##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AflII Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspI AvrII BamHI BclI BfrI
BlnI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrGI Bst1107I BstZ17I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NotI NruI NsiI PacI
PflFI PmeI PshBI Psp1406I PvuI
RsrII SapI ScaI SexAI SgrAI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3116 GACGTC GACGT^C
Acc65I 1980 GGTACC G^GTACC
AcsI 2262 AAATTC [GA]^AATT[CT]
AgeI 2242 ACCGGT A^CCGGT
AhdI 3945 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1441 CCTCAGG CC^T[ACGT]AGG
ApoI 2262 AAATTC [GA]^AATT[CT]
Asp718I 1980 GGTACC G^GTACC
AspEI 3945 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3265 TGCGCA TGC^GCA
BalI 2630 TGGCCA TGG^CCA
BbeI 4236 GGCGCC GGCGC^C
BciVI 632 GTATCC GTATCC(6/5)
BglII 1228 AGATCT A^GATCT
BssHII 2984 GCGCGC G^CGCGC
BstBI 3042 TTCGAA TT^CGAA
BstEII 2245 GGTCACC G^GT[ACGT]ACC
BstPI 2245 GGTCACC G^GT[ACGT]ACC
Bsu36I 1441 CCTCAGG CC^T[ACGT]AGG
DrdI 2861 GACACCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3945 GACTCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1441 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2245 GGTCACC G^GT[ACGT]ACC
FspI 3265 TGCGCA TGC^GCA
KasI 4236 GGCGCC G^GCGCC
KpnI 1980 GGTACC GGTAC^C
MluNI 2630 TGGCCA TGG^CCA
MscI 2630 TGGCCA TGG^CCA
NarI 4236 GGCGCC GG^CGCC
NheI 2159 GCTAGC G^CTAGC
NspV 3042 TTCGAA TT^CGAA
PaeR7I 3721 CTCGAG C^TCGAG
PinAI 2242 ACCGGT A^CCGGT
PshAI 2388 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 4236 GGCGCC GGC^GCC
SfuI 3042 TTCGAA TT^CGAA
SnaBI 480 TACGTA TAC^GTA
XhoI 3721 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2325 GTCGAC GT^[AC][GT]AC
2578 GTCGAC
BbrPI 100 CACGTG CAC^GTG
4401 CACGTG
BbsI 638 GTCTTC GAAGAC(2/6)
3012 GTCTTC
BcgI 3591 CGATGCCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
865 GCACTGAGGTCG
BpuAI 638 GTCTTC GAAGAC(2/6)
3012 GTCTTC
BsaWI 2022 ACCGGA [AT]^CCGG[AT]
2242 ACCGGT
BspHI 1222 TCATGA T^CATGA
4379 TCATGA
BspLU11I 1542 ACATGT A^CATGT
2321 ACATGT
BsrDI 1280 CATTGC GCAATG(2/0)
2850 CATTGC
BstAPI 2690 GCATTAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4469 GCAGTGGCTGC
HincII 2325 GTCGAC GT[CT]^[GA]AC
2578 GTCGAC
HindII 2325 GTCGAC GT[CT]^[GA]AC
2578 GTCGAC
MseI 1722 TTAA T^TAA
2693 TTAA
PmaCI 100 CACGTG CAC^GTG
4401 CACGTG
PmlI 100 CACGTG CAC^GTG
4401 CACGTG
RcaI 1222 TCATGA T^CATGA
4379 TCATGA
SacI 274 GAGCTC GAGCT^C
4077 GAGCTC
SalI 2325 GTCGAC G^TCGAC
2578 GTCGAC
SbfI 2109 CCTGCAGG CCTGCA^GG
3317 CCTGCAGG
SfiI 2072 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4040 GGCCGCGGGGGCC
Sse8387I 2109 CCTGCAGG CCTGCA^GG
3317 CCTGCAGG
Tru9I 1722 TTAA T^TAA
2693 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI