##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AflII AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BcgI BclI BfrI
BglI BlnI BsiEI BsiWI Bsp1407I
BspHI BsrBI BsrFI BsrGI BssHII
Bst1107I BstUI BstZ17I Cfr10I CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I FbaI FseI FspI
HgaI KpnI KspI MluI MvnI
NaeI NgoMIV NheI NotI NruI
PaeR7I PinAI PmeI PshAI PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 3305 GTAGAC GT^[AC][GT]AC
AccIII 2421 TCCGGA T^CCGGA
AclI 4638 AACGTT AA^CGTT
AcyI 501 GGCGCC G[GA]^CG[CT]C
Alw44I 994 GTGCAC G^TGCAC
AocI 3585 CCTGAGG CC^T[ACGT]AGG
ApaI 3560 GGGCCC GGGCC^C
ApaLI 994 GTGCAC G^TGCAC
AseI 3851 ATTAAT AT^TAAT
AsnI 3851 ATTAAT AT^TAAT
BbeI 501 GGCGCC GGCGC^C
BbrPI 2820 CACGTG CAC^GTG
BbvCI 529 GCTGAGG CCTCAGC(-5/-2)
BciVI 2424 GGATAC GTATCC(6/5)
BlpI 4182 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4182 GCTCAGC GC^T[ACGT]AGC
BsaBI 4194 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 501 GGCGCC G[GA]^CG[CT]C
BsaWI 2421 TCCGGA [AT]^CCGG[AT]
BseAI 2421 TCCGGA T^CCGGA
BsmBI 458 CGTCTC CGTCTC(1/5)
BspDI 2867 ATCGAT AT^CGAT
BspEI 2421 TCCGGA T^CCGGA
BssSI 411 CACGAG CACGAG(-5/-1)
BstBI 4415 TTCGAA TT^CGAA
BstEII 2734 GGTTACC G^GT[ACGT]ACC
BstPI 2734 GGTTACC G^GT[ACGT]ACC
Bsu36I 3585 CCTGAGG CC^T[ACGT]AGG
CelII 4182 GCTCAGC GC^T[ACGT]AGC
CfoI 502 GCGC GCG^C
ClaI 2867 ATCGAT AT^CGAT
DraIII 4428 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3585 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2734 GGTTACC G^GT[ACGT]ACC
EcoRI 2642 GAATTC G^AATTC
EcoRV 2551 GATATC GAT^ATC
FauI 401 GCGGG CCCGC(4/6)
HaeII 501 GGCGCC [GA]GCGC^[CT]
HhaI 502 GCGC GCG^C
Hin1I 501 GGCGCC G[GA]^CG[CT]C
HinP1I 502 GCGC G^CGC
HindIII 1537 AAGCTT A^AGCTT
HpaI 2633 GTTAAC GTT^AAC
KasI 501 GGCGCC G^GCGCC
MamI 4194 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1745 CAATTG C^AATTG
MroI 2421 TCCGGA T^CCGGA
MspA1I 949 CAGCTG C[AC]G^C[GT]G
MunI 1745 CAATTG C^AATTG
NarI 501 GGCGCC GG^CGCC
NcoI 2041 CCATGG C^CATGG
NspV 4415 TTCGAA TT^CGAA
PmaCI 2820 CACGTG CAC^GTG
PmlI 2820 CACGTG CAC^GTG
PshBI 3851 ATTAAT AT^TAAT
Psp1406I 4638 AACGTT AA^CGTT
PvuII 949 CAGCTG CAG^CTG
ScaI 3544 AGTACT AGT^ACT
SfoI 501 GGCGCC GGC^GCC
SfuI 4415 TTCGAA TT^CGAA
SmaI 553 CCCGGG CCC^GGG
SphI 2476 GCATGC GCATG^C
XmaCI 553 CCCGGG C^CCGGG
XmaI 553 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 836 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4259 GAACCACTTC
AspI 620 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2373 GACTTAGTC
AvaI 553 CCCGGG C^[CT]CG[GA]G
666 CCCGAG
BalI 326 TGGCCA TGG^CCA
3016 TGGCCA
BglII 3142 AGATCT A^GATCT
3166 AGATCT
BsoBI 553 CCCGGG C^[CT]CG[GA]G
666 CCCGAG
BspLU11I 3818 ACATGT A^CATGT
4680 ACATGT
BstAPI 2183 GCAAATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4558 GCAGTGACTGC
EcoT22I 1240 ATGCAT ATGCA^T
4352 ATGCAT
MluNI 326 TGGCCA TGG^CCA
3016 TGGCCA
MscI 326 TGGCCA TGG^CCA
3016 TGGCCA
NsiI 1240 ATGCAT ATGCA^T
4352 ATGCAT
PacI 3848 TTAATTAA TTAAT^TAA
3852 TTAATTAA
PflFI 620 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2373 GACTTAGTC
SspI 1806 AATATT AAT^ATT
2186 AATATT
Tth111I 620 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2373 GACTTAGTC
XmnI 836 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4259 GAACCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII