##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AhdI Aor51HI AscI
Asp700I AspEI AviII BamHI BglII
BsaBI BsiWI BspDI BspLU11I BstAPI
BstBI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FseI FspI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NspV PacI PmeI PshAI
Psp1406I PvuI RsrII SacII SalI
SfuI SgrAI SnaBI SpeI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 55 GGTACC G^GTACC
AccIII 5211 TCCGGA T^CCGGA
AcsI 2635 AAATTT [GA]^AATT[CT]
AflII 2540 CTTAAG C^TTAAG
AgeI 209 ACCGGT A^CCGGT
ApoI 2635 AAATTT [GA]^AATT[CT]
Asp718I 55 GGTACC G^GTACC
AvrII 4471 CCTAGG C^CTAGG
BbeI 805 GGCGCC GGCGC^C
BcgI 3672 CGATGTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5678 GGATAC GTATCC(6/5)
BclI 791 TGATCA T^GATCA
BfrI 2540 CTTAAG C^TTAAG
BlnI 4471 CCTAGG C^CTAGG
BseAI 5211 TCCGGA T^CCGGA
BsiEI 2986 CGGTCG CG[GA][CT]^CG
Bsp1407I 3099 TGTACA T^GTACA
BspEI 5211 TCCGGA T^CCGGA
BsrGI 3099 TGTACA T^GTACA
BssHII 5294 GCGCGC G^CGCGC
BssSI 5106 CTCGTG CACGAG(-5/-1)
DraI 2628 TTTAAA TTT^AAA
EcoT22I 5423 ATGCAT ATGCA^T
FbaI 791 TGATCA T^GATCA
HindIII 2859 AAGCTT A^AGCTT
HpaI 655 GTTAAC GTT^AAC
KasI 805 GGCGCC G^GCGCC
KpnI 55 GGTACC GGTAC^C
MroI 5211 TCCGGA T^CCGGA
NaeI 5042 GCCGGC GCC^GGC
NarI 805 GGCGCC GG^CGCC
NgoMIV 5042 GCCGGC G^CCGGC
NsiI 5423 ATGCAT ATGCA^T
PaeR7I 5314 CTCGAG C^TCGAG
PinAI 209 ACCGGT A^CCGGT
ScaI 5048 AGTACT AGT^ACT
SfiI 3534 GGCCTAAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 805 GGCGCC GGC^GCC
SphI 1522 GCATGC GCATG^C
SspBI 3099 TGTACA T^GTACA
SspI 2057 AATATT AAT^ATT
XhoI 5314 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 805 GGCGCC G[GA]^CG[CT]C
1086 GGCGTC
AflIII 1632 ACACGT A^C[GA][CT]GT
4942 ACGTGT
AocI 1156 CCTCAGG CC^T[ACGT]AGG
3596 CCTCAGG
AspI 2019 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2141 GACGGAGTC
BlpI 3293 GCTGAGC GC^T[ACGT]AGC
3682 GCTCAGC
Bpu1102I 3293 GCTGAGC GC^T[ACGT]AGC
3682 GCTCAGC
BsaHI 805 GGCGCC G[GA]^CG[CT]C
1086 GGCGTC
BsaWI 209 ACCGGT [AT]^CCGG[AT]
5211 TCCGGA
BspHI 2369 TCATGA T^CATGA
2660 TCATGA
Bst1107I 5153 GTATAC GTA^TAC
5309 GTATAC
BstYI 447 GGATCT [GA]^GATC[CT]
746 GGATCT
BstZ17I 5153 GTATAC GTA^TAC
5309 GTATAC
Bsu36I 1156 CCTCAGG CC^T[ACGT]AGG
3596 CCTCAGG
CelII 3293 GCTGAGC GC^T[ACGT]AGC
3682 GCTCAGC
DraIII 868 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2838 CACAGGGTG
DrdI 251 GACTTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACAGGCAAGTC
Eco81I 1156 CCTCAGG CC^T[ACGT]AGG
3596 CCTCAGG
EcoNI 5442 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5577 CCTCCTCCAGG
HaeII 805 GGCGCC [GA]GCGC^[CT]
3076 AGCGCC
Hin1I 805 GGCGCC G[GA]^CG[CT]C
1086 GGCGTC
MflI 447 GGATCT [GA]^GATC[CT]
746 GGATCT
PflFI 2019 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2141 GACGGAGTC
RcaI 2369 TCATGA T^CATGA
2660 TCATGA
SapI 47 GCTCTTC GCTCTTC(1/4)
5536 GCTCTTC
SexAI 3359 ACCAGGT A^CC[AT]GGT
5344 ACCAGGT
SmaI 279 CCCGGG CCC^GGG
3954 CCCGGG
Tth111I 2019 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2141 GACGGAGTC
XhoII 447 GGATCT [GA]^GATC[CT]
746 GGATCT
XmaCI 279 CCCGGG C^CCGGG
3954 CCCGGG
XmaI 279 CCCGGG C^CCGGG
3954 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AseI AsnI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PshBI
PstI PvuII RsaI SacI Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
SmlI Sse8387I StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI