##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AviII       AvrII       BbsI        BcgI        BclI        
BfrI        BlnI        BpuAI       BseAI       BsiWI       
BsmBI       BspDI       BspEI       BspHI       BspLU11I    
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
ClaI        CpoI        Eco47III    EcoRI       EcoT22I     
FbaI        FspI        HindIII     KpnI        KspI        
MfeI        MluI        MroI        MunI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccII        1276  CGCG                 CG^CG
AhdI         3059  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I       644  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3059  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        2287  GGATCC               G^GATCC
BbeI         2592  GGCGCC               GGCGC^C
BbvCI        3754  GCTGAGG              CCTCAGC(-5/-2)
BglI         4446  GCCAAGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI          25  CGGCCG               CG[GA][CT]^CG
BsrBI         537  GAGCGG               CCGCTC(-3/-3)
BsrDI         614  GCAATG               GCAATG(2/0)
BstAPI       2354  GCACCCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        521  GGTAACC              G^GT[ACGT]ACC
BstPI         521  GGTAACC              G^GT[ACGT]ACC
BstUI        1276  CGCG                 CG^CG
DraI          577  TTTAAA               TTT^AAA
DsaI         1590  CCATGG               C^C[GA][CT]GG
EagI           25  CGGCCG               C^GGCCG
Eam1105I     3059  GACTGCGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          25  CGGCCG               C^GGCCG
Eco52I         25  CGGCCG               C^GGCCG
EcoO65I       521  GGTAACC              G^GT[ACGT]ACC
EcoRV        3568  GATATC               GAT^ATC
FseI           22  GGCCGGCC             GGCCGG^CC
HaeII        2592  GGCGCC               [GA]GCGC^[CT]
HincII       3517  GTTAAC               GT[CT]^[GA]AC
HindII       3517  GTTAAC               GT[CT]^[GA]AC
HpaI         3517  GTTAAC               GTT^AAC
KasI         2592  GGCGCC               G^GCGCC
MvnI         1276  CGCG                 CG^CG
NarI         2592  GGCGCC               GG^CGCC
NcoI         1590  CCATGG               C^CATGG
NdeI         4325  CATATG               CA^TATG
PaeR7I        986  CTCGAG               C^TCGAG
PshAI        1193  GACTATAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI         2592  GGCGCC               GGC^GCC
SmaI         3466  CCCGGG               CCC^GGG
SpeI         4085  ACTAGT               A^CTAGT
SspI         4188  AATATT               AAT^ATT
XhoI          986  CTCGAG               C^TCGAG
XmaCI        3466  CCCGGG               C^CCGGG
XmaI         3466  CCCGGG               C^CCGGG
XmnI          644  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI         2030  GAATTT               [GA]^AATT[CT]
             4098  GAATTT              
ApoI         2030  GAATTT               [GA]^AATT[CT]
             4098  GAATTT              
AspI         1525  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3146  GACAGGGTC           
BciVI         700  GGATAC               GTATCC(6/5)
              813  GTATCC              
BglII        1132  AGATCT               A^GATCT
             1561  AGATCT              
BsaBI        1138  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1160  GATATTGATC          
BsaWI        2922  ACCGGA               [AT]^CCGG[AT]
             3810  TCCGGT              
BsgI         1332  CTGCAC               GTGCAG(16/14)
             2006  GTGCAG              
BsmI         1420  GAATGC               GAATGC(1/-1)
             4156  GAATGC              
Bsp1407I     1623  TGTACA               T^GTACA
             3072  TGTACA              
BsrFI          23  GCCGGC               [GA]^CCGG[CT]
               27  GCCGGC              
BsrGI        1623  TGTACA               T^GTACA
             3072  TGTACA              
Cfr10I         23  GCCGGC               [GA]^CCGG[CT]
               27  GCCGGC              
HgaI           89  GACGC                GACGC(5/10)
             1712  GCGTC               
MamI         1138  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1160  GATATTGATC          
NaeI           23  GCCGGC               GCC^GGC
               27  GCCGGC              
NgoMIV         23  GCCGGC               G^CCGGC
               27  GCCGGC              
NspI         3698  GCATGT               [GA]CATG^[CT]
             3785  GCATGT              
PflFI        1525  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3146  GACAGGGTC           
PflMI        1100  CCAGTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4022  CCAACAACTGG         
SspBI        1623  TGTACA               T^GTACA
             3072  TGTACA              
Tth111I      1525  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3146  GACAGGGTC           
Van91I       1100  CCAGTCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4022  CCAACAACTGG         
XbaI         4152  TCTAGA               T^CTAGA
             4306  TCTAGA              
XcmI         2649  CCATCCTGAATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2770  CCAGGAGGTGTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AocI        ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbrPI       
BbvI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DrdI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII