##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccI AccII AclI AcyI
AgeI AocI Aor51HI AscI AvrII
BbeI BbrPI BcgI BlnI BlpI
Bpu1102I BsaHI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I Eco81I FauI FseI HaeII
Hin1I HpaI KasI KspI MluI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 1775 GGTACC G^GTACC
AccIII 2504 TCCGGA T^CCGGA
AflII 3013 CTTAAG C^TTAAG
AhdI 960 GACCCATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4418 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1775 GGTACC G^GTACC
AspEI 960 GACCCATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1900 TGCGCA TGC^GCA
BfrI 3013 CTTAAG C^TTAAG
BsaBI 1916 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2504 TCCGGA [AT]^CCGG[AT]
BseAI 2504 TCCGGA T^CCGGA
BsmBI 3472 GAGACG CGTCTC(1/5)
BspEI 2504 TCCGGA T^CCGGA
BspHI 924 TCATGA T^CATGA
BssSI 3696 CACGAG CACGAG(-5/-1)
DrdI 4605 GACTCTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 960 GACCCATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2543 GAATTC G^AATTC
EcoRV 1578 GATATC GAT^ATC
EcoT22I 2607 ATGCAT ATGCA^T
FspI 1900 TGCGCA TGC^GCA
HgaI 1929 GCGTC GACGC(5/10)
KpnI 1775 GGTACC GGTAC^C
MamI 1916 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1834 CAATTG C^AATTG
MroI 2504 TCCGGA T^CCGGA
MunI 1834 CAATTG C^AATTG
NcoI 774 CCATGG C^CATGG
NsiI 2607 ATGCAT ATGCA^T
PaeR7I 828 CTCGAG C^TCGAG
RcaI 924 TCATGA T^CATGA
SapI 2865 GAAGAGC GCTCTTC(1/4)
SpeI 1140 ACTAGT A^CTAGT
SspI 4641 AATATT AAT^ATT
SwaI 4282 ATTTAAAT ATTT^AAAT
XhoI 828 CTCGAG C^TCGAG
XmnI 4418 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 3326 ACACGT A^C[GA][CT]GT
4459 ACACGT
ApaI 208 GGGCCC GGGCC^C
4380 GGGCCC
BalI 497 TGGCCA TGG^CCA
3723 TGGCCA
BglII 1886 AGATCT A^GATCT
2849 AGATCT
BsaAI 840 CACGTA [CT]AC^GT[GA]
4460 CACGTA
BsmI 869 GAATGC GAATGC(1/-1)
2132 GCATTC
BstAPI 645 GCATATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2894 GCAGGTGCTGC
BstEII 309 GGTCACC G^GT[ACGT]ACC
3107 GGTAACC
BstPI 309 GGTCACC G^GT[ACGT]ACC
3107 GGTAACC
CfoI 833 GCGC GCG^C
1901 GCGC
DraI 4283 TTTAAA TTT^AAA
4483 TTTAAA
DsaI 726 CCGTGG C^C[GA][CT]GG
774 CCATGG
EcoO65I 309 GGTCACC G^GT[ACGT]ACC
3107 GGTAACC
HhaI 833 GCGC GCG^C
1901 GCGC
HinP1I 833 GCGC G^CGC
1901 GCGC
HincII 695 GTCAAC GT[CT]^[GA]AC
4632 GTTGAC
HindII 695 GTCAAC GT[CT]^[GA]AC
4632 GTTGAC
MluNI 497 TGGCCA TGG^CCA
3723 TGGCCA
MscI 497 TGGCCA TGG^CCA
3723 TGGCCA
SacI 160 GAGCTC GAGCT^C
2668 GAGCTC
SexAI 528 ACCAGGT A^CC[AT]GGT
1771 ACCAGGT
SphI 1494 GCATGC GCATG^C
1978 GCATGC
StuI 1075 AGGCCT AGG^CCT
4356 AGGCCT
XcmI 23 CCACTAGGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
124 CCAGGACAGCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AseI
AsnI AspHI AspI AvaI AvaII
BamHI BanI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XbaI XhoII