##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccI AflII AscI AseI AsnI
Asp700I AviII BbeI BciVI BfrI
BlpI Bpu1102I BsaBI BsiWI BspDI
BsrDI Bst1107I BstZ17I CelII ClaI
CpoI EcoT22I FspI HpaI KasI
KspI MamI MfeI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PmeI PshAI PshBI
PvuI RsrII SacII SalI SbfI
ScaI SfoI SmaI SnaBI SpeI
SphI Sse8387I SwaI XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 4267 GGTACC G^GTACC
AccIII 3143 TCCGGA T^CCGGA
AclI 2017 AACGTT AA^CGTT
AgeI 1476 ACCGGT A^CCGGT
AhdI 3694 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 355 AGCGCT AGC^GCT
Asp718I 4267 GGTACC G^GTACC
AspEI 3694 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 34 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4333 CCTAGG C^CTAGG
BbrPI 3663 CACGTG CAC^GTG
BbvCI 3413 GCTGAGG CCTCAGC(-5/-2)
BclI 4221 TGATCA T^GATCA
BglII 646 AGATCT A^GATCT
BlnI 4333 CCTAGG C^CTAGG
BsaAI 3663 CACGTG [CT]AC^GT[GA]
BsaI 1103 GAGACC GGTCTC(1/5)
BseAI 3143 TCCGGA T^CCGGA
BsiEI 833 CGGCCG CG[GA][CT]^CG
BspEI 3143 TCCGGA T^CCGGA
BspHI 1709 TCATGA T^CATGA
BssHII 1410 GCGCGC G^CGCGC
BssSI 605 CTCGTG CACGAG(-5/-1)
BstAPI 3316 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1088 TTCGAA TT^CGAA
BstEII 911 GGTGACC G^GT[ACGT]ACC
BstPI 911 GGTGACC G^GT[ACGT]ACC
EagI 833 CGGCCG C^GGCCG
Eam1105I 3694 GACATACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 833 CGGCCG C^GGCCG
Eco47III 355 AGCGCT AGC^GCT
Eco52I 833 CGGCCG C^GGCCG
EcoO65I 911 GGTGACC G^GT[ACGT]ACC
EcoRV 306 GATATC GAT^ATC
FbaI 4221 TGATCA T^GATCA
FseI 834 GGCCGGCC GGCCGG^CC
HincII 3304 GTTGAC GT[CT]^[GA]AC
HindII 3304 GTTGAC GT[CT]^[GA]AC
KpnI 4267 GGTACC GGTAC^C
MluI 2747 ACGCGT A^CGCGT
MroI 3143 TCCGGA T^CCGGA
NspV 1088 TTCGAA TT^CGAA
PflFI 34 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1476 ACCGGT A^CCGGT
PmaCI 3663 CACGTG CAC^GTG
PmlI 3663 CACGTG CAC^GTG
Psp1406I 2017 AACGTT AA^CGTT
RcaI 1709 TCATGA T^CATGA
SapI 2984 GAAGAGC GCTCTTC(1/4)
SexAI 259 ACCTGGT A^CC[AT]GGT
SfiI 3368 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1088 TTCGAA TT^CGAA
SgrAI 1475 CACCGGTG C[GA]^CCGG[CT]G
SspI 4522 AATATT AAT^ATT
Tth111I 34 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1548 GTGCAC G^TGCAC
1567 GTGCAC
AocI 2165 CCTCAGG CC^T[ACGT]AGG
3263 CCTCAGG
ApaI 1503 GGGCCC GGGCC^C
2113 GGGCCC
ApaLI 1548 GTGCAC G^TGCAC
1567 GTGCAC
BamHI 1420 GGATCC G^GATCC
1642 GGATCC
BbsI 749 GAAGAC GAAGAC(2/6)
1311 GTCTTC
BglI 1333 GCCTCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3369 GCCCCCTGGGC
BpuAI 749 GAAGAC GAAGAC(2/6)
1311 GTCTTC
BsaWI 1476 ACCGGT [AT]^CCGG[AT]
3143 TCCGGA
Bsu36I 2165 CCTCAGG CC^T[ACGT]AGG
3263 CCTCAGG
DraIII 376 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1592 CACAGCGTG
DrdI 558 GACAGCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
893 GACGGCAGCGTC
Eco81I 2165 CCTCAGG CC^T[ACGT]AGG
3263 CCTCAGG
EcoNI 4253 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4650 CCTAGAAAAGG
EcoRI 3678 GAATTC G^AATTC
5450 GAATTC
HindIII 635 AAGCTT A^AGCTT
3947 AAGCTT
NaeI 831 GCCGGC GCC^GGC
835 GCCGGC
NcoI 2219 CCATGG C^CATGG
3929 CCATGG
NgoMIV 831 GCCGGC G^CCGGC
835 GCCGGC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvI BcgI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII