##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccIII AclI AcyI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AviII AvrII BbeI BcgI BlnI
BsaHI BseAI BseRI BsiEI BspDI
BspEI BspHI BssHII BssSI BstAPI
BstBI BstEII BstPI ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
Hin1I KasI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 827 GGTACC G^GTACC
AccII 4611 CGCG CG^CG
AflII 5310 CTTAAG C^TTAAG
AgeI 4255 ACCGGT A^CCGGT
AhdI 2054 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 629 CCTTAGG CC^T[ACGT]AGG
AseI 1412 ATTAAT AT^TAAT
AsnI 1412 ATTAAT AT^TAAT
Asp718I 827 GGTACC G^GTACC
AspEI 2054 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 481 GTCTTC GAAGAC(2/6)
BfrI 5310 CTTAAG C^TTAAG
BglII 308 AGATCT A^GATCT
BpuAI 481 GTCTTC GAAGAC(2/6)
BsaBI 4013 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4255 ACCGGT [AT]^CCGG[AT]
BsiWI 3708 CGTACG C^GTACG
BsmBI 4901 CGTCTC CGTCTC(1/5)
BsmI 3302 GAATGC GAATGC(1/-1)
Bst1107I 2467 GTATAC GTA^TAC
BstUI 4611 CGCG CG^CG
BstZ17I 2467 GTATAC GTA^TAC
Bsu36I 629 CCTTAGG CC^T[ACGT]AGG
DraII 2880 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 4995 GACTATGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2054 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 629 CCTTAGG CC^T[ACGT]AGG
EcoO109I 2880 AGGCCCC [GA]G^G[ACGT]CC[CT]
HaeII 3231 GGCGCT [GA]GCGC^[CT]
HgaI 433 GACGC GACGC(5/10)
HpaI 684 GTTAAC GTT^AAC
KpnI 827 GGTACC GGTAC^C
MamI 4013 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3411 CAATTG C^AATTG
MunI 3411 CAATTG C^AATTG
MvnI 4611 CGCG CG^CG
PaeR7I 857 CTCGAG C^TCGAG
PinAI 4255 ACCGGT A^CCGGT
PshBI 1412 ATTAAT AT^TAAT
SacI 854 GAGCTC GAGCT^C
ScaI 2569 AGTACT AGT^ACT
SpeI 943 ACTAGT A^CTAGT
XhoI 857 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 2138 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2525 GAATCAGTTC
AvaI 703 CTCGGG C^[CT]CG[GA]G
857 CTCGAG
BamHI 4043 GGATCC G^GATCC
4301 GGATCC
BanI 827 GGTACC G^G[CT][GA]CC
5395 GGCACC
BbrPI 3249 CACGTG CAC^GTG
4772 CACGTG
BbvCI 4555 GCTGAGG CCTCAGC(-5/-2)
4722 GCTGAGG
BciVI 3137 GGATAC GTATCC(6/5)
4756 GTATCC
BcnI 798 CCCGG CC^[GC]GG
4389 CCGGG
BglI 802 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2607 GCCAGTGGGGC
BsaAI 3249 CACGTG [CT]AC^GT[GA]
4772 CACGTG
BsoBI 703 CTCGGG C^[CT]CG[GA]G
857 CTCGAG
Bsp1407I 2209 TGTACA T^GTACA
4137 TGTACA
BspMI 2298 GCAGGT ACCTGC(4/8)
2959 GCAGGT
BsrBI 3227 GAGCGG CCGCTC(-3/-3)
3641 GAGCGG
BsrFI 694 ACCGGC [GA]^CCGG[CT]
4255 ACCGGT
BsrGI 2209 TGTACA T^GTACA
4137 TGTACA
Cfr10I 694 ACCGGC [GA]^CCGG[CT]
4255 ACCGGT
EcoNI 3127 CCTAGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3744 CCTGGATGAGG
HincII 684 GTTAAC GT[CT]^[GA]AC
2837 GTTGAC
HindII 684 GTTAAC GT[CT]^[GA]AC
2837 GTTGAC
HindIII 1167 AAGCTT A^AGCTT
2015 AAGCTT
NciI 798 CCCGG CC^[GC]GG
4389 CCGGG
NcoI 835 CCATGG C^CATGG
2829 CCATGG
PmaCI 3249 CACGTG CAC^GTG
4772 CACGTG
PmlI 3249 CACGTG CAC^GTG
4772 CACGTG
SexAI 3798 ACCTGGT A^CC[AT]GGT
4117 ACCTGGT
SspBI 2209 TGTACA T^GTACA
4137 TGTACA
StuI 1946 AGGCCT AGG^CCT
2622 AGGCCT
XcmI 771 CCAGCTGACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4266 CCATTACCTTAGTGG
XmnI 2138 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2525 GAATCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AspI
AvaII BalI BanII BbvI BclI
BfaI BlpI BmyI BpmI Bpu1102I
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XhoII