##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccI AcyI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII BbeI BbrPI
BbvCI BcgI BclI BsaAI BsaHI
BsiWI BsoBI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstZ17I CpoI
Eam1105I Eco47III EcoNI FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 4591 TCCGGA T^CCGGA
AclI 4676 AACGTT AA^CGTT
AflIII 1225 ACACGT A^C[GA][CT]GT
AocI 4391 CCTGAGG CC^T[ACGT]AGG
AspI 4822 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2544 CCTAGG C^CTAGG
BciVI 4194 GGATAC GTATCC(6/5)
BglI 3127 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2544 CCTAGG C^CTAGG
BlpI 225 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 225 GCTCAGC GC^T[ACGT]AGC
BsaBI 3697 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4591 TCCGGA T^CCGGA
BsiEI 44 CGGCCG CG[GA][CT]^CG
BsmBI 2631 CGTCTC CGTCTC(1/5)
BsmI 2932 GAATGC GAATGC(1/-1)
Bsp1407I 2965 TGTACA T^GTACA
BspDI 201 ATCGAT AT^CGAT
BspEI 4591 TCCGGA T^CCGGA
BsrFI 661 ACCGGC [GA]^CCGG[CT]
BsrGI 2965 TGTACA T^GTACA
Bsu36I 4391 CCTGAGG CC^T[ACGT]AGG
CelII 225 GCTCAGC GC^T[ACGT]AGC
CfoI 1351 GCGC GCG^C
Cfr10I 661 ACCGGC [GA]^CCGG[CT]
ClaI 201 ATCGAT AT^CGAT
DrdI 923 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 44 CGGCCG C^GGCCG
EclXI 44 CGGCCG C^GGCCG
Eco52I 44 CGGCCG C^GGCCG
Eco81I 4391 CCTGAGG CC^T[ACGT]AGG
EcoRI 2255 GAATTC G^AATTC
EcoT22I 5459 ATGCAT ATGCA^T
HhaI 1351 GCGC GCG^C
HinP1I 1351 GCGC G^CGC
HindIII 1652 AAGCTT A^AGCTT
MamI 3697 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4591 TCCGGA T^CCGGA
NsiI 5459 ATGCAT ATGCA^T
PflFI 4822 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 4676 AACGTT AA^CGTT
SapI 686 GCTCTTC GCTCTTC(1/4)
SexAI 4791 ACCAGGT A^CC[AT]GGT
SphI 4715 GCATGC GCATG^C
SspBI 2965 TGTACA T^GTACA
StuI 4843 AGGCCT AGG^CCT
Tth111I 4822 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 36 CGCG CG^CG
1350 CGCG
AflII 249 CTTAAG C^TTAAG
5390 CTTAAG
BamHI 1061 GGATCC G^GATCC
1578 GGATCC
BanI 26 GGTGCC G^G[CT][GA]CC
2434 GGTGCC
BbsI 4962 GAAGAC GAAGAC(2/6)
3209 GTCTTC
BcnI 429 CCGGG CC^[GC]GG
1153 CCGGG
BfrI 249 CTTAAG C^TTAAG
5390 CTTAAG
BglII 4975 AGATCT A^GATCT
5126 AGATCT
BpuAI 4962 GAAGAC GAAGAC(2/6)
3209 GTCTTC
BsaWI 289 TCCGGT [AT]^CCGG[AT]
4591 TCCGGA
BsgI 3073 CTGCAC GTGCAG(16/14)
2618 GTGCAG
BspHI 1231 TCATGA T^CATGA
3687 TCATGA
BstUI 36 CGCG CG^CG
1350 CGCG
BstXI 2059 CCATGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4217 CCACCTTCCTGG
DraIII 10 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2979 CACCCTGTG
DsaI 5205 CCATGG C^C[GA][CT]GG
5249 CCATGG
EcoRV 2086 GATATC GAT^ATC
3393 GATATC
FauI 34 CCCGC CCCGC(4/6)
262 GCGGG
MvnI 36 CGCG CG^CG
1350 CGCG
NciI 429 CCGGG CC^[GC]GG
1153 CCGGG
NcoI 5205 CCATGG C^CATGG
5249 CCATGG
PflMI 828 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1733 CCACTCACTGG
RcaI 1231 TCATGA T^CATGA
3687 TCATGA
Van91I 828 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1733 CCACTCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII