##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BciVI BlnI BsaAI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BstEII BstPI ClaI DraI
DrdI Eam1105I Eco47III EcoO65I EcoT22I
FseI FspI HpaI MluI MroI
NheI NotI NruI NsiI PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I SexAI SfiI SgrAI
SnaBI SpeI SspI SwaI TfiI
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflII 4244 CTTAAG C^TTAAG
Asp700I 3967 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 1362 GGCGCC GGCGC^C
BcgI 2886 CGACTGGTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 679 TGATCA T^GATCA
BfrI 4244 CTTAAG C^TTAAG
BglII 2699 AGATCT A^GATCT
BsaBI 2289 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 4083 TGTACA T^GTACA
BspHI 184 TCATGA T^CATGA
BsrBI 3266 CCGCTC CCGCTC(-3/-3)
BsrGI 4083 TGTACA T^GTACA
Bst1107I 2264 GTATAC GTA^TAC
BstBI 2092 TTCGAA TT^CGAA
BstZ17I 2264 GTATAC GTA^TAC
CpoI 3146 CGGTCCG CG^G[AT]CCG
DraIII 4076 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 174 CGGCCG C^GGCCG
EclXI 174 CGGCCG C^GGCCG
Eco52I 174 CGGCCG C^GGCCG
EcoRV 539 GATATC GAT^ATC
FbaI 679 TGATCA T^GATCA
HgaI 353 GACGC GACGC(5/10)
HincII 2884 GTCGAC GT[CT]^[GA]AC
HindII 2884 GTCGAC GT[CT]^[GA]AC
HindIII 372 AAGCTT A^AGCTT
KasI 1362 GGCGCC G^GCGCC
KspI 35 CCGCGG CCGC^GG
MamI 2289 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 860 CAATTG C^AATTG
MunI 860 CAATTG C^AATTG
NaeI 4421 GCCGGC GCC^GGC
NarI 1362 GGCGCC GG^CGCC
NdeI 2789 CATATG CA^TATG
NgoMIV 4421 GCCGGC G^CCGGC
NspV 2092 TTCGAA TT^CGAA
PaeR7I 18 CTCGAG C^TCGAG
PflMI 1117 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 4416 CGATCG CGAT^CG
RcaI 184 TCATGA T^CATGA
RsrII 3146 CGGTCCG CG^G[AT]CCG
SacII 35 CCGCGG CCGC^GG
SalI 2884 GTCGAC G^TCGAC
ScaI 4282 AGTACT AGT^ACT
SfoI 1362 GGCGCC GGC^GCC
SfuI 2092 TTCGAA TT^CGAA
SspBI 4083 TGTACA T^GTACA
Van91I 1117 CCAGCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 18 CTCGAG C^TCGAG
XmnI 3967 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 512 GGTACC G^GTACC
3430 GGTACC
AccI 2264 GTATAC GT^[AC][GT]AC
2884 GTCGAC
AccII 36 CGCG CG^CG
1517 CGCG
AcsI 1537 GAATTC [GA]^AATT[CT]
4154 GAATTC
AcyI 353 GACGCC G[GA]^CG[CT]C
1362 GGCGCC
AocI 1693 CCTCAGG CC^T[ACGT]AGG
2300 CCTCAGG
ApoI 1537 GAATTC [GA]^AATT[CT]
4154 GAATTC
Asp718I 512 GGTACC G^GTACC
3430 GGTACC
BamHI 1570 GGATCC G^GATCC
3275 GGATCC
BglI 986 GCCTGTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3178 GCCGTGGTGGC
BlpI 1285 GCTCAGC GC^T[ACGT]AGC
2766 GCTGAGC
Bpu1102I 1285 GCTCAGC GC^T[ACGT]AGC
2766 GCTGAGC
BsaHI 353 GACGCC G[GA]^CG[CT]C
1362 GGCGCC
BsiEI 174 CGGCCG CG[GA][CT]^CG
4416 CGATCG
BsmBI 413 CGTCTC CGTCTC(1/5)
1233 GAGACG
BsrFI 73 GCCGGT [GA]^CCGG[CT]
4421 GCCGGC
BssSI 1461 CTCGTG CACGAG(-5/-1)
3628 CTCGTG
BstUI 36 CGCG CG^CG
1517 CGCG
Bsu36I 1693 CCTCAGG CC^T[ACGT]AGG
2300 CCTCAGG
CelII 1285 GCTCAGC GC^T[ACGT]AGC
2766 GCTGAGC
Cfr10I 73 GCCGGT [GA]^CCGG[CT]
4421 GCCGGC
Eco81I 1693 CCTCAGG CC^T[ACGT]AGG
2300 CCTCAGG
EcoRI 1537 GAATTC G^AATTC
4154 GAATTC
Hin1I 353 GACGCC G[GA]^CG[CT]C
1362 GGCGCC
KpnI 512 GGTACC GGTAC^C
3430 GGTACC
MaeII 2120 ACGT A^CGT
3695 ACGT
MvnI 36 CGCG CG^CG
1517 CGCG
PvuII 2688 CAGCTG CAG^CTG
3205 CAGCTG
SacI 866 GAGCTC GAGCT^C
2427 GAGCTC
SbfI 271 CCTGCAGG CCTGCA^GG
2919 CCTGCAGG
SmaI 1310 CCCGGG CCC^GGG
4502 CCCGGG
SphI 60 GCATGC GCATG^C
879 GCATGC
Sse8387I 271 CCTGCAGG CCTGCA^GG
2919 CCTGCAGG
XmaCI 1310 CCCGGG C^CCGGG
4502 CCCGGG
XmaI 1310 CCCGGG C^CCGGG
4502 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PleI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII