##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AvrII BclI
BfrI BlnI BsaBI BsiWI BspDI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI DraI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I RcaI SalI SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 4429 GACGTC GACGT^C
AccIII 960 TCCGGA T^CCGGA
AhdI 935 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 935 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3336 TGCGCA TGC^GCA
BamHI 4319 GGATCC G^GATCC
BciVI 2312 GTATCC GTATCC(6/5)
BglII 2994 AGATCT A^GATCT
BseAI 960 TCCGGA T^CCGGA
Bsp1407I 897 TGTACA T^GTACA
BspEI 960 TCCGGA T^CCGGA
BsrGI 897 TGTACA T^GTACA
BssSI 3989 CACGAG CACGAG(-5/-1)
CpoI 4448 CGGACCG CG^G[AT]CCG
Eam1105I 935 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 3336 TGCGCA TGC^GCA
KspI 3680 CCGCGG CCGC^GG
MroI 960 TCCGGA T^CCGGA
MseI 5123 TTAA T^TAA
NheI 2077 GCTAGC G^CTAGC
PaeR7I 4590 CTCGAG C^TCGAG
PshAI 2928 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3466 CGATCG CGAT^CG
RsrII 4448 CGGACCG CG^G[AT]CCG
SacI 3437 GAGCTC GAGCT^C
SacII 3680 CCGCGG CCGC^GG
SspBI 897 TGTACA T^GTACA
Tru9I 5123 TTAA T^TAA
XhoI 4590 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AocI 2241 CCTCAGG CC^T[ACGT]AGG
4887 CCTGAGG
AspHI 3437 GAGCTC G[AT]GC[AT]^C
4296 GTGCTC
BcgI 1744 CGATCTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4947 GCATGAGCCTCG
BsiHKAI 3437 GAGCTC G[AT]GC[AT]^C
4296 GTGCTC
BsrDI 1535 CATTGC GCAATG(2/0)
4413 GCAATG
BstXI 219 CCAGGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3998 CCACTGACGTGG
BstYI 2994 AGATCT [GA]^GATC[CT]
4319 GGATCC
Bsu36I 2241 CCTCAGG CC^T[ACGT]AGG
4887 CCTGAGG
Eco81I 2241 CCTCAGG CC^T[ACGT]AGG
4887 CCTGAGG
MflI 2994 AGATCT [GA]^GATC[CT]
4319 GGATCC
NaeI 678 GCCGGC GCC^GGC
2717 GCCGGC
NcoI 2117 CCATGG C^CATGG
3375 CCATGG
NgoMIV 678 GCCGGC G^CCGGC
2717 GCCGGC
SexAI 1237 ACCAGGT A^CC[AT]GGT
3028 ACCTGGT
SfiI 499 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4493 GGCCCCGAGGGCC
SphI 463 GCATGC GCATG^C
3339 GCATGC
TfiI 2880 GAATC G^A[AT]TC
4458 GAATC
XbaI 2989 TCTAGA T^CTAGA
3021 TCTAGA
XhoII 2994 AGATCT [GA]^GATC[CT]
4319 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspI AvaI AvaII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XmaCI XmaI XmnI