##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AviII BamHI BbeI
BbrPI BciVI BfrI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstUI BstZ17I Cfr10I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
KasI KpnI KspI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccIII 1595 TCCGGA T^CCGGA
AcsI 301 AAATTT [GA]^AATT[CT]
AcyI 1651 GACGCC G[GA]^CG[CT]C
Alw44I 4002 GTGCAC G^TGCAC
ApaLI 4002 GTGCAC G^TGCAC
ApoI 301 AAATTT [GA]^AATT[CT]
Asp700I 4413 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2532 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 89 CCTAGG C^CTAGG
BcgI 3582 GCAATGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 89 CCTAGG C^CTAGG
BsaAI 1771 TACGTG [CT]AC^GT[GA]
BsaBI 367 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1651 GACGCC G[GA]^CG[CT]C
BseAI 1595 TCCGGA T^CCGGA
BsmI 2295 GAATGC GAATGC(1/-1)
BspEI 1595 TCCGGA T^CCGGA
DraIII 3796 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
HaeII 286 AGCGCC [GA]GCGC^[CT]
Hin1I 1651 GACGCC G[GA]^CG[CT]C
HpaI 3964 GTTAAC GTT^AAC
MamI 367 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1595 TCCGGA T^CCGGA
NcoI 986 CCATGG C^CATGG
NheI 1865 GCTAGC G^CTAGC
PflFI 2532 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1314 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 1960 GTCGAC G^TCGAC
SapI 1542 GCTCTTC GCTCTTC(1/4)
SexAI 2533 ACCTGGT A^CC[AT]GGT
SpeI 3566 ACTAGT A^CTAGT
SphI 2993 GCATGC GCATG^C
SspI 4159 AATATT AAT^ATT
Tth111I 2532 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1314 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2554 CCACCCTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4413 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1960 GTCGAC GT^[AC][GT]AC
4290 GTAGAC
AhdI 753 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3212 GACCCTGAGTC
AspEI 753 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3212 GACCCTGAGTC
BclI 1553 TGATCA T^GATCA
2814 TGATCA
BglI 2091 GCCACTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3441 GCCAGCTGGGC
BglII 727 AGATCT A^GATCT
2195 AGATCT
BsaWI 19 ACCGGA [AT]^CCGG[AT]
1595 TCCGGA
BstBI 784 TTCGAA TT^CGAA
2877 TTCGAA
DrdI 1048 GACCTGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1951 GACTGAGGGGTC
Eam1105I 753 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3212 GACCCTGAGTC
FbaI 1553 TGATCA T^GATCA
2814 TGATCA
HgaI 1651 GACGC GACGC(5/10)
1678 GCGTC
HincII 1960 GTCGAC GT[CT]^[GA]AC
3964 GTTAAC
HindII 1960 GTCGAC GT[CT]^[GA]AC
3964 GTTAAC
NspI 25 GCATGT [GA]CATG^[CT]
2993 GCATGC
NspV 784 TTCGAA TT^CGAA
2877 TTCGAA
SfuI 784 TTCGAA TT^CGAA
2877 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrDI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII