##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AclI AhdI AscI AseI
AsnI AspEI AviII BamHI BbrPI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoRI EcoRV
EcoT22I FspI HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 3953 GGTACC G^GTACC
AccI 1592 GTAGAC GT^[AC][GT]AC
AccIII 1093 TCCGGA T^CCGGA
AcsI 4041 AAATTC [GA]^AATT[CT]
AflII 2526 CTTAAG C^TTAAG
AgeI 2105 ACCGGT A^CCGGT
Aor51HI 3368 AGCGCT AGC^GCT
ApoI 4041 AAATTC [GA]^AATT[CT]
Asp700I 3120 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3953 GGTACC G^GTACC
AspI 2919 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2341 CCTAGG C^CTAGG
BbeI 1613 GGCGCC GGCGC^C
BclI 1062 TGATCA T^GATCA
BfrI 2526 CTTAAG C^TTAAG
BglII 3347 AGATCT A^GATCT
BlnI 2341 CCTAGG C^CTAGG
BsaAI 1556 TACGTG [CT]AC^GT[GA]
BseAI 1093 TCCGGA T^CCGGA
BsiEI 889 CGACCG CG[GA][CT]^CG
BspEI 1093 TCCGGA T^CCGGA
BsrDI 597 GCAATG GCAATG(2/0)
BstEII 2833 GGTGACC G^GT[ACGT]ACC
BstPI 2833 GGTGACC G^GT[ACGT]ACC
DraI 3915 TTTAAA TTT^AAA
DraIII 2588 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3368 AGCGCT AGC^GCT
EcoO65I 2833 GGTGACC G^GT[ACGT]ACC
FbaI 1062 TGATCA T^GATCA
FseI 457 GGCCGGCC GGCCGG^CC
HincII 3613 GTCAAC GT[CT]^[GA]AC
HindII 3613 GTCAAC GT[CT]^[GA]AC
HindIII 2323 AAGCTT A^AGCTT
KasI 1613 GGCGCC G^GCGCC
KpnI 3953 GGTACC GGTAC^C
MroI 1093 TCCGGA T^CCGGA
NarI 1613 GGCGCC GG^CGCC
PflFI 2919 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2105 ACCGGT A^CCGGT
SapI 697 GCTCTTC GCTCTTC(1/4)
SfoI 1613 GGCGCC GGC^GCC
Tth111I 2919 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3120 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BbsI 348 GAAGAC GAAGAC(2/6)
908 GTCTTC
BpuAI 348 GAAGAC GAAGAC(2/6)
908 GTCTTC
BsmBI 1646 GAGACG CGTCTC(1/5)
2782 GAGACG
BsmI 3120 GAATGC GAATGC(1/-1)
3539 GCATTC
BsrBI 1762 GAGCGG CCGCTC(-3/-3)
2083 GAGCGG
BssSI 872 CACGAG CACGAG(-5/-1)
3284 CTCGTG
EarI 698 CTCTTC CTCTTC(1/4)
1751 CTCTTC
Ksp632I 698 CTCTTC CTCTTC(1/4)
1751 CTCTTC
KspI 655 CCGCGG CCGC^GG
3642 CCGCGG
SacI 1403 GAGCTC GAGCT^C
1961 GAGCTC
SacII 655 CCGCGG CCGC^GG
3642 CCGCGG
StuI 1938 AGGCCT AGG^CCT
2874 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcgI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmaI SmlI Sse8387I StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI