##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AviII BbeI BbrPI
BcgI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MunI
MvnI NarI NheI NotI NruI
NsiI NspV PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI TaqI Tth111I TthHB8I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1655 GTAGAC GT^[AC][GT]AC
AccIII 3769 TCCGGA T^CCGGA
AclI 1926 AACGTT AA^CGTT
AflII 2063 CTTAAG C^TTAAG
ApaI 1026 GGGCCC GGGCC^C
Asp700I 3670 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4252 CCCGAG C^[CT]CG[GA]G
AvrII 2455 CCTAGG C^CTAGG
BciVI 3783 GGATAC GTATCC(6/5)
BclI 3376 TGATCA T^GATCA
BfrI 2063 CTTAAG C^TTAAG
BlnI 2455 CCTAGG C^CTAGG
BseAI 3769 TCCGGA T^CCGGA
BsoBI 4252 CCCGAG C^[CT]CG[GA]G
BspEI 3769 TCCGGA T^CCGGA
BspLU11I 1659 ACATGT A^CATGT
BsrFI 332 GCCGGC [GA]^CCGG[CT]
Cfr10I 332 GCCGGC [GA]^CCGG[CT]
DraI 1623 TTTAAA TTT^AAA
FbaI 3376 TGATCA T^GATCA
HaeII 3520 AGCGCC [GA]GCGC^[CT]
MroI 3769 TCCGGA T^CCGGA
NaeI 332 GCCGGC GCC^GGC
NdeI 1797 CATATG CA^TATG
NgoMIV 332 GCCGGC G^CCGGC
PacI 1302 TTAATTAA TTAAT^TAA
Psp1406I 1926 AACGTT AA^CGTT
SapI 3759 GCTCTTC GCTCTTC(1/4)
SphI 4022 GCATGC GCATG^C
SspI 4423 AATATT AAT^ATT
XbaI 2802 TCTAGA T^CTAGA
XmnI 3670 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 1663 GTAC GT^AC
3052 GTAC
AflIII 978 ACGTGT A^C[GA][CT]GT
1659 ACATGT
BbvCI 2690 CCTCAGC CCTCAGC(-5/-2)
2284 GCTGAGG
BcnI 755 CCGGG CC^[GC]GG
3227 CCCGG
BlpI 963 GCTCAGC GC^T[ACGT]AGC
1139 GCTTAGC
Bpu1102I 963 GCTCAGC GC^T[ACGT]AGC
1139 GCTTAGC
BsaI 992 GAGACC GGTCTC(1/5)
2038 GAGACC
BsaWI 3769 TCCGGA [AT]^CCGG[AT]
4101 TCCGGT
BspHI 4307 TCATGA T^CATGA
4355 TCATGA
BsrBI 3638 CCGCTC CCGCTC(-3/-3)
1127 GAGCGG
BssSI 424 CACGAG CACGAG(-5/-1)
3679 CACGAG
BstXI 1034 CCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3328 CCAAGGGCCTGG
CelII 963 GCTCAGC GC^T[ACGT]AGC
1139 GCTTAGC
CfoI 98 GCGC GCG^C
3521 GCGC
EarI 3760 CTCTTC CTCTTC(1/4)
4359 GAAGAG
HhaI 98 GCGC GCG^C
3521 GCGC
HinP1I 98 GCGC G^CGC
3521 GCGC
HincII 345 GTTGAC GT[CT]^[GA]AC
3316 GTTGAC
HindII 345 GTTGAC GT[CT]^[GA]AC
3316 GTTGAC
HindIII 303 AAGCTT A^AGCTT
430 AAGCTT
Ksp632I 3760 CTCTTC CTCTTC(1/4)
4359 GAAGAG
NciI 755 CCGGG CC^[GC]GG
3227 CCCGG
NcoI 3932 CCATGG C^CATGG
3978 CCATGG
PstI 54 CTGCAG CTGCA^G
3613 CTGCAG
RcaI 4307 TCATGA T^CATGA
4355 TCATGA
RsaI 1663 GTAC GT^AC
3052 GTAC
SacI 1011 GAGCTC GAGCT^C
3628 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AluI Alw44I AlwI
AlwNI ApaLI ApoI AseI AsnI
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BfaI BglI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PshBI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII