##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI BbeI
BbrPI BfrI BsaBI BsaHI BsaWI
BseAI BsiWI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV FseI HaeII
Hin1I KasI KpnI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 613 GTAGAC GT^[AC][GT]AC
AflIII 3512 ACATGT A^C[GA][CT]GT
Alw44I 2029 GTGCAC G^TGCAC
ApaLI 2029 GTGCAC G^TGCAC
Asp700I 1703 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2115 TGCGCA TGC^GCA
BamHI 1233 GGATCC G^GATCC
BanI 221 GGCACC G^G[CT][GA]CC
BglI 2608 GCCGTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2293 AGATCT A^GATCT
BsaI 1759 GGTCTC GGTCTC(1/5)
BsiEI 1643 CGGCCG CG[GA][CT]^CG
BsmBI 576 CGTCTC CGTCTC(1/5)
Bsp1407I 1955 TGTACA T^GTACA
BspHI 512 TCATGA T^CATGA
BspLU11I 3512 ACATGT A^CATGT
BsrDI 310 CATTGC GCAATG(2/0)
BsrGI 1955 TGTACA T^GTACA
DraI 953 TTTAAA TTT^AAA
DraII 3741 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 530 GACTGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1643 CGGCCG C^GGCCG
EarI 780 CTCTTC CTCTTC(1/4)
EclXI 1643 CGGCCG C^GGCCG
Eco52I 1643 CGGCCG C^GGCCG
EcoNI 3733 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3741 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1978 ATGCAT ATGCA^T
FspI 2115 TGCGCA TGC^GCA
HindIII 1942 AAGCTT A^AGCTT
Ksp632I 780 CTCTTC CTCTTC(1/4)
MfeI 4188 CAATTG C^AATTG
MunI 4188 CAATTG C^AATTG
NcoI 3273 CCATGG C^CATGG
NheI 160 GCTAGC G^CTAGC
NsiI 1978 ATGCAT ATGCA^T
RcaI 512 TCATGA T^CATGA
SmaI 4219 CCCGGG CCC^GGG
SphI 1976 GCATGC GCATG^C
SspBI 1955 TGTACA T^GTACA
XbaI 648 TCTAGA T^CTAGA
XcmI 2228 CCACTCCAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4219 CCCGGG C^CCGGG
XmaI 4219 CCCGGG C^CCGGG
XmnI 1703 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 305 CGCG CG^CG
3887 CGCG
AseI 1008 ATTAAT AT^TAAT
2275 ATTAAT
AsnI 1008 ATTAAT AT^TAAT
2275 ATTAAT
AspI 341 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4403 GACCCTGTC
AvrII 525 CCTAGG C^CTAGG
2451 CCTAGG
BanII 4197 GAGCCC G[GA]GC[CT]^C
4332 GAGCCC
BcgI 2513 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3491 CGAGTCTCCTGC
BciVI 490 GGATAC GTATCC(6/5)
588 GGATAC
BclI 2328 TGATCA T^GATCA
3838 TGATCA
BlnI 525 CCTAGG C^CTAGG
2451 CCTAGG
BsaAI 2135 CACGTA [CT]AC^GT[GA]
3597 CACGTA
BssSI 131 CACGAG CACGAG(-5/-1)
1173 CTCGTG
BstAPI 281 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2488 GCAACCTCTGC
BstUI 305 CGCG CG^CG
3887 CGCG
FbaI 2328 TGATCA T^GATCA
3838 TGATCA
HgaI 716 GACGC GACGC(5/10)
2565 GACGC
HpaI 1799 GTTAAC GTT^AAC
2150 GTTAAC
MvnI 305 CGCG CG^CG
3887 CGCG
PflFI 341 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4403 GACCCTGTC
PflMI 1632 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3442 CCAAATTATGG
PshBI 1008 ATTAAT AT^TAAT
2275 ATTAAT
SexAI 2053 ACCAGGT A^CC[AT]GGT
3357 ACCTGGT
Tth111I 341 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4403 GACCCTGTC
Van91I 1632 CCAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3442 CCAAATTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BalI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII