##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AcsI AflII AgeI ApoI
AscI AseI AsnI Asp700I BcgI
BclI BfrI BsiEI BsiWI BsmI
BspDI BspHI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoRI EcoT22I
FbaI HpaI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1062 GACGTC GACGT^C
Acc65I 1195 GGTACC G^GTACC
AccI 1608 GTCTAC GT^[AC][GT]AC
AccII 1826 CGCG CG^CG
AccIII 1115 TCCGGA T^CCGGA
AhdI 3355 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3660 GTGCAC G^TGCAC
Aor51HI 720 AGCGCT AGC^GCT
ApaLI 3660 GTGCAC G^TGCAC
Asp718I 1195 GGTACC G^GTACC
AspEI 3355 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3156 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1904 TGCGCA TGC^GCA
AvrII 3677 CCTAGG C^CTAGG
BamHI 4047 GGATCC G^GATCC
BciVI 2476 GGATAC GTATCC(6/5)
BglII 1387 AGATCT A^GATCT
BlnI 3677 CCTAGG C^CTAGG
BlpI 3845 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3845 GCTGAGC GC^T[ACGT]AGC
BsaBI 3422 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1115 TCCGGA T^CCGGA
Bsp1407I 2867 TGTACA T^GTACA
BspEI 1115 TCCGGA T^CCGGA
BsrBI 1761 GAGCGG CCGCTC(-3/-3)
BsrGI 2867 TGTACA T^GTACA
BstUI 1826 CGCG CG^CG
CelII 3845 GCTGAGC GC^T[ACGT]AGC
Eam1105I 3355 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 720 AGCGCT AGC^GCT
EcoRV 3682 GATATC GAT^ATC
FseI 3515 GGCCGGCC GGCCGG^CC
FspI 1904 TGCGCA TGC^GCA
HincII 3025 GTCAAC GT[CT]^[GA]AC
HindII 3025 GTCAAC GT[CT]^[GA]AC
KpnI 1195 GGTACC GGTAC^C
MamI 3422 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1115 TCCGGA T^CCGGA
MvnI 1826 CGCG CG^CG
NaeI 3516 GCCGGC GCC^GGC
NgoMIV 3516 GCCGGC G^CCGGC
PflFI 3156 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 3236 CCTGCAGG CCTGCA^GG
SexAI 1221 ACCTGGT A^CC[AT]GGT
SphI 1179 GCATGC GCATG^C
Sse8387I 3236 CCTGCAGG CCTGCA^GG
SspBI 2867 TGTACA T^GTACA
TaqI 3826 TCGA T^CGA
Tsp509I 4065 AATT ^AATT
Tth111I 3156 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3826 TCGA T^CGA
XbaI 473 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BbsI 3013 GAAGAC GAAGAC(2/6)
3305 GTCTTC
BglI 3067 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3507 GCCCAGCTGGC
BpuAI 3013 GAAGAC GAAGAC(2/6)
3305 GTCTTC
BsaWI 762 TCCGGT [AT]^CCGG[AT]
1115 TCCGGA
BsmBI 198 CGTCTC CGTCTC(1/5)
1091 CGTCTC
BsrDI 3431 CATTGC GCAATG(2/0)
4084 GCAATG
BsrFI 3472 ACCGGC [GA]^CCGG[CT]
3516 GCCGGC
BstAPI 3239 GCAGGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3258 GCAGCAGCTGC
BstEII 1085 GGTCACC G^GT[ACGT]ACC
2580 GGTCACC
BstPI 1085 GGTCACC G^GT[ACGT]ACC
2580 GGTCACC
Cfr10I 3472 ACCGGC [GA]^CCGG[CT]
3516 GCCGGC
DraIII 2434 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
4009 CACGCTGTG
DrdI 691 GACCCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3016 GACACCCAAGTC
EcoO65I 1085 GGTCACC G^GT[ACGT]ACC
2580 GGTCACC
MseI 108 TTAA T^TAA
215 TTAA
NspI 1179 GCATGC [GA]CATG^[CT]
2719 ACATGC
SacI 3983 GAGCTC GAGCT^C
4237 GAGCTC
ScaI 2388 AGTACT AGT^ACT
3637 AGTACT
SfaNI 623 GATGC GCATC(5/9)
1695 GATGC
SmaI 965 CCCGGG CCC^GGG
2085 CCCGGG
SmlI 2791 CTTGAG C^T[CT][GA]AG
3965 CTTGAG
TfiI 2288 GAATC G^A[AT]TC
3422 GATTC
Tru9I 108 TTAA T^TAA
215 TTAA
XmaCI 965 CCCGGG C^CCGGG
2085 CCCGGG
XmaI 965 CCCGGG C^CCGGG
2085 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcyI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbvCI BbvI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI SfoI
StuI StyI TseI Tsp45I TspRI
Van91I XcmI XhoII