##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI Asp718I AviII BclI
BfrI BsaBI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI EagI EarI EclXI
Eco52I EcoO65I EcoRV FbaI FspI
HpaI KpnI Ksp632I KspI MamI
MfeI MroI MunI NotI NruI
NspV PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 418 GTAGAC GT^[AC][GT]AC
AcyI 603 GGCGCC G[GA]^CG[CT]C
Alw44I 4222 GTGCAC G^TGCAC
Aor51HI 4583 AGCGCT AGC^GCT
ApaI 4057 GGGCCC GGGCC^C
ApaLI 4222 GTGCAC G^TGCAC
AseI 2616 ATTAAT AT^TAAT
AsnI 2616 ATTAAT AT^TAAT
AspI 2316 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 33 CCTAGG C^CTAGG
BamHI 3299 GGATCC G^GATCC
BbeI 603 GGCGCC GGCGC^C
BglII 1929 AGATCT A^GATCT
BlnI 33 CCTAGG C^CTAGG
BsaHI 603 GGCGCC G[GA]^CG[CT]C
BsaWI 802 TCCGGT [AT]^CCGG[AT]
BsmI 690 GAATGC GAATGC(1/-1)
BspHI 1156 TCATGA T^CATGA
Eco47III 4583 AGCGCT AGC^GCT
EcoRI 3958 GAATTC G^AATTC
FseI 4317 GGCCGGCC GGCCGG^CC
Hin1I 603 GGCGCC G[GA]^CG[CT]C
HincII 3041 GTCAAC GT[CT]^[GA]AC
HindII 3041 GTCAAC GT[CT]^[GA]AC
KasI 603 GGCGCC G^GCGCC
MluI 3032 ACGCGT A^CGCGT
NarI 603 GGCGCC GG^CGCC
NcoI 4477 CCATGG C^CATGG
NdeI 1560 CATATG CA^TATG
NheI 4253 GCTAGC G^CTAGC
PacI 2613 TTAATTAA TTAAT^TAA
PaeR7I 4212 CTCGAG C^TCGAG
PflFI 2316 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4376 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 2616 ATTAAT AT^TAAT
RcaI 1156 TCATGA T^CATGA
SbfI 4290 CCTGCAGG CCTGCA^GG
SfoI 603 GGCGCC GGC^GCC
SmaI 2249 CCCGGG CCC^GGG
SpeI 1492 ACTAGT A^CTAGT
Sse8387I 4290 CCTGCAGG CCTGCA^GG
Tth111I 2316 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2966 CCAGATGGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4212 CTCGAG C^TCGAG
XmaCI 2249 CCCGGG C^CCGGG
XmaI 2249 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 2916 CGCG CG^CG
3033 CGCG
BciVI 1106 GGATAC GTATCC(6/5)
1569 GGATAC
BglI 430 GCCCTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1013 GCCCCTCAGGC
BsrDI 3056 CATTGC GCAATG(2/0)
2546 GCAATG
BstUI 2916 CGCG CG^CG
3033 CGCG
DsaI 295 CCGTGG C^C[GA][CT]GG
4477 CCATGG
EcoT22I 1234 ATGCAT ATGCA^T
2549 ATGCAT
HgaI 2358 GACGC GACGC(5/10)
3031 GACGC
HindIII 1979 AAGCTT A^AGCTT
3221 AAGCTT
MvnI 2916 CGCG CG^CG
3033 CGCG
NsiI 1234 ATGCAT ATGCA^T
2549 ATGCAT
PflMI 1068 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3277 CCAGTGGGTGG
PvuII 1365 CAGCTG CAG^CTG
1451 CAGCTG
SphI 529 GCATGC GCATG^C
2693 GCATGC
StuI 1618 AGGCCT AGG^CCT
2740 AGGCCT
TaqI 96 TCGA T^CGA
4213 TCGA
TthHB8I 96 TCGA T^CGA
4213 TCGA
Van91I 1068 CCATCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3277 CCAGTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AciI AcsI AfaI AflIII AhdI
AluI AlwI AlwNI AocI ApoI
Asp700I AspEI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EaeI Eam1105I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PmaCI
PmlI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XbaI XhoII XmnI