################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AviII AvrII BbsI BcgI BclI BfrI BlnI BpuAI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI Eco47III EcoRI EcoT22I FbaI FspI HindIII KpnI KspI MfeI MluI MroI MunI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SphI Sse8387I SwaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 1276 CGCG CG^CG AhdI 3059 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp700I 644 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 3059 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2287 GGATCC G^GATCC BbeI 2592 GGCGCC GGCGC^C BbvCI 3754 GCTGAGG CCTCAGC(-5/-2) BglI 4446 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 25 CGGCCG CG[GA][CT]^CG BsrBI 537 GAGCGG CCGCTC(-3/-3) BsrDI 614 GCAATG GCAATG(2/0) BstAPI 2354 GCACCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 521 GGTAACC G^GT[ACGT]ACC BstPI 521 GGTAACC G^GT[ACGT]ACC BstUI 1276 CGCG CG^CG DraI 577 TTTAAA TTT^AAA DsaI 1590 CCATGG C^C[GA][CT]GG EagI 25 CGGCCG C^GGCCG Eam1105I 3059 GACTGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 25 CGGCCG C^GGCCG Eco52I 25 CGGCCG C^GGCCG EcoO65I 521 GGTAACC G^GT[ACGT]ACC EcoRV 3568 GATATC GAT^ATC FseI 22 GGCCGGCC GGCCGG^CC HaeII 2592 GGCGCC [GA]GCGC^[CT] HincII 3517 GTTAAC GT[CT]^[GA]AC HindII 3517 GTTAAC GT[CT]^[GA]AC HpaI 3517 GTTAAC GTT^AAC KasI 2592 GGCGCC G^GCGCC MvnI 1276 CGCG CG^CG NarI 2592 GGCGCC GG^CGCC NcoI 1590 CCATGG C^CATGG NdeI 4325 CATATG CA^TATG PaeR7I 986 CTCGAG C^TCGAG PshAI 1193 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 2592 GGCGCC GGC^GCC SmaI 3466 CCCGGG CCC^GGG SpeI 4085 ACTAGT A^CTAGT SspI 4188 AATATT AAT^ATT XhoI 986 CTCGAG C^TCGAG XmaCI 3466 CCCGGG C^CCGGG XmaI 3466 CCCGGG C^CCGGG XmnI 644 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 2030 GAATTT [GA]^AATT[CT] 4098 GAATTT ApoI 2030 GAATTT [GA]^AATT[CT] 4098 GAATTT AspI 1525 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3146 GACAGGGTC BciVI 700 GGATAC GTATCC(6/5) 813 GTATCC BglII 1132 AGATCT A^GATCT 1561 AGATCT BsaBI 1138 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1160 GATATTGATC BsaWI 2922 ACCGGA [AT]^CCGG[AT] 3810 TCCGGT BsgI 1332 CTGCAC GTGCAG(16/14) 2006 GTGCAG BsmI 1420 GAATGC GAATGC(1/-1) 4156 GAATGC Bsp1407I 1623 TGTACA T^GTACA 3072 TGTACA BsrFI 23 GCCGGC [GA]^CCGG[CT] 27 GCCGGC BsrGI 1623 TGTACA T^GTACA 3072 TGTACA Cfr10I 23 GCCGGC [GA]^CCGG[CT] 27 GCCGGC HgaI 89 GACGC GACGC(5/10) 1712 GCGTC MamI 1138 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1160 GATATTGATC NaeI 23 GCCGGC GCC^GGC 27 GCCGGC NgoMIV 23 GCCGGC G^CCGGC 27 GCCGGC NspI 3698 GCATGT [GA]CATG^[CT] 3785 GCATGT PflFI 1525 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3146 GACAGGGTC PflMI 1100 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4022 CCAACAACTGG SspBI 1623 TGTACA T^GTACA 3072 TGTACA Tth111I 1525 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3146 GACAGGGTC Van91I 1100 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4022 CCAACAACTGG XbaI 4152 TCTAGA T^CTAGA 4306 TCTAGA XcmI 2649 CCATCCTGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2770 CCAGGAGGTGTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DrdI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII