##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccII AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI BbeI BcgI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BsrFI BssHII BssSI BstUI
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoT22I FseI
HaeII HgaI HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SexAI SfoI SgrAI
SnaBI SpeI SphI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 2842 GACGTC GACGT^C
Acc65I 2578 GGTACC G^GTACC
AccI 4467 GTATAC GT^[AC][GT]AC
AcyI 2842 GACGTC G[GA]^CG[CT]C
AocI 3610 CCTGAGG CC^T[ACGT]AGG
Asp718I 2578 GGTACC G^GTACC
AspI 141 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 556 CCCGGG C^[CT]CG[GA]G
AviII 3341 TGCGCA TGC^GCA
AvrII 3400 CCTAGG C^CTAGG
BamHI 4658 GGATCC G^GATCC
BbrPI 10 CACGTG CAC^GTG
BbsI 3453 GAAGAC GAAGAC(2/6)
BbvCI 3772 CCTCAGC CCTCAGC(-5/-2)
BciVI 823 GGATAC GTATCC(6/5)
BclI 642 TGATCA T^GATCA
BlnI 3400 CCTAGG C^CTAGG
BlpI 3673 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3673 GCTGAGC GC^T[ACGT]AGC
BpuAI 3453 GAAGAC GAAGAC(2/6)
BsaAI 10 CACGTG [CT]AC^GT[GA]
BsaBI 2645 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2842 GACGTC G[GA]^CG[CT]C
BsaWI 542 TCCGGT [AT]^CCGG[AT]
BsmBI 2844 CGTCTC CGTCTC(1/5)
BsoBI 556 CCCGGG C^[CT]CG[GA]G
Bsp1407I 4737 TGTACA T^GTACA
BsrBI 4185 GAGCGG CCGCTC(-3/-3)
BsrDI 504 CATTGC GCAATG(2/0)
BsrGI 4737 TGTACA T^GTACA
Bst1107I 4467 GTATAC GTA^TAC
BstBI 4592 TTCGAA TT^CGAA
BstEII 3495 GGTGACC G^GT[ACGT]ACC
BstPI 3495 GGTGACC G^GT[ACGT]ACC
BstZ17I 4467 GTATAC GTA^TAC
Bsu36I 3610 CCTGAGG CC^T[ACGT]AGG
CelII 3673 GCTGAGC GC^T[ACGT]AGC
Eco81I 3610 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3495 GGTGACC G^GT[ACGT]ACC
EcoRV 1563 GATATC GAT^ATC
FbaI 642 TGATCA T^GATCA
FspI 3341 TGCGCA TGC^GCA
Hin1I 2842 GACGTC G[GA]^CG[CT]C
KpnI 2578 GGTACC GGTAC^C
MamI 2645 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 4324 GCTAGC G^CTAGC
NspV 4592 TTCGAA TT^CGAA
PacI 838 TTAATTAA TTAAT^TAA
PflFI 141 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 10 CACGTG CAC^GTG
PmlI 10 CACGTG CAC^GTG
SapI 215 GAAGAGC GCTCTTC(1/4)
SbfI 4066 CCTGCAGG CCTGCA^GG
ScaI 4889 AGTACT AGT^ACT
SfuI 4592 TTCGAA TT^CGAA
SmaI 556 CCCGGG CCC^GGG
Sse8387I 4066 CCTGCAGG CCTGCA^GG
SspBI 4737 TGTACA T^GTACA
SspI 4351 AATATT AAT^ATT
Tth111I 141 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1981 TCTAGA T^CTAGA
XmaCI 556 CCCGGG C^CCGGG
XmaI 556 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflII 1997 CTTAAG C^TTAAG
2097 CTTAAG
AflIII 2129 ACATGT A^C[GA][CT]GT
2759 ACATGT
AhdI 3456 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5080 GACTGAGAGTC
ApaI 3606 GGGCCC GGGCC^C
3934 GGGCCC
AspEI 3456 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5080 GACTGAGAGTC
BfrI 1997 CTTAAG C^TTAAG
2097 CTTAAG
BspHI 1199 TCATGA T^CATGA
5386 TCATGA
BspLU11I 2129 ACATGT A^CATGT
2759 ACATGT
DraI 2312 TTTAAA TTT^AAA
2356 TTTAAA
DraIII 359 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2141 CACAGAGTG
Eam1105I 3456 GACACGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5080 GACTGAGAGTC
MaeII 11 ACGT A^CGT
2843 ACGT
PflMI 663 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2223 CCAGGAGATGG
RcaI 1199 TCATGA T^CATGA
5386 TCATGA
Van91I 663 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2223 CCAGGAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AspHI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PshAI
PshBI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI