##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AflII AhdI Aor51HI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BbsI BcgI BfrI
BpuAI BsaAI BsiEI BsiWI BspDI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI HaeII HpaI KasI KspI
MluI NaeI NarI NgoMIV NotI
NruI NspV PacI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 4167 GGTACC G^GTACC
AccI 2835 GTCTAC GT^[AC][GT]AC
AccII 1800 CGCG CG^CG
AccIII 2170 TCCGGA T^CCGGA
AcyI 2154 GGCGTC G[GA]^CG[CT]C
AgeI 1614 ACCGGT A^CCGGT
Asp718I 4167 GGTACC G^GTACC
AspI 4230 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2192 CCTCAGC CCTCAGC(-5/-2)
BclI 1161 TGATCA T^GATCA
BglI 2578 GCCGGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4441 AGATCT A^GATCT
BlpI 1845 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1845 GCTCAGC GC^T[ACGT]AGC
BsaHI 2154 GGCGTC G[GA]^CG[CT]C
BseAI 2170 TCCGGA T^CCGGA
BsmBI 2219 CGTCTC CGTCTC(1/5)
BspEI 2170 TCCGGA T^CCGGA
BspHI 1624 TCATGA T^CATGA
BsrDI 3609 GCAATG GCAATG(2/0)
BsrFI 1614 ACCGGT [GA]^CCGG[CT]
BstUI 1800 CGCG CG^CG
CelII 1845 GCTCAGC GC^T[ACGT]AGC
CfoI 1747 GCGC GCG^C
Cfr10I 1614 ACCGGT [GA]^CCGG[CT]
DraI 1054 TTTAAA TTT^AAA
FbaI 1161 TGATCA T^GATCA
HhaI 1747 GCGC GCG^C
Hin1I 2154 GGCGTC G[GA]^CG[CT]C
HinP1I 1747 GCGC G^CGC
HindIII 1175 AAGCTT A^AGCTT
KpnI 4167 GGTACC GGTAC^C
MfeI 2651 CAATTG C^AATTG
MroI 2170 TCCGGA T^CCGGA
MunI 2651 CAATTG C^AATTG
MvnI 1800 CGCG CG^CG
NheI 371 GCTAGC G^CTAGC
PaeR7I 1896 CTCGAG C^TCGAG
PflFI 4230 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3403 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1614 ACCGGT A^CCGGT
PshAI 115 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1624 TCATGA T^CATGA
ScaI 2508 AGTACT AGT^ACT
SexAI 214 ACCAGGT A^CC[AT]GGT
SmaI 1538 CCCGGG CCC^GGG
SphI 3294 GCATGC GCATG^C
TaqI 1897 TCGA T^CGA
Tth111I 4230 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1897 TCGA T^CGA
Van91I 3403 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 656 TCTAGA T^CTAGA
XcmI 3748 CCACCTGAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1896 CTCGAG C^TCGAG
XmaCI 1538 CCCGGG C^CCGGG
XmaI 1538 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Asp700I 3125 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3143 GAAGGGCTTC
AvrII 1639 CCTAGG C^CTAGG
3059 CCTAGG
BamHI 2173 GGATCC G^GATCC
3184 GGATCC
BciVI 304 GTATCC GTATCC(6/5)
899 GTATCC
BlnI 1639 CCTAGG C^CTAGG
3059 CCTAGG
BsaBI 1252 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3637 GATATCCATC
BsmI 2360 GAATGC GAATGC(1/-1)
3960 GCATTC
BstXI 973 CCAAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2845 CCAAGTAAGTGG
EcoRV 787 GATATC GAT^ATC
3637 GATATC
FauI 1930 CCCGC CCCGC(4/6)
1867 GCGGG
HincII 1480 GTTGAC GT[CT]^[GA]AC
2157 GTCAAC
HindII 1480 GTTGAC GT[CT]^[GA]AC
2157 GTCAAC
MaeII 2218 ACGT A^CGT
3942 ACGT
MamI 1252 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3637 GATATCCATC
MspA1I 2488 CAGCTG C[AC]G^C[GT]G
3426 CAGCTG
NcoI 3330 CCATGG C^CATGG
3757 CCATGG
NdeI 1123 CATATG CA^TATG
4382 CATATG
PvuII 2488 CAGCTG CAG^CTG
3426 CAGCTG
SapI 1993 GAAGAGC GCTCTTC(1/4)
4355 GCTCTTC
XmnI 3125 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3143 GAAGGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspLU11I BspMI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII