##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AseI AsnI AspEI AviII BcgI
BsiWI BsmI BspHI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CpoI
Eam1105I EcoRI EcoRV FspI HindIII
KspI MfeI MluI MunI NdeI
NotI NruI NspV PacI PinAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SfuI SgrAI
SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 3214 AAATTC [GA]^AATT[CT]
AflII 3640 CTTAAG C^TTAAG
ApaI 4568 GGGCCC GGGCC^C
ApoI 3214 AAATTC [GA]^AATT[CT]
Asp700I 1210 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4674 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3325 CCTAGG C^CTAGG
BbrPI 3405 CACGTG CAC^GTG
BfrI 3640 CTTAAG C^TTAAG
BlnI 3325 CCTAGG C^CTAGG
BsaBI 1585 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspDI 5414 ATCGAT AT^CGAT
ClaI 5414 ATCGAT AT^CGAT
DraI 4401 TTTAAA TTT^AAA
DraIII 5379 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3482 GACCAAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 2626 GGCCGGCC GGCCGG^CC
HpaI 3837 GTTAAC GTT^AAC
MamI 1585 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 4674 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3405 CACGTG CAC^GTG
PmeI 4400 GTTTAAAC GTTT^AAAC
PmlI 3405 CACGTG CAC^GTG
PshAI 1988 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4562 CCTGCAGG CCTGCA^GG
ScaI 69 AGTACT AGT^ACT
SexAI 1140 ACCTGGT A^CC[AT]GGT
SfiI 2146 GGCCACATAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 4562 CCTGCAGG CCTGCA^GG
Tth111I 4674 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1210 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2239 GGTACC G^GTACC
4888 GGTACC
AccI 1963 GTCTAC GT^[AC][GT]AC
3235 GTAGAC
AflIII 1857 ACATGT A^C[GA][CT]GT
3449 ACATGT
Aor51HI 1419 AGCGCT AGC^GCT
1749 AGCGCT
Asp718I 2239 GGTACC G^GTACC
4888 GGTACC
BclI 2025 TGATCA T^GATCA
4587 TGATCA
BglII 583 AGATCT A^GATCT
972 AGATCT
BsaI 1664 GAGACC GGTCTC(1/5)
5473 GAGACC
BsmBI 1088 GAGACG CGTCTC(1/5)
3622 GAGACG
Bsp1407I 1860 TGTACA T^GTACA
5096 TGTACA
BspLU11I 1857 ACATGT A^CATGT
3449 ACATGT
BsrGI 1860 TGTACA T^GTACA
5096 TGTACA
EagI 1079 CGGCCG C^GGCCG
1554 CGGCCG
EarI 5358 CTCTTC CTCTTC(1/4)
1201 GAAGAG
EclXI 1079 CGGCCG C^GGCCG
1554 CGGCCG
Eco47III 1419 AGCGCT AGC^GCT
1749 AGCGCT
Eco52I 1079 CGGCCG C^GGCCG
1554 CGGCCG
FbaI 2025 TGATCA T^GATCA
4587 TGATCA
HincII 51 GTTGAC GT[CT]^[GA]AC
3837 GTTAAC
HindII 51 GTTGAC GT[CT]^[GA]AC
3837 GTTAAC
KpnI 2239 GGTACC GGTAC^C
4888 GGTACC
Ksp632I 5358 CTCTTC CTCTTC(1/4)
1201 GAAGAG
NheI 244 GCTAGC G^CTAGC
2507 GCTAGC
PaeR7I 239 CTCGAG C^TCGAG
261 CTCGAG
SmaI 1957 CCCGGG CCC^GGG
5081 CCCGGG
SspBI 1860 TGTACA T^GTACA
5096 TGTACA
XhoI 239 CTCGAG C^TCGAG
261 CTCGAG
XmaCI 1957 CCCGGG C^CCGGG
5081 CCCGGG
XmaI 1957 CCCGGG C^CCGGG
5081 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII