##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AocI AscI AseI AsnI Asp718I
AspI AvrII BclI BfaI BfrI
BlnI BsaBI BsiWI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
EclXI Eco52I Eco81I EcoT22I FbaI
FseI KpnI MaeI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AatII 1671 GACGTC GACGT^C
AhdI 3259 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1865 GTGCAC G^TGCAC
ApaLI 1865 GTGCAC G^TGCAC
Asp700I 251 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3259 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 928 GGATCC G^GATCC
BciVI 3448 GGATAC GTATCC(6/5)
BsmI 3998 GCATTC GAATGC(1/-1)
BspLU11I 412 ACATGT A^CATGT
BstAPI 1577 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 4165 TTTAAA TTT^AAA
Eam1105I 3259 GACACATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3155 GAATTC G^AATTC
EcoRV 561 GATATC GAT^ATC
HpaI 3859 GTTAAC GTT^AAC
NcoI 136 CCATGG C^CATGG
NdeI 4003 CATATG CA^TATG
PaeR7I 588 CTCGAG C^TCGAG
SacI 2900 GAGCTC GAGCT^C
SapI 1763 GCTCTTC GCTCTTC(1/4)
SfiI 3392 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3189 GCATGC GCATG^C
XhoI 588 CTCGAG C^TCGAG
XmnI 251 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 412 ACATGT A^C[GA][CT]GT
1525 ACGTGT
AviII 616 TGCGCA TGC^GCA
1984 TGCGCA
BbeI 1798 GGCGCC GGCGC^C
2251 GGCGCC
BbrPI 2256 CACGTG CAC^GTG
4101 CACGTG
BbvCI 2606 CCTCAGC CCTCAGC(-5/-2)
4085 GCTGAGG
BlpI 1490 GCTGAGC GC^T[ACGT]AGC
3316 GCTGAGC
Bpu1102I 1490 GCTGAGC GC^T[ACGT]AGC
3316 GCTGAGC
BsmBI 1699 GAGACG CGTCTC(1/5)
2055 GAGACG
BspHI 817 TCATGA T^CATGA
1507 TCATGA
BsrDI 233 CATTGC GCAATG(2/0)
3001 GCAATG
BstEII 2438 GGTCACC G^GT[ACGT]ACC
3588 GGTCACC
BstPI 2438 GGTCACC G^GT[ACGT]ACC
3588 GGTCACC
CelII 1490 GCTGAGC GC^T[ACGT]AGC
3316 GCTGAGC
DraIII 171 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
1944 CACCAGGTG
EcoO65I 2438 GGTCACC G^GT[ACGT]ACC
3588 GGTCACC
FspI 616 TGCGCA TGC^GCA
1984 TGCGCA
HincII 2115 GTCAAC GT[CT]^[GA]AC
3859 GTTAAC
HindII 2115 GTCAAC GT[CT]^[GA]AC
3859 GTTAAC
HindIII 2293 AAGCTT A^AGCTT
4017 AAGCTT
KasI 1798 GGCGCC G^GCGCC
2251 GGCGCC
KspI 2064 CCGCGG CCGC^GG
2822 CCGCGG
NarI 1798 GGCGCC GG^CGCC
2251 GGCGCC
PmaCI 2256 CACGTG CAC^GTG
4101 CACGTG
PmlI 2256 CACGTG CAC^GTG
4101 CACGTG
RcaI 817 TCATGA T^CATGA
1507 TCATGA
SacII 2064 CCGCGG CCGC^GG
2822 CCGCGG
ScaI 646 AGTACT AGT^ACT
1447 AGTACT
SfoI 1798 GGCGCC GGC^GCC
2251 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AfaI AluI AlwI AlwNI Aor51HI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BglI BglII BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI