##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BfrI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII BstBI
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccII 1553 CGCG CG^CG
BamHI 3072 GGATCC G^GATCC
BciVI 39 GGATAC GTATCC(6/5)
BsaI 88 GAGACC GGTCTC(1/5)
BsmBI 2784 CGTCTC CGTCTC(1/5)
Bsp1407I 2596 TGTACA T^GTACA
BspHI 1622 TCATGA T^CATGA
BsrGI 2596 TGTACA T^GTACA
Bst1107I 1086 GTATAC GTA^TAC
BstEII 2373 GGTCACC G^GT[ACGT]ACC
BstPI 2373 GGTCACC G^GT[ACGT]ACC
BstUI 1553 CGCG CG^CG
BstYI 3072 GGATCC [GA]^GATC[CT]
BstZ17I 1086 GTATAC GTA^TAC
DraI 1593 TTTAAA TTT^AAA
DrdI 1398 GACCCCGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2373 GGTCACC G^GT[ACGT]ACC
EcoT22I 946 ATGCAT ATGCA^T
HindIII 293 AAGCTT A^AGCTT
MflI 3072 GGATCC [GA]^GATC[CT]
MvnI 1553 CGCG CG^CG
NaeI 3082 GCCGGC GCC^GGC
NgoMIV 3082 GCCGGC G^CCGGC
NsiI 946 ATGCAT ATGCA^T
NspI 368 GCATGC [GA]CATG^[CT]
PleI 1214 GAGTC GAGTC(4/5)
PshAI 3152 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1622 TCATGA T^CATGA
SphI 368 GCATGC GCATG^C
SspBI 2596 TGTACA T^GTACA
StuI 2414 AGGCCT AGG^CCT
TfiI 2863 GATTC G^A[AT]TC
XhoII 3072 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 501 GTCTAC GT^[AC][GT]AC
1086 GTATAC
AcsI 1105 AAATTT [GA]^AATT[CT]
3332 AAATTT
AcyI 2299 GACGCC G[GA]^CG[CT]C
3064 GGCGTC
AocI 2702 CCTCAGG CC^T[ACGT]AGG
3146 CCTCAGG
ApaI 381 GGGCCC GGGCC^C
2396 GGGCCC
ApoI 1105 AAATTT [GA]^AATT[CT]
3332 AAATTT
BbvCI 3011 CCTCAGC CCTCAGC(-5/-2)
1648 GCTGAGG
BclI 1223 TGATCA T^GATCA
1715 TGATCA
BlpI 640 GCTCAGC GC^T[ACGT]AGC
3033 GCTGAGC
Bpu1102I 640 GCTCAGC GC^T[ACGT]AGC
3033 GCTGAGC
BsaHI 2299 GACGCC G[GA]^CG[CT]C
3064 GGCGTC
BseRI 374 CTCCTC GAGGAG(10/8)
1651 GAGGAG
BsmI 315 GAATGC GAATGC(1/-1)
3025 GCATTC
BssSI 241 CTCGTG CACGAG(-5/-1)
2787 CTCGTG
BstXI 1310 CCATCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAAGTTCCTGG
Bsu36I 2702 CCTCAGG CC^T[ACGT]AGG
3146 CCTCAGG
CelII 640 GCTCAGC GC^T[ACGT]AGC
3033 GCTGAGC
Eco81I 2702 CCTCAGG CC^T[ACGT]AGG
3146 CCTCAGG
EcoNI 531 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2955 CCTTTTGGAGG
FbaI 1223 TGATCA T^GATCA
1715 TGATCA
Hin1I 2299 GACGCC G[GA]^CG[CT]C
3064 GGCGTC
HinfI 1214 GAGTC G^A[ACGT]TC
2863 GATTC
MaeII 74 ACGT A^CGT
1472 ACGT
NcoI 422 CCATGG C^CATGG
1331 CCATGG
SacI 1115 GAGCTC GAGCT^C
2233 GAGCTC
SapI 1767 GAAGAGC GCTCTTC(1/4)
3134 GAAGAGC
SmaI 221 CCCGGG CCC^GGG
384 CCCGGG
XcmI 179 CCAGCAAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
324 CCAGAGCCTGACTGG
XmaCI 221 CCCGGG C^CCGGG
384 CCCGGG
XmaI 221 CCCGGG C^CCGGG
384 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I