##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI ApoI AscI AseI AsnI
BamHI BbsI BciVI BclI BfrI
BglII BpuAI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EcoRI EcoRV FbaI
FseI HindIII HpaI MamI MluI
MroI NaeI NgoMIV NheI NotI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SapI ScaI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 2927 GGTACC G^GTACC
AflIII 411 ACATGT A^C[GA][CT]GT
Aor51HI 3386 AGCGCT AGC^GCT
Asp700I 3900 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2927 GGTACC G^GTACC
AspI 1798 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3545 TGCGCA TGC^GCA
BcgI 4206 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 2768 CGGCCG CG[GA][CT]^CG
BspHI 111 TCATGA T^CATGA
BspLU11I 411 ACATGT A^CATGT
BsrDI 2168 CATTGC GCAATG(2/0)
BssSI 1308 CACGAG CACGAG(-5/-1)
BstEII 2631 GGTCACC G^GT[ACGT]ACC
BstPI 2631 GGTCACC G^GT[ACGT]ACC
DraIII 972 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2768 CGGCCG C^GGCCG
EclXI 2768 CGGCCG C^GGCCG
Eco47III 3386 AGCGCT AGC^GCT
Eco52I 2768 CGGCCG C^GGCCG
EcoO65I 2631 GGTCACC G^GT[ACGT]ACC
EcoT22I 430 ATGCAT ATGCA^T
FspI 3545 TGCGCA TGC^GCA
HincII 3020 GTCGAC GT[CT]^[GA]AC
HindII 3020 GTCGAC GT[CT]^[GA]AC
KpnI 2927 GGTACC GGTAC^C
MfeI 2250 CAATTG C^AATTG
MunI 2250 CAATTG C^AATTG
NdeI 1851 CATATG CA^TATG
NruI 2935 TCGCGA TCG^CGA
NsiI 430 ATGCAT ATGCA^T
PflFI 1798 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 111 TCATGA T^CATGA
SacI 3978 GAGCTC GAGCT^C
SalI 3020 GTCGAC G^TCGAC
SexAI 973 ACCTGGT A^CC[AT]GGT
SfiI 3102 GGCCCTGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tth111I 1798 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 305 CCAAATGCACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3900 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 874 GTAGAC GT^[AC][GT]AC
3020 GTCGAC
AhdI 1930 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2442 GACAGGGCGTC
AspEI 1930 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2442 GACAGGGCGTC
AvrII 1507 CCTAGG C^CTAGG
4224 CCTAGG
BbrPI 3325 CACGTG CAC^GTG
3616 CACGTG
BlnI 1507 CCTAGG C^CTAGG
4224 CCTAGG
BsmBI 63 GAGACG CGTCTC(1/5)
2428 GAGACG
Bsp1407I 535 TGTACA T^GTACA
3188 TGTACA
BsrBI 3147 CCGCTC CCGCTC(-3/-3)
3625 CCGCTC
BsrGI 535 TGTACA T^GTACA
3188 TGTACA
BstXI 135 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4050 CCAGCACTTTGG
BstYI 841 GGATCT [GA]^GATC[CT]
1046 AGATCC
Eam1105I 1930 GACCCGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2442 GACAGGGCGTC
KspI 3158 CCGCGG CCGC^GG
3516 CCGCGG
MflI 841 GGATCT [GA]^GATC[CT]
1046 AGATCC
NcoI 3633 CCATGG C^CATGG
3648 CCATGG
NspI 411 ACATGT [GA]CATG^[CT]
428 ACATGC
PmaCI 3325 CACGTG CAC^GTG
3616 CACGTG
PmlI 3325 CACGTG CAC^GTG
3616 CACGTG
SacII 3158 CCGCGG CCGC^GG
3516 CCGCGG
SbfI 1150 CCTGCAGG CCTGCA^GG
3105 CCTGCAGG
SmlI 1534 CTCAAG C^T[CT][GA]AG
2269 CTCAAG
Sse8387I 1150 CCTGCAGG CCTGCA^GG
3105 CCTGCAGG
SspBI 535 TGTACA T^GTACA
3188 TGTACA
StuI 1052 AGGCCT AGG^CCT
2531 AGGCCT
XhoII 841 GGATCT [GA]^GATC[CT]
1046 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XmaCI
XmaI