##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AccIII AflII AgeI AhdI
AscI AseI AsnI AspEI AviII
BcgI BfrI BglII BseAI BsiWI
BspEI BspHI BsrBI BssHII Bst1107I
BstEII BstPI BstZ17I CpoI DraIII
DrdI Eam1105I EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HindIII
HpaI MfeI MluI MroI MunI
NcoI NheI NotI NruI PacI
PaeR7I PinAI PmeI PshBI RcaI
RsrII SacI SalI SapI SbfI
SexAI SgrAI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 904 GACGTC GACGT^C
Acc65I 327 GGTACC G^GTACC
AclI 835 AACGTT AA^CGTT
Aor51HI 781 AGCGCT AGC^GCT
Asp700I 5036 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 327 GGTACC G^GTACC
BamHI 876 GGATCC G^GATCC
BbsI 529 GAAGAC GAAGAC(2/6)
BclI 3883 TGATCA T^GATCA
BpuAI 529 GAAGAC GAAGAC(2/6)
BsaBI 118 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2872 ACCGGA [AT]^CCGG[AT]
BspDI 3547 ATCGAT AT^CGAT
BsrFI 3756 GCCGGC [GA]^CCGG[CT]
BssSI 2296 CTCGTG CACGAG(-5/-1)
BstBI 3492 TTCGAA TT^CGAA
Cfr10I 3756 GCCGGC [GA]^CCGG[CT]
ClaI 3547 ATCGAT AT^CGAT
DraI 3985 TTTAAA TTT^AAA
Eco47III 781 AGCGCT AGC^GCT
EcoNI 5126 CCTTTGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3883 TGATCA T^GATCA
KpnI 327 GGTACC GGTAC^C
MamI 118 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3756 GCCGGC GCC^GGC
NdeI 127 CATATG CA^TATG
NgoMIV 3756 GCCGGC G^CCGGC
NspV 3492 TTCGAA TT^CGAA
PflMI 4726 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2443 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 835 AACGTT AA^CGTT
PvuI 516 CGATCG CGAT^CG
ScaI 5081 AGTACT AGT^ACT
SfuI 3492 TTCGAA TT^CGAA
SpeI 4589 ACTAGT A^CTAGT
SphI 4152 GCATGC GCATG^C
StuI 2948 AGGCCT AGG^CCT
Van91I 4726 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2407 TCTAGA T^CTAGA
XmnI 5036 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 486 GTGCAC G^TGCAC
895 GTGCAC
ApaLI 486 GTGCAC G^TGCAC
895 GTGCAC
BbrPI 1304 CACGTG CAC^GTG
5461 CACGTG
BsgI 30 GTGCAG GTGCAG(16/14)
1827 GTGCAG
Bsp1407I 449 TGTACA T^GTACA
3312 TGTACA
BspLU11I 565 ACATGT A^CATGT
5252 ACATGT
BsrGI 449 TGTACA T^GTACA
3312 TGTACA
BstYI 876 GGATCC [GA]^GATC[CT]
2595 AGATCC
EagI 1138 CGGCCG C^GGCCG
5310 CGGCCG
EclXI 1138 CGGCCG C^GGCCG
5310 CGGCCG
Eco52I 1138 CGGCCG C^GGCCG
5310 CGGCCG
MflI 876 GGATCC [GA]^GATC[CT]
2595 AGATCC
PmaCI 1304 CACGTG CAC^GTG
5461 CACGTG
PmlI 1304 CACGTG CAC^GTG
5461 CACGTG
SspBI 449 TGTACA T^GTACA
3312 TGTACA
SspI 3650 AATATT AAT^ATT
5092 AATATT
XcmI 1400 CCAGCGGGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2842 CCAGCTGTGTCGTGG
XhoII 876 GGATCC [GA]^GATC[CT]
2595 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbeI BbvCI BbvI BciVI BcnI
BfaI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XmaCI XmaI