##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AseI AsnI Asp700I
AviII BglII BsaBI BsiWI BspDI
BspHI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EcoRI EcoRV EcoT22I
FseI FspI HpaI MamI MfeI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 4244 GGTACC G^GTACC
AccIII 3743 TCCGGA T^CCGGA
AcsI 2102 AAATTC [GA]^AATT[CT]
AgeI 694 ACCGGT A^CCGGT
Alw44I 1716 GTGCAC G^TGCAC
AocI 3310 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1028 AGCGCT AGC^GCT
ApaLI 1716 GTGCAC G^TGCAC
ApoI 2102 AAATTC [GA]^AATT[CT]
AscI 384 GGCGCGCC GG^CGCGCC
Asp718I 4244 GGTACC G^GTACC
AspI 3772 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3350 CCTAGG C^CTAGG
BbrPI 340 CACGTG CAC^GTG
BcgI 1660 GCACGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1405 GGATAC GTATCC(6/5)
BclI 2368 TGATCA T^GATCA
BglI 338 GCCACGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3350 CCTAGG C^CTAGG
BseAI 3743 TCCGGA T^CCGGA
BspEI 3743 TCCGGA T^CCGGA
BstEII 1899 GGTCACC G^GT[ACGT]ACC
BstPI 1899 GGTCACC G^GT[ACGT]ACC
Bsu36I 3310 CCTCAGG CC^T[ACGT]AGG
Eco47III 1028 AGCGCT AGC^GCT
Eco81I 3310 CCTCAGG CC^T[ACGT]AGG
EcoNI 4227 CCTGCTTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1899 GGTCACC G^GT[ACGT]ACC
FbaI 2368 TGATCA T^GATCA
HindIII 2599 AAGCTT A^AGCTT
KpnI 4244 GGTACC GGTAC^C
MroI 3743 TCCGGA T^CCGGA
NaeI 1115 GCCGGC GCC^GGC
NgoMIV 1115 GCCGGC G^CCGGC
PflFI 3772 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 694 ACCGGT A^CCGGT
PmaCI 340 CACGTG CAC^GTG
PmlI 340 CACGTG CAC^GTG
SacI 2543 GAGCTC GAGCT^C
SalI 760 GTCGAC G^TCGAC
ScaI 4253 AGTACT AGT^ACT
SmaI 2349 CCCGGG CCC^GGG
Tth111I 3772 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2349 CCCGGG C^CCGGG
XmaI 2349 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 760 GTCGAC GT^[AC][GT]AC
4020 GTCTAC
AhdI 1186 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3508 GACATGCTGTC
ApaI 661 GGGCCC GGGCC^C
3115 GGGCCC
AspEI 1186 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3508 GACATGCTGTC
BamHI 1779 GGATCC G^GATCC
3867 GGATCC
BbeI 355 GGCGCC GGCGC^C
945 GGCGCC
BbsI 2826 GAAGAC GAAGAC(2/6)
3791 GAAGAC
BlpI 166 GCTGAGC GC^T[ACGT]AGC
3360 GCTGAGC
Bpu1102I 166 GCTGAGC GC^T[ACGT]AGC
3360 GCTGAGC
BpuAI 2826 GAAGAC GAAGAC(2/6)
3791 GAAGAC
BsaWI 694 ACCGGT [AT]^CCGG[AT]
3743 TCCGGA
BsmBI 1395 CGTCTC CGTCTC(1/5)
4108 GAGACG
BsmI 674 GAATGC GAATGC(1/-1)
2618 GAATGC
Bsp1407I 1301 TGTACA T^GTACA
4007 TGTACA
BsrBI 73 GAGCGG CCGCTC(-3/-3)
370 GAGCGG
BsrFI 694 ACCGGT [GA]^CCGG[CT]
1115 GCCGGC
BsrGI 1301 TGTACA T^GTACA
4007 TGTACA
BstAPI 87 GCACGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
111 GCAGCTGCTGC
CelII 166 GCTGAGC GC^T[ACGT]AGC
3360 GCTGAGC
Cfr10I 694 ACCGGT [GA]^CCGG[CT]
1115 GCCGGC
DraIII 88 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1101 CACTGCGTG
DrdI 745 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
867 GACGGGTGGGTC
EagI 206 CGGCCG C^GGCCG
1612 CGGCCG
Eam1105I 1186 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3508 GACATGCTGTC
EclXI 206 CGGCCG C^GGCCG
1612 CGGCCG
Eco52I 206 CGGCCG C^GGCCG
1612 CGGCCG
HincII 760 GTCGAC GT[CT]^[GA]AC
4179 GTTGAC
HindII 760 GTCGAC GT[CT]^[GA]AC
4179 GTTGAC
KasI 355 GGCGCC G^GCGCC
945 GGCGCC
MluI 2011 ACGCGT A^CGCGT
4111 ACGCGT
NarI 355 GGCGCC GG^CGCC
945 GGCGCC
NcoI 1082 CCATGG C^CATGG
1154 CCATGG
SapI 1857 GAAGAGC GCTCTTC(1/4)
3052 GCTCTTC
SfoI 355 GGCGCC GGC^GCC
945 GGCGCC
SspBI 1301 TGTACA T^GTACA
4007 TGTACA
Tsp509I 2103 AATT ^AATT
4032 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AflII
AflIII AluI AlwI AlwNI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BfrI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssHII BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII