780 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1952 CGGCCG C^GGCCG
EclXI 1952 CGGCCG C^GGCCG
Eco47III 3554 AGCGCT AGC^GCT
Eco52I 1952 CGGCCG C^GGCCG
EcoO65I 872 GGTAACC G^GT[ACGT]ACC
EcoRI 4631 GAATTC G^AATTC
EcoT22I 2169 ATGCAT ATGCA^T
FbaI 3275 TGATCA T^GATCA
HindIII 3586 AAGCTT A^AGCTT
HpaI 4605 GTTAAC GTT^AAC
MflI 4974 GGATCC [GA]^GATC[CT]
MroI 3444 TCCGGA T^CCGGA
MvnI 1245 CGCG CG^CG
NsiI 2169 ATGCAT ATGCA^T
SacI 3239 GAGCTC GAGCT^C
SpeI 4956 ACTAGT A^CTAGT
SspI 4172 AATATT AAT^ATT
XhoII 4974 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 4327 GTCTAC GT^[AC][GT]AC
4462 GTAGAC
AcyI 3975 GGCGCC G[GA]^CG[CT]C
4281 GGCGCC
Alw44I 1279 GTGCAC G^TGCAC
1797 GTGCAC
ApaLI 1279 GTGCAC G^TGCAC
1797 GTGCAC
AspI 168 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5102 GACTCTGTC
BbeI 3975 GGCGCC GGCGC^C
4281 GGCGCC
BbrPI 1075 CACGTG CAC^GTG
1583 CACGTG
BlpI 73 GCTCAGC GC^T[ACGT]AGC
282 GCTCAGC
Bpu1102I 73 GCTCAGC GC^T[ACGT]AGC
282 GCTCAGC
BsaAI 1075 CACGTG [CT]AC^GT[GA]
1583 CACGTG
BsaHI 3975 GGCGCC G[GA]^CG[CT]C
4281 GGCGCC
BsrDI 4376 CATTGC GCAATG(2/0)
194 GCAATG
BssSI 2051 CACGAG CACGAG(-5/-1)
2343 CTCGTG
BstAPI 897 GCAGAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5043 GCAGAGGCTGC
CelII 73 GCTCAGC GC^T[ACGT]AGC
282 GCTCAGC
EcoNI 1150 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2762 CCTGGAGCAGG
HgaI 1318 GACGC GACGC(5/10)
2866 GCGTC
Hin1I 3975 GGCGCC G[GA]^CG[CT]C
4281 GGCGCC
KasI 3975 GGCGCC G^GCGCC
4281 GGCGCC
NarI 3975 GGCGCC GG^CGCC
4281 GGCGCC
NcoI 4523 CCATGG C^CATGG
5034 CCATGG
PflFI 168 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5102 GACTCTGTC
PmaCI 1075 CACGTG CAC^GTG
1583 CACGTG
PmlI 1075 CACGTG CAC^GTG
1583 CACGTG
SexAI 2829 ACCTGGT A^CC[AT]GGT
4185 ACCTGGT
SfoI 3975 GGCGCC GGC^GCC
4281 GGCGCC
Tth111I 168 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5102 GACTCTGTC
XbaI 2061 TCTAGA T^CTAGA
4884 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmaCI XmaI XmnI