##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI BbeI BfrI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI Bst1107I BstBI BstZ17I ClaI
CpoI Eam1105I EcoRI EcoT22I FseI
KasI KpnI MroI NarI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 2446 GACGCC G[GA]^CG[CT]C
Asp700I 1513 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3298 CCCGGG C^[CT]CG[GA]G
AviII 3275 TGCGCA TGC^GCA
BanI 2822 GGCACC G^G[CT][GA]CC
BbrPI 3331 CACGTG CAC^GTG
BcgI 1673 CGAAATATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3096 GTATCC GTATCC(6/5)
BclI 2184 TGATCA T^GATCA
BglII 1059 AGATCT A^GATCT
BsaBI 355 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2446 GACGCC G[GA]^CG[CT]C
BsiEI 3190 CGGCCG CG[GA][CT]^CG
BsoBI 3298 CCCGGG C^[CT]CG[GA]G
BspHI 756 TCATGA T^CATGA
BspLU11I 5349 ACATGT A^CATGT
BsrBI 3778 GAGCGG CCGCTC(-3/-3)
BssHII 92 GCGCGC G^CGCGC
BssSI 2797 CTCGTG CACGAG(-5/-1)
BstAPI 2975 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1371 TTTAAA TTT^AAA
DrdI 3535 GACGAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3190 CGGCCG C^GGCCG
EclXI 3190 CGGCCG C^GGCCG
Eco52I 3190 CGGCCG C^GGCCG
EcoRV 2628 GATATC GAT^ATC
FbaI 2184 TGATCA T^GATCA
FspI 3275 TGCGCA TGC^GCA
Hin1I 2446 GACGCC G[GA]^CG[CT]C
HindIII 941 AAGCTT A^AGCTT
HpaI 472 GTTAAC GTT^AAC
KspI 3193 CCGCGG CCGC^GG
MamI 355 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5250 CAATTG C^AATTG
MluI 3553 ACGCGT A^CGCGT
MunI 5250 CAATTG C^AATTG
NaeI 3415 GCCGGC GCC^GGC
NgoMIV 3415 GCCGGC G^CCGGC
NheI 4244 GCTAGC G^CTAGC
PmaCI 3331 CACGTG CAC^GTG
PmlI 3331 CACGTG CAC^GTG
RcaI 756 TCATGA T^CATGA
SacII 3193 CCGCGG CCGC^GG
SapI 488 GAAGAGC GCTCTTC(1/4)
SbfI 2772 CCTGCAGG CCTGCA^GG
SexAI 1711 ACCTGGT A^CC[AT]GGT
SfiI 3132 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3298 CCCGGG CCC^GGG
Sse8387I 2772 CCTGCAGG CCTGCA^GG
XbaI 2259 TCTAGA T^CTAGA
XmaCI 3298 CCCGGG C^CCGGG
XmaI 3298 CCCGGG C^CCGGG
XmnI 1513 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2339 GTCTAC GT^[AC][GT]AC
3637 GTCTAC
Alw44I 3328 GTGCAC G^TGCAC
4393 GTGCAC
ApaLI 3328 GTGCAC G^TGCAC
4393 GTGCAC
AvrII 2174 CCTAGG C^CTAGG
2948 CCTAGG
BamHI 2218 GGATCC G^GATCC
5228 GGATCC
BbvCI 3663 CCTCAGC CCTCAGC(-5/-2)
2955 GCTGAGG
BlnI 2174 CCTAGG C^CTAGG
2948 CCTAGG
BsaAI 3331 CACGTG [CT]AC^GT[GA]
4616 TACGTG
BsaI 538 GAGACC GGTCTC(1/5)
3394 GAGACC
BsrFI 3415 GCCGGC [GA]^CCGG[CT]
3461 ACCGGC
BstEII 1749 GGTTACC G^GT[ACGT]ACC
2115 GGTCACC
BstPI 1749 GGTTACC G^GT[ACGT]ACC
2115 GGTCACC
BstXI 4918 CCAGCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5471 CCAGCCAGCTGG
Cfr10I 3415 GCCGGC [GA]^CCGG[CT]
3461 ACCGGC
DraIII 3331 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4365 CACTCAGTG
EcoO65I 1749 GGTTACC G^GT[ACGT]ACC
2115 GGTCACC
HincII 472 GTTAAC GT[CT]^[GA]AC
3688 GTCAAC
HindII 472 GTTAAC GT[CT]^[GA]AC
3688 GTCAAC
NcoI 2100 CCATGG C^CATGG
3143 CCATGG
NspI 1144 ACATGC [GA]CATG^[CT]
5349 ACATGT
SacI 2255 GAGCTC GAGCT^C
5459 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI Aor51HI
ApaI ApoI AspHI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII