##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I ApaLI AspEI
AspI AviII BamHI BbeI BclI
BfrI BsaHI BsaWI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BstEII BstPI ClaI CpoI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HindIII
KasI KspI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfoI
SgrAI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3208 GGTACC G^GTACC
AflIII 1837 ACGTGT A^C[GA][CT]GT
Aor51HI 3442 AGCGCT AGC^GCT
AscI 682 GGCGCGCC GG^CGCGCC
Asp700I 2990 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3208 GGTACC G^GTACC
BbrPI 2119 CACGTG CAC^GTG
BbsI 3349 GTCTTC GAAGAC(2/6)
BcgI 712 GCAGGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3680 GTATCC GTATCC(6/5)
BpuAI 3349 GTCTTC GAAGAC(2/6)
BsiEI 4211 CGGCCG CG[GA][CT]^CG
BsmBI 5065 GAGACG CGTCTC(1/5)
BsmI 2517 GAATGC GAATGC(1/-1)
BsrFI 528 ACCGGC [GA]^CCGG[CT]
BssHII 683 GCGCGC G^CGCGC
Bst1107I 3098 GTATAC GTA^TAC
BstBI 2644 TTCGAA TT^CGAA
BstZ17I 3098 GTATAC GTA^TAC
Cfr10I 528 ACCGGC [GA]^CCGG[CT]
DraIII 1604 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1836 GACGTGTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4211 CGGCCG C^GGCCG
EclXI 4211 CGGCCG C^GGCCG
Eco47III 3442 AGCGCT AGC^GCT
Eco52I 4211 CGGCCG C^GGCCG
HgaI 4163 GCGTC GACGC(5/10)
HincII 4406 GTTAAC GT[CT]^[GA]AC
HindII 4406 GTTAAC GT[CT]^[GA]AC
HpaI 4406 GTTAAC GTT^AAC
KpnI 3208 GGTACC GGTAC^C
NdeI 4456 CATATG CA^TATG
NspV 2644 TTCGAA TT^CGAA
PflMI 2018 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2119 CACGTG CAC^GTG
PmlI 2119 CACGTG CAC^GTG
PpuMI 38 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 2452 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 3844 AGTACT AGT^ACT
SexAI 4419 ACCTGGT A^CC[AT]GGT
SfiI 2915 GGCCAGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2644 TTCGAA TT^CGAA
SmaI 66 CCCGGG CCC^GGG
SnaBI 3334 TACGTA TAC^GTA
Van91I 2018 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 66 CCCGGG C^CCGGG
XmaI 66 CCCGGG C^CCGGG
XmnI 2990 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2625 GTAGAC GT^[AC][GT]AC
3098 GTATAC
AlwNI 2222 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2842 CAGCACCTG
AseI 3881 ATTAAT AT^TAAT
4524 ATTAAT
AsnI 3881 ATTAAT AT^TAAT
4524 ATTAAT
AvaI 66 CCCGGG C^[CT]CG[GA]G
4541 CTCGGG
AvrII 967 CCTAGG C^CTAGG
3478 CCTAGG
BglII 1062 AGATCT A^GATCT
3005 AGATCT
BlnI 967 CCTAGG C^CTAGG
3478 CCTAGG
BsaAI 2119 CACGTG [CT]AC^GT[GA]
3334 TACGTA
BsaBI 1063 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1870 GATCTGGATC
BsoBI 66 CCCGGG C^[CT]CG[GA]G
4541 CTCGGG
BssSI 2174 CACGAG CACGAG(-5/-1)
4023 CTCGTG
BstAPI 2247 GCAAAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3993 GCAACCTCTGC
DraI 3112 TTTAAA TTT^AAA
3867 TTTAAA
EarI 2889 CTCTTC CTCTTC(1/4)
2512 GAAGAG
FauI 163 CCCGC CCCGC(4/6)
788 CCCGC
HaeII 3442 AGCGCT [GA]GCGC^[CT]
5022 GGCGCT
Ksp632I 2889 CTCTTC CTCTTC(1/4)
2512 GAAGAG
MamI 1063 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1870 GATCTGGATC
MfeI 819 CAATTG C^AATTG
2944 CAATTG
MunI 819 CAATTG C^AATTG
2944 CAATTG
PshBI 3881 ATTAAT AT^TAAT
4524 ATTAAT
StuI 1041 AGGCCT AGG^CCT
2269 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApaI ApoI AspHI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII