##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AclI AhdI AocI AscI AseI
AsnI Asp700I AspEI AviII BclI
BglII BsiWI BspDI BssHII BstBI
BstEII BstPI Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MfeI MluI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI PshBI Psp1406I RsrII
SacII SalI SapI SbfI ScaI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2752 GACGTC GACGT^C
AcsI 1081 GAATTT [GA]^AATT[CT]
AflII 2851 CTTAAG C^TTAAG
AgeI 383 ACCGGT A^CCGGT
Aor51HI 1345 AGCGCT AGC^GCT
ApoI 1081 GAATTT [GA]^AATT[CT]
AspI 788 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3075 CCTAGG C^CTAGG
BbrPI 2767 CACGTG CAC^GTG
BciVI 2149 GGATAC GTATCC(6/5)
BfrI 2851 CTTAAG C^TTAAG
BlnI 3075 CCTAGG C^CTAGG
BsaAI 2767 CACGTG [CT]AC^GT[GA]
BsaBI 1327 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2331 GCATTC GAATGC(1/-1)
BspHI 2594 TCATGA T^CATGA
BspLU11I 1143 ACATGT A^CATGT
BsrBI 3316 GAGCGG CCGCTC(-3/-3)
BssSI 76 CTCGTG CACGAG(-5/-1)
Bst1107I 1780 GTATAC GTA^TAC
BstAPI 3024 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1780 GTATAC GTA^TAC
Eco47III 1345 AGCGCT AGC^GCT
MamI 1327 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 1143 ACATGT [GA]CATG^[CT]
PflFI 788 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 383 ACCGGT A^CCGGT
PmaCI 2767 CACGTG CAC^GTG
PmlI 2767 CACGTG CAC^GTG
PvuI 1191 CGATCG CGAT^CG
RcaI 2594 TCATGA T^CATGA
SacI 1363 GAGCTC GAGCT^C
SpeI 1784 ACTAGT A^CTAGT
TaqI 2900 TCGA T^CGA
Tth111I 788 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2900 TCGA T^CGA
XcmI 3449 CCATCTTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 473 GGTACC G^GTACC
501 GGTACC
AccI 1740 GTAGAC GT^[AC][GT]AC
1780 GTATAC
AccIII 2756 TCCGGA T^CCGGA
3488 TCCGGA
Asp718I 473 GGTACC G^GTACC
501 GGTACC
BbeI 2818 GGCGCC GGCGC^C
3519 GGCGCC
BbsI 1277 GAAGAC GAAGAC(2/6)
2458 GTCTTC
BcgI 703 GCAGGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1188 GCACGATCGTCG
BpuAI 1277 GAAGAC GAAGAC(2/6)
2458 GTCTTC
BsaI 2894 GAGACC GGTCTC(1/5)
3876 GAGACC
BseAI 2756 TCCGGA T^CCGGA
3488 TCCGGA
BsmBI 999 GAGACG CGTCTC(1/5)
3668 GAGACG
Bsp1407I 1118 TGTACA T^GTACA
2922 TGTACA
BspEI 2756 TCCGGA T^CCGGA
3488 TCCGGA
BsrDI 315 CATTGC GCAATG(2/0)
875 GCAATG
BsrGI 1118 TGTACA T^GTACA
2922 TGTACA
EagI 3530 CGGCCG C^GGCCG
3815 CGGCCG
EarI 3427 CTCTTC CTCTTC(1/4)
4007 GAAGAG
EclXI 3530 CGGCCG C^GGCCG
3815 CGGCCG
Eco52I 3530 CGGCCG C^GGCCG
3815 CGGCCG
EcoNI 1267 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1495 CCTGGTGCAGG
KasI 2818 GGCGCC G^GCGCC
3519 GGCGCC
KpnI 473 GGTACC GGTAC^C
501 GGTACC
Ksp632I 3427 CTCTTC CTCTTC(1/4)
4007 GAAGAG
MroI 2756 TCCGGA T^CCGGA
3488 TCCGGA
MslI 659 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4268 CACTGGGGTG
NarI 2818 GGCGCC GG^CGCC
3519 GGCGCC
SexAI 2465 ACCTGGT A^CC[AT]GGT
2723 ACCAGGT
SfoI 2818 GGCGCC GGC^GCC
3519 GGCGCC
SmlI 2851 CTTAAG C^T[CT][GA]AG
3262 CTCAAG
SspBI 1118 TGTACA T^GTACA
2922 TGTACA
Tsp509I 640 AATT ^AATT
1082 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI StuI
StyI TfiI Tru9I TseI Tsp45I
TspRI Van91I XhoII XmaCI XmaI